CBL263 | Anti-MUC-1 Antibody, 12-mer epitope, clone VU 3C6

CBL263
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL263
      ReplacesCBL264
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-MUC-1 Antibody, 12-mer epitope, clone VU 3C6
      Alternate Names
      • Breast carcinoma-associated antigen DF3
      • Carcinoma-associated mucin
      • Episialin
      • H23AG
      • PEMT
      • Peanut-reactive urinary mucin
      • PUM
      • Polymorphic epithelial mucin
      • PEM
      • Tumor-associated epithelial membrane antigen
      • EMA
      • Tumor-associated mucin
      • CD227
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationAnti-MUC-1 Antibody, 12-mer epitope, clone VU 3C6 detects level of MUC-1 & has been published & validated for use in WB, IH, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blotting.

      Suitable for use on formalin fixed paraffin wax embedded tissue sections. HIER (Microwave, citrate buffer pH6.0) may be advantageous for some applications.

      Suitable for use on frozen tissue sections.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman breast cancer cell line ZR-75-1.
      Epitope12-mer epitope
      CloneVU 3C6
      HostMouse
      SpecificityThe antibody reacts with the extracellular 12-mer GVTSAPDTRPAP epitope of the MUC-1 tandem repeat.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined.
      Gene Symbol
      • MUC1
      • MUC-1/X
      • PEMT
      • MUC-1/SEC
      • PUM
      • MUC1/ZD
      • MUC-1
      • Episialin
      • episialin
      • EMA
      • PEM
      • H23AG
      • MAM6
      • CD227
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15941 # Isoform 7 behaves as a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling. Can bind to GRB2 adapter protein.
      SIZE: 1255 amino acids; 122072 Da
      SUBUNIT: The cleaved form (only the isoform 1) of MUC1 forms a tight heterodimer with the released C-terminal peptide (which is first secreted to be extracellular).
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 5: Secreted. & Isoform 9: Secreted.
      TISSUE SPECIFICITY: Expressed on ductal epithelial cells and on activated T-cells. Aberrantly glycosylated forms expressed in human epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 expressed only in tumoral cells.
      PTM: Highly glycosylated (N- and O-linked carbohydrates and sialic acid). In the 20 amino acid tandem repeat positions 5 (Ser), 6 (Thr), 14 (Thr), 15 (Ser) and 19 (Thr) are O-glycosylated (GalNAc). The average density of O-glycosylated sites within repeat peptides varies with cell differentiation from about 50% in lactation-associated MUC1 to over 90% in a variety of breast cancer cells. & Isoform 1 and isoform 7 undergoes transphosphorylation on serine and tyrosine residues.
      SIMILARITY: SwissProt: P15941 ## Contains 1 SEA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI- MUC-1 GLYCOPROTEIN MONOCLONAL ANTIBODY - 2283374 2283374
      MOUSE ANTI- MUC-1 GLYCOPROTEIN MONOCLONAL ANTIBODY -2514988 2514988
      MOUSE ANTI- MUC-1 GLYCOPROTEIN MONOCLONAL ANTIBODY -2673092 2673092
      MOUSE ANTI- MUC-1 GLYCOPROTEIN MONOCLONAL ANTIBODY -2726737 2726737

      References

      Reference overviewPub Med ID
      Telomerase-immortalized human mammary stem/progenitor cells with ability to self-renew and differentiate.
      Zhao X, Malhotra GK, Lele SM, Lele MS, West WW, Eudy JD, Band H, Band V
      Proc Natl Acad Sci U S A  107  14146-51. Epub 2010 Jul 26.  2010

      Show Abstract
      20660721 20660721

      Data Sheet

      Title
      Anti-MUC-1, 12-mer epitope, clone VU 3C6 - Data Sheet

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies