09-872 | Anti-Methylcytosine dioxygenase TET1 Antibody

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, ChIP-seqRbAffinity PurifiedPolyclonal Antibody
      Catalogue Number09-872
      DescriptionAnti-Methylcytosine dioxygenase TET1 Antibody
      Alternate Names
      • tet oncogene 1
      • Ten-eleven translocation 1 gene protein
      • CXXC-type zinc finger protein 6
      • methylcytosine dioxygenase TET1
      • CXXC finger 6
      • ten-eleven translocation-1
      • Leukemia-associated protein with a CXXC domain
      Background InformationMethylcytosine dioxygenase TET1 (mTET1) is a member of the TET family and contains a characteristic CXXC-type zinc finger domain. mTET1 binds methylcytosine (5mC), a minor, mammalian DNA base necessary to development and retrotransposon silencing, and acts as a catalyst to induce the conversion of 5mC to 5-hydromethylcytosine (hmC or 5hmC), a mammalian DNA base thought to be involved in chromatin structure and in the development of fetal brain, heart and lung tissues. Recent studies indicate that mTET1 regulates the levels of 5hmC, thereby influencing patterns in DNA methylation.
      Product Information
      FormatAffinity Purified
      • Mouse embryonic stem cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Methylcytosine dioxygenase TET1 Antibody is a Rabbit Polyclonal Antibody for detection of Methylcytosine dioxygenase TET1 also known as tet oncogene 1 & has been validated in WB, ChIP-seq.
      Key Applications
      • Western Blotting
      • ChIP-seq
      Application NotesWestern Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected Methylcytosine dioxygenase TET1 in 10 µg of mouse embryonic stem cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to mouse Methylcytosine dioxygenase TET1 near the C-terminus.
      EpitopeNear the C-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes methylcytosine dioxygenase TET1 near the C-terminus.
      Species Reactivity
      • Mouse
      Species Reactivity NoteDemonstrated to react with mouse.
      Other homologies: Rat (66% sequence homology), Human (61% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TET1
      • LCX1
      • CXXC6
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain (By similarity).

      CATALYTIC ACTIVITY: Methylcytosine + 2-oxoglutarate + O2 = 5-hydroxymethylcytosine + succinate + CO2.

      COFACTOR: Binds 1 Fe2+ ion per subunit (By similarity).

      SUBCELLULAR LOCATION: Nucleus (By similarity).

      SEQUENCE SIMILARITIES: Belongs to the TET family.

      Contains 1 CXXC-type zinc finger.

      SEQUENCE CAUTION: The sequence BAB27288.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight~220 kDa observed.
      Uncharacterized bands may be seen at ~32, 68, and 70 kDa.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse embryonic stem cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Methylcytosine dioxygenase TET1 in 10 µg of mouse embryonic stem cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Methylcytosine dioxygenase TET1 - 2142675 2142675
      Anti-Methylcytosine dioxygenase TET1 - 2388352 2388352
      Anti-Methylcytosine dioxygenase TET1 - 2465310 2465310
      Anti-Methylcytosine dioxygenase TET1 - 1963089 1963089
      Anti-Methylcytosine dioxygenase TET1 - 1988585 1988585
      Anti-Methylcytosine dioxygenase TET1 - 2009451 2009451
      Anti-Methylcytosine dioxygenase TET1 - 2053960 2053960
      Anti-Methylcytosine dioxygenase TET1 - 2089488 2089488
      Anti-Methylcytosine dioxygenase TET1 - 2227203 2227203
      Anti-Methylcytosine dioxygenase TET1 - 2298398 2298398


      Reference overviewApplicationPub Med ID
      Antidepressant-like effect of sodium butyrate is associated with an increase in TET1 and in 5-hydroxymethylation levels in the Bdnf gene.
      Wei, Y; Melas, PA; Wegener, G; Mathé, AA; Lavebratt, C
      The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP)  18  2015

      Show Abstract
      25618518 25618518
      Suppression of TET1-dependent DNA demethylation is essential for KRAS-mediated transformation.
      Wu, BK; Brenner, C
      Cell reports  9  1827-40  2014

      Show Abstract
      25466250 25466250
      PRDM14 promotes active DNA demethylation through the ten-eleven translocation (TET)-mediated base excision repair pathway in embryonic stem cells.
      Okashita, N; Kumaki, Y; Ebi, K; Nishi, M; Okamoto, Y; Nakayama, M; Hashimoto, S; Nakamura, T; Sugasawa, K; Kojima, N; Takada, T; Okano, M; Seki, Y
      Development (Cambridge, England)  141  269-80  2014

      Show Abstract
      24335252 24335252
      A dynamic CTCF chromatin binding landscape promotes DNA hydroxymethylation and transcriptional induction of adipocyte differentiation.
      Dubois-Chevalier, J; Oger, F; Dehondt, H; Firmin, FF; Gheeraert, C; Staels, B; Lefebvre, P; Eeckhoute, J
      Nucleic acids research  42  10943-59  2014

      Show Abstract
      25183525 25183525
      Enhancer transcribed RNAs arise from hypomethylated, Tet-occupied genomic regions.
      Pulakanti, K; Pinello, L; Stelloh, C; Blinka, S; Allred, J; Milanovich, S; Kiblawi, S; Peterson, J; Wang, A; Yuan, GC; Rao, S
      Epigenetics  8  1303-20  2013

      Show Abstract
      24135681 24135681
      Ten-eleven translocation 1 (Tet1) is regulated by O-linked N-acetylglucosamine transferase (Ogt) for target gene repression in mouse embryonic stem cells.
      Shi, FT; Kim, H; Lu, W; He, Q; Liu, D; Goodell, MA; Wan, M; Songyang, Z
      The Journal of biological chemistry  288  20776-84  2013

      Show Abstract
      23729667 23729667
      Tet Proteins Connect the O-Linked N-acetylglucosamine Transferase Ogt to Chromatin in Embryonic Stem Cells.
      Vella, Pietro, et al.
      Mol. Cell, (2013)  2013

      Show Abstract
      Immunoprecipitation23352454 23352454
      Cell cycle-dependent turnover of 5-hydroxymethyl cytosine in mouse embryonic stem cells.
      Otani, J; Kimura, H; Sharif, J; Endo, TA; Mishima, Y; Kawakami, T; Koseki, H; Shirakawa, M; Suetake, I; Tajima, S
      PloS one  8  e82961  2013

      Show Abstract
      24340069 24340069
      Fibroblast growth factor receptor 2 homodimerization rapidly reduces transcription of the pluripotency gene Nanog without dissociation of activating transcription factors.
      Santostefano, KE; Hamazaki, T; Pardo, CE; Kladde, MP; Terada, N
      The Journal of biological chemistry  287  30507-17  2012

      Show Abstract
      Western Blotting22787153 22787153
      Dynamic hydroxymethylation of deoxyribonucleic acid marks differentiation-associated enhancers.
      Sérandour, Aurélien A, et al.
      Nucleic acids research, (2012)  2012

      Show Abstract
      22730288 22730288


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