04-1041 | Anti-Musashi-1 (NT) Antibody, clone EP1302, rabbit monoclonal

04-1041
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IH(P), FC, ICC, IPRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1041
      DescriptionAnti-Musashi-1 (NT) Antibody, clone EP1302, rabbit monoclonal
      Alternate Names
      • Musashi (Drosophila) homolog 1
      • musashi 1
      • musashi homolog 1 (Drosophila)
      Background InformationIn mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. Musashi-1 (also known as Msi1), is selectivley expressed in neural progenitor cells, including neural stem cells. Outside the nervous system, Musashi-1 is a selective marker for intestinal stem or early lineage cells. Musashi-1 interacts with the Notch pathway during asymmetric cell division by binding the 3' UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi-1 is present.
      References
      Product Information
      FormatUnpurified
      Control
      • SHSY5Y cell lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Musashi-1 (N-term) Antibody, clone EP1302, Rabbit is an antibody against Musashi-1 (N-term) for use in WB, IH(P), FC, IC & IP.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Flow Cytometry
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected Musashi-1 in human brain tissue.
      Immunocytochemistry Analysis: A 1:250 - 500 dilution from a previous lot detected Musashi-1 in PC12 cells.
      Flow Cytometry: A 1:40 dilution of a previous lot was used in flow cytometry.
      Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues on the N-terminus of human Musashi-1.
      EpitopeN-terminus
      CloneEP1302
      HostRabbit
      SpecificityThis antibody recognizes Musashi-1 at and around the N-terminus.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
      Gene Symbol
      • Musashi-1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system By similarity.
      SUBCELLULAR LOCATION: Cytoplasm By similarity. Nucleus By similarity.
      TISSUE SPECIFICITY: Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
      DOMAIN: The first RNA recognition motif binds more strongly to RNA compared to the second one By similarity.
      SEQUENCE SIMILARITIES: Belongs to the Musashi family.
      Contains 2 RRM (RNA recognition motif) domains.
      Molecular Weight~39 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on SHSY5Y cell lysates.
      Western Blot Analysis: 1:2,000 dilution of this antibody was used to detect Musashi-1 in SHSY5Y cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Musashi-1 (N-term), clone EP1302, Rabbit Monoclonal - 2115999 2115999
      Anti-Musashi-1 (N-term), clone EP1302, Rabbit Monoclonal - 2377635 2377635
      Anti-Musashi-1 (N-term), clone EP1302, Rabbit Monoclonal - 2384774 2384774
      Anti-Musashi-1 (N-term), clone EP1302 - 2506290 2506290
      Anti-Musashi-1 (N-term), clone EP1302, Rabbit Monoclonal - 2020311 2020311
      Anti-Musashi-1 (N-term), clone EP1302, Rabbit Monoclonal - 2045859 2045859
      Anti-Musashi-1 (N-term), clone EP1302, Rabbit Monoclonal - 2192176 2192176
      Anti-Musashi-1 (N-term), clone EP1302, Rabbit Monoclonal - 2193353 2193353
      Anti-Musashi-1 (N-term), clone EP1302, Rabbit Monoclonal - 2272149 2272149
      Anti-Musashi-1 (N-term), clone EP1302, Rabbit Monoclonal - 2344721 2344721

      References

      Reference overviewPub Med ID
      Tumor suppressive microRNA-137 negatively regulates Musashi-1 and colorectal cancer progression.
      Smith, AR; Marquez, RT; Tsao, WC; Pathak, S; Roy, A; Ping, J; Wilkerson, B; Lan, L; Meng, W; Neufeld, KL; Sun, XF; Xu, L
      Oncotarget  6  12558-73  2015

      Show Abstract
      25940441 25940441

      Brochure

      Title
      Human Stem Cell Systems

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies