04-1555 | Anti-Mdmx Antibody, clone 8C6

04-1555
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, ICC, IHC, FC, ChIPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1555
      DescriptionAnti-Mdmx Antibody, clone 8C6
      Alternate Names
      • Double minute 4 protein
      • MDM4-related protein 1
      • Mdm2-like p53-binding protein
      • Mdm4 p53 binding protein homolog (mouse)
      • Mdm4, transformed 3T3 cell double minute 4, p53 binding protein
      • Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)
      • Protein Mdmx
      • double minute 4, human homolog of
      • p53-binding protein
      • mouse double minute 4 homolog
      • mouse double minute 4, human homolog of
      • p53-binding protein
      • p53-binding protein 2
        p53-binding protein Mdm4
      Background InformationMdmx is a Mdm2-related protein that like Mdm2 has been shown to bind to and inhibit p53, although not as well as Mdm2. This interaction with p53 occurs via a binding domain located in the N-terminal region of the protein. Mdmx has been shown to counteract Mdm2-dependent p53 degradation and to stabilize p53 in its inactive form. Although Mdmx has significant structural similarity to Mdm2 E3 ubiquitin ligase, most studies have not been able to ascribe E3 ligase function to Mdmx.
      References
      Product Information
      FormatPurified
      Control
      • MCF-7 cell lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-Mdmx Antibody, clone 8C6 is a Mouse Monoclonal Antibody for detection of Mdmx also known as MDM4-related protein 1, Mdm2-like p53-binding protein & has been validated in WB, IP, ICC, IHC, FC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunohistochemistry
      • Flow Cytometry
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory for this application. (Gilkes, D., et al. (2008). Molecular and Cellular Biology. 28(6):1999-2010.)

      Immunohistochemistry Analysis: A representative lot was used by an independent laboratory for this application. (Gilkes, D., et al. (2008). Molecular and Cellular Biology. 28(6):1999-2010.)

      Immunoprecipitation Analysis: A representative lot was used by an independent laboratoryfor this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)

      Flow Cytometry Analysis: A previous lot was used by an independent laboratory for this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to human Mdmx.
      EpitopeUnknown
      Clone8C6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Mdmx.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe human MDM4 gene(encodes Mdmx protein), which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Two transcript variants, one protein-coding and the other likely not to be protein-coding, have been found for this gene. [provided by RefSeq]
      Gene Symbol
      • HDMX
      • MDMX
      • MRP1
      • MDM4
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions.

      SUBUNIT STRUCTURE: Binds to p53, p73 and MDM2.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: In all tissues tested, with high levels in thymus.

      DOMAIN: Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.

      SEQUENCE SIMILARITIES: Belongs to the MDM2/MDM4 family.

      Contains 1 RanBP2-type zinc finger.

      Contains 1 RING-type zinc finger.

      Contains 1 SWIB domain.

      MASS SPECTROMETRY: Molecular mass is 54863.3 Da from positions 1 - 490. Determined by MALDI.
      Molecular Weight~ 80 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in MCF-7 cell lysate.

      Western Blot Analysis: 0.5 µg/ml of this antibody detected Mdmx on 10 µg of MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Mdmx, clone 8C6 2470930
      Anti-Mdmx, clone 8C6 - 2370848 2370848
      Anti-Mdmx, clone 8C6 - 2193332 2193332
      Anti-Mdmx, clone 8C6 - 2262740 2262740
      Anti-Mdmx, clone 8C6 - 2297291 2297291
      Anti-Mdmx, clone 8C6 - 2341023 2341023
      Anti-Mdmx, clone 8C6 - 2343592 2343592
      Anti-Mdmx, clone 8C6 - 2517757 2517757
      Anti-Mdmx, clone 8C6 - NG1753060 NG1753060
      Anti-Mdmx, clone 8C6 - NG1881826 NG1881826

      References

      Reference overviewApplicationPub Med ID
      A positive feedback between p53 and miR-34 miRNAs mediates tumor suppression.
      Okada, N; Lin, CP; Ribeiro, MC; Biton, A; Lai, G; He, X; Bu, P; Vogel, H; Jablons, DM; Keller, AC; Wilkinson, JE; He, B; Speed, TP; He, L
      Genes & development  28  438-50  2014

      Show Abstract
      Western Blotting24532687 24532687
      Maternal embryonic leucine zipper kinase (MELK) reduces replication stress in glioblastoma cells.
      Kig, C; Beullens, M; Beke, L; Van Eynde, A; Linders, JT; Brehmer, D; Bollen, M
      The Journal of biological chemistry  288  24200-12  2013

      Show Abstract
      Immunofluorescence23836907 23836907
      Decreased expression of miR-125b and miR-100 in oral cancer cells contributes to malignancy.
      Brian J Henson,Samsiddhi Bhattacharjee,Dawn M O'Dee,Eleanor Feingold,Susanne M Gollin
      Genes, chromosomes & cancer  48  2009

      Show Abstract Full Text Article
      19396866 19396866

      Newsletters / Publications

      Title
      Research Focus - Volume 5 2012