05-724 | Anti-Myc Tag Antibody, clone 4A6

05-724
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IF, IP, WB, ChIP-seqMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-724
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Myc Tag Antibody, clone 4A6
      Background InformationEpitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
      The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
      References
      Product Information
      FormatPurified
      Control
      • Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
      PresentationProtein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05 Sodium Azide, pH 7.4.
      Applications
      ApplicationThis Anti-Myc Tag Antibody, clone 4A6 is validated for use in ChIP, IC, IF, IP, WB for the detection of Myc Tag. This antibody has been cited in more than 25 published papers.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      • ChIP-seq
      Biological Information
      Immunogenpeptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc
      Clone4A6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts; also recognizes human Myc.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
      Gene Symbol
      • MYC
      • C-MYC
      • c-Myc
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
      SIZE: 439 amino acids; 48804 Da
      SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Phosphorylated by PRKDC.
      DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
      SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      Molecular WeightPredicted MW for human Myc: 49 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on Myc-tagged recombinant protein in sequence contexts not well recognized by anti-Myc Tag, clone 9E10 (Catalogue No. CBL430)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2 to 8°C for 1 year from date of shipment.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Myc Tag, clone 4A6 2476969
      Anti-Myc Tag, clone 4A6 2465252
      Anti-Myc Tag, clone 4A6 (mouse monoclonal IgG1) - DAM1414984 DAM1414984
      Anti-Myc Tag, clone 4A6 (mouse monoclonal IgG1) Monoclonal Antibody Q2909940
      Anti-Myc Tag, clone 4A6 - 2138018 2138018
      Anti-Myc Tag, clone 4A6 - 2387824 2387824
      Anti-Myc Tag, clone 4A6 - 2424717 2424717
      Anti-Myc Tag, clone 4A6 - 2436379 2436379
      Anti-Myc Tag, clone 4A6 - 0610042765 0610042765
      Anti-Myc Tag, clone 4A6 - 0612047379 0612047379

      References

      Reference overviewApplicationSpeciesPub Med ID
      Ubiquitously expressed transcript is a novel interacting protein of protein inhibitor of activated signal transducer and activator of transcription 2.
      Kong, X; Ma, S; Guo, J; Ma, Y; Hu, Y; Wang, J; Zheng, Y
      Molecular medicine reports  11  2443-8  2015

      Show Abstract
      25434787 25434787
      Dopaminergic and glutamatergic microdomains in a subset of rodent mesoaccumbens axons.
      Zhang, S; Qi, J; Li, X; Wang, HL; Britt, JP; Hoffman, AF; Bonci, A; Lupica, CR; Morales, M
      Nature neuroscience  18  386-92  2015

      Show Abstract
      25664911 25664911
      CHK1-driven histone H3.3 serine 31 phosphorylation is important for chromatin maintenance and cell survival in human ALT cancer cells.
      Chang, FT; Chan, FL; R McGhie, JD; Udugama, M; Mayne, L; Collas, P; Mann, JR; Wong, LH
      Nucleic acids research  43  2603-14  2015

      Show Abstract
      25690891 25690891
      Increased classical endoplasmic reticulum stress is sufficient to reduce chondrocyte proliferation rate in the growth plate and decrease bone growth.
      Kung, LH; Rajpar, MH; Preziosi, R; Briggs, MD; Boot-Handford, RP
      PloS one  10  e0117016  2015

      Show Abstract
      25693198 25693198
      Evidence for ubiquitin-regulated nuclear and subnuclear trafficking among Paramyxovirinae matrix proteins.
      Pentecost, M; Vashisht, AA; Lester, T; Voros, T; Beaty, SM; Park, A; Wang, YE; Yun, TE; Freiberg, AN; Wohlschlegel, JA; Lee, B
      PLoS pathogens  11  e1004739  2015

      Show Abstract
      25782006 25782006
      DRAM-3 modulates autophagy and promotes cell survival in the absence of glucose.
      Mrschtik, M; O'Prey, J; Lao, LY; Long, JS; Beaumatin, F; Strachan, D; O'Prey, M; Skommer, J; Ryan, KM
      Cell death and differentiation  22  1714-26  2015

      Show Abstract
      25929859 25929859
      Dispersed and conserved hydrophobic residues of HIV-1 Vif are essential for CBFβ recruitment and A3G suppression.
      Zhou, X; Han, X; Zhao, K; Du, J; Evans, SL; Wang, H; Li, P; Zheng, W; Rui, Y; Kang, J; Yu, XF
      Journal of virology  88  2555-63  2014

      Show Abstract
      24352440 24352440
      AKT regulates NPM dependent ARF localization and p53mut stability in tumors.
      Hamilton, G; Abraham, AG; Morton, J; Sampson, O; Pefani, DE; Khoronenkova, S; Grawenda, A; Papaspyropoulos, A; Jamieson, N; McKay, C; Sansom, O; Dianov, GL; O'Neill, E
      Oncotarget  5  6142-67  2014

      Show Abstract
      25071014 25071014
      Evolutionarily conserved requirement for core binding factor beta in the assembly of the human immunodeficiency virus/simian immunodeficiency virus Vif-cullin 5-RING E3 ubiquitin ligase.
      Han, X; Liang, W; Hua, D; Zhou, X; Du, J; Evans, SL; Gao, Q; Wang, H; Viqueira, R; Wei, W; Zhang, W; Yu, XF
      Journal of virology  88  3320-8  2014

      Show Abstract
      24390335 24390335
      Human rhinovirus 16 causes Golgi apparatus fragmentation without blocking protein secretion.
      Mousnier, A; Swieboda, D; Pinto, A; Guedán, A; Rogers, AV; Walton, R; Johnston, SL; Solari, R
      Journal of virology  88  11671-85  2014

      Show Abstract
      25100828 25100828

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      Life Science Research > Antibodies and Assays > Primary Antibodies