04-377 | Anti-MEK2 NT Antibody, rabbit monoclonal

04-377
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RFC, ICC, IH(P), IP, WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-377
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-MEK2 NT Antibody, rabbit monoclonal
      Alternate Names
      • MAP2K2
      • MAPKK2
      • MKK2
      • PRKMK2
      References
      Product Information
      FormatPurified
      PresentationIn 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
      Applications
      ApplicationAnti-MEK2 NT Antibody, Rabbit detects level of MEK2 NT & has been published & validated for use in FC, IC, IH(P), IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesSuggested dilutions:
      Immunoblot Analysis: 1:10,000
      Immunoprecipitation: 1:100
      Flow Cytometry: 1:40
      Immunohistochemistry: A 1:250-1:500
      Immunfluorescence Staining: 1:250-1:500
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to the N-terminal region of human MEK2.
      HostRabbit
      SpecificityRecognizes the N-terminal domain of MEK2.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
      Gene Symbol
      • MAP2K2
      • MEK2
      • PRKMK2
      • MAPKK2
      • MKK2
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P36507 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
      SIZE: 400 amino acids; 44424 Da
      SUBUNIT: Interacts with MORG1 (By similarity).
      PTM: MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
      DISEASE: SwissProt: P36507 # Defects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
      SIMILARITY: SwissProt: P36507 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight45 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-MEK2 NT (Rabbit monoclonal IgG) - 2107234 2107234
      Anti-MEK2 NT (Rabbit monoclonal IgG) - 2287117 2287117
      Anti-MEK2 NT (Rabbit monoclonal IgG) - 2326946 2326946
      Anti-MEK2 NT, Rabbit Monoclonal - 0611045818 0611045818

      References

      Reference overviewPub Med ID
      MEK1,2 response element mediates angiotensin II-stimulated plasminogen activator inhibitor-1 promoter activation.
      Chen, Hong-Chi and Feener, Edward P
      Blood, 103: 2636-44 (2004)  2004

      Show Abstract
      14656894 14656894
      Structures of human MAP kinase kinase 1 (MEK1) and MEK2 describe novel noncompetitive kinase inhibition
      Ohren, Jeffrey F, et al
      Nat Struct Mol Biol, 11:1192-7 (2004)  2004

      15543157 15543157
      A conserved docking site in MEKs mediates high-affinity binding to MAP kinases and cooperates with a scaffold protein to enhance signal transmission
      Bardwell, A J, et al
      J Biol Chem, 276:10374-86 (2001)  2001

      11134045 11134045

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies