05-1500 | Anti-Mad1 Antibody, clone 9B10

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IPMPurifiedMonoclonal Antibody
      Catalogue Number05-1500
      DescriptionAnti-Mad1 Antibody, clone 9B10
      Alternate Names
      • MAD1 (mitotic arrest deficient
      • yeast
      • homolog)-like 1
      • MAD1 mitotic arrest deficient-like 1 (yeast)
      • MAD1-like 1
      • MAD1-like 1 protein
      • Mitotic arrest deficient-like protein 1
      • Mitotic checkpoint MAD1 protein-homolog
      • Tax-binding protein 181
      • mitotic-arrest deficient 1, yeast
      • homolog-like 1
      • tumor protein p53 inducible protein 9
      Background InformationMitotic arrest deficient-like 1 (yeast) is also known as MAD1 and MAD1L1. It is part of the MAD1 family which is crucial to development. MAD1 acts as a checkpoint gene during mitotic spindle assembly. During anaphase and telophase MAD1 is located at the spindle mid-zone of the cell, where it prevents anaphase until the chromosome is aligned at the metaphase plate. During metaphase MAD1 is located at the centrosome. MAD1 participates in cell cycle control and tumor suppression. MAD1 functions as a homodimer and interacts with MAD2L1. It is thought that MAD1 recruits to MAD2, which then promotes binding of MAD2 to CDC20.
      Product Information
      • HeLa nuclear extract
      PresentationPurified mouse monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine, (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Mad1 Antibody, clone 9B10 is an antibody against Mad1 for use in WB, IC & IP.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunoprecipitation

      Biological Information
      ImmunogenFull length recombinant Mad1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes Mad1.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • HsMAD1
      • MAD1
      • PIG9
      • TP53I9
      • TXBP181
      • hMAD1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Ref.2

      STRUCTURE:Homodimer. Heterodimerizes with MAD2L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex. Perturbation of the original MAD1L1-MAD2L1 structure by the spindle checkpoint may decrease MAD2L1 affinity for MAD1L1. CDC20 can compete with MAD1L1 for MAD2L1 binding, until the attachment and/or tension dampen the checkpoint signal, preventing further release of MAD2L1 on to CDC20. Also able to interact with the BUB1/BUB3 complex and the viral Tax protein. Ref.1

      SUBCELLULAR LOCATION: Nucleus. Note= From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Ref.1

      TISSUE SPECIFICITY: Expressed weakly at G0/G1 and highly at late S and G2/M phase.

      Induction Increased by TP53. Ref.2

      PTM: Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.1 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13

      DISEASE: Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.

      SIMILARITY: Belongs to the MAD1 family.

      SEQUENCE CAUTION: The sequence AAC52059.1 differs from that shown. Reason: Frameshift at position 663.

      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      1-5 µg/mL of this anti-Mad1, clone 9B10 detected Mad1 in HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Mad1, clone 9B10 - 2531919 2531919
      Anti-Mad1, clone 9B10 - NG1578247 NG1578247
      Anti-Mad1, clone 9B10 Monoclonal Antibody 3030282
      Anti-Mad1, clone 9B10_2842489 2842489


      Reference overviewSpeciesPub Med ID
      Sulforaphane causes epigenetic repression of hTERT expression in human breast cancer cell lines.
      Meeran, SM; Patel, SN; Tollefsbol, TO
      PLoS One  5  e11457  2010

      Show Abstract Full Text Article
      Human20625516 20625516
      Tripin/hSgo2 recruits MCAK to the inner centromere to correct defective kinetochore attachments.
      Huang, Haomin, et al.
      J. Cell Biol., 177: 413-24 (2007)  2007

      17485487 17485487
      Mapping the assembly pathways that specify formation of the trilaminar kinetochore plates in human cells.
      Liu, Song-Tao, et al.
      J. Cell Biol., 175: 41-53 (2006)  2006

      Show Abstract
      17030981 17030981