05-915 | Anti-N-Cadherin Antibody, clone 13A9

05-915
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IHC, IP, WBMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number05-915
      Replaces04-1126
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-N-Cadherin Antibody, clone 13A9
      Alternate Names
      • Cadherin-2
      • CD325
      • CDw325
      • N-cadherin
      • Neural cadherin
      Background InformationCadherin-2 (UniProt P19022; also known as CD325, CDw325, N-cadherin, Neural cadherin) is encoded by the CDH2 (also known as CDHN, NCAD) gene (Gene ID 1000) in human. Cadherins constitute a family of calcium-dependent cell-cell adhesion proteins that play important roles in the embryonic development and maintenance of normal tissue architecture. Cadherins are composed of an extracellular domain (a.a. 160-724 of human N-cadherin) with five homologous repeats that mediates adhesion, a single pass transmembrane domain (a.a. 725-745 of human N-cadherin), and a conserved cytoplasmic domain (a.a. 746-906 of human N-cadherin) that interacts with catenins to link cadherins to the actin cytoskeleton. In addition, a known Src substrate p120ctn also modulate the strength of cadherin-dependent adhesion by interacting with cadherins at their intracellular juxtamembrane domain. Cadherins are synthesized as precursor proteins that must be proteolytically cleaved to generate functional, mature proteins. Newly synthesized proN-cadherin (a.a. 1-906) is phosphorylated and proteolytically processed prior to transport to the plasma membrane. In addition, Plakoglobin (gamma-catenin) and beta-catenin associate only with phosphorylated proN-cadherin, whereas p120ctn can associate with both phosphorylated and non-phosphorylated proN-cadherin. The N-terminal signal and propeptide (a.a. 1-25 and 26-159 of of human N-cadherin) region is proteolytically removed and a core N-cadherin-catenin complex is assembled in the endoplasmic reticulum or Golgi compartment prior to localization at the plasma membrane where linkage to the actin cytoskeleton can be established.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • HeLa cell lysate
      PresentationMouse monoclonal immunoglobulin hybridoma culture supernatant containing 0.05% sodium azide before the addition of glycerol to 30%.
      Applications
      ApplicationDetect N-cadherin using this Anti-N-Cadherin Antibody, clone 13A9 validated for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A representative lot immunostained the extracellular matrix of the stable plaques and in the fibrous cap region rich in vascular smooth muscle cells (VSMCs) using patients-derived paraffin-embedded internal carotid artery tissue sections (Musumeci, G., et al. (2014). Histol. Histopathol. 29(6):707-719).
      Immunohistochemistry Analysis: A representative lot detected strong N-cadherin immunoreactivity in paraffin-embedded rectal cancer (RC) tissues with positive regional lymph node metastasis (RLNM) status, while only weak N-cadherin immunoreactivity was detected in RC with negative RLNM, and no N-cadherin staining was seen in normal colorectal epithelium (Fan, X.J., et al. (2012). Br. J. Cancer. 106(11):1735-1741).
      Immunohistochemistry Analysis: A representative lot detected N-cadherin immunoreactivity in formalin-fixed, paraffin-embedded hepatocellular carcinoma (HCC) tissue sections. A significant inverse correlation was found between RUNX3 and N-cadherin expression levels (Tanaka, S., et al. (2012). Int. J. Cancer. 131(11):2537-2546).
      Western Blotting Analysis: A representative lot detected an upregulated N-cadherin expression in CCL185 carcinoma cells following transient Epstein-Barr virus (EBV) infection. The EMT-like phenotype remained even after viral loss by culture selection pressure withdrawal (Queen, K.J., et al. (2013). Int. J. Cancer. 132(9):2076-2086).
      Western Blotting Analysis: A representative lot detected N-cadherin in Hep3B, Huh7, HLF and SK-Hep1 human hepatocellular carcinoma (HCC) cell lysates (Tanaka, S., et al. (2012). Int. J. Cancer. 131(11):2537-2546).
      Western Blotting Analysis: A representative lot detected both the unprocessed (pro-) and processed (mature) forms of N-cadherin in HeLa cell lysate (Wahl, J.K. 3rd., et al. (2003). J. Biol. Chem. 278(19):17269-17276).
      Western Blotting Analysis: A representative lot detected N-cadherin in WI-38 human fibroblast lysate, but not in JAr human placental choriocarcinoma cell lysate (Knudsen, K.A., et al. (1995). J. Cell Biol. 130(1):67-77).
      Immunocytochemistry Analysis: A representative lot detected N-cadherin immunoreactivity localized primarily at the cell-cell borders by fluorescent immunocytochemistry staining of 1% paraformaldehyde-fixed, methanol-permeabilized HeLa cells (Wahl, J.K. 3rd., et al. (2003). J. Biol. Chem. 278(19):17269-17276).
      Immunocytochemistry Analysis: A representative lot detected N-cadherin immunoreactivity colocalized with those of alpha- and beta-catenin by dual fluorescent immunocytochemistry staining of fixed WI-38 human fibroblasts (Knudsen, K.A., et al. (1995). J. Cell Biol. 130(1):67-77).
      Immunoprecipitation Analysis: Representative lots co-immunoprecipitated alpha-catenin, beta-catenin, and plakoglobin with N-cadherin from WI-38 human fibroblast and HeLa cell lysates (Wahl, J.K. 3rd., et al. (2003). J. Biol. Chem. 278(19):17269-17276; Knudsen, K.A., et al. (1995). J. Cell Biol. 130(1):67-77).
      Biological Information
      ImmunogenBacterially expressed human N-cadherin cytoplasmic domain MBP fusion protein (Knudsen, K.A., et al. (1995). J. Cell Biol. 130(1):67-77).
      EpitopeCytoplasmic domain.
      Clone13A9
      HostMouse
      SpecificityClone 13A9 recognizes N-cadherin, but not P-, E-, or M-cadherin (Knudsen, K.A., et al. (1995). J. Cell Biol. 130(1):67-77).
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDH2
      • CDHN
      • NCAD
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~140 kDa observed. Target band size appears larger than the calculated molecular weights of 82.03 kDa (mature) and 99.81/97.04 kDa (isoform 1/2 pro-form) due to posttranslational glycosylation and phosphorylation.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:1000-5000 dilution of this hybridoma culture supernatant detected N-cadherin in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-N-Cadherin, clone 13A9 - 2561084 2561084
      Anti-N-Cadherin, clone 13A9 - 30413 30413
      Anti-N-Cadherin, clone 13A9 -2602753 2602753
      Anti-N-Cadherin, clone 13A9 -2673038 2673038
      Anti-N-Cadherin, clone 13A9 -2705049 2705049
      Anti-N-Cadherin, clone 13A9 -2721666 2721666
      Anti-N-Cadherin, clone 13A9 -2724293 2724293
      AntiAnti -N-Cadherin, clone 13A9 -2802576 2802576

      References

      Reference overviewApplicationPub Med ID
      Epstein-Barr virus-induced epigenetic alterations following transient infection.
      Queen, KJ; Shi, M; Zhang, F; Cvek, U; Scott, RS
      International journal of cancer. Journal international du cancer  132  2076-86  2013

      Show Abstract
      Western Blotting23047626 23047626
      Runt-related transcription factor 3 reverses epithelial-mesenchymal transition in hepatocellular carcinoma.
      Shigetomi Tanaka,Hidenori Shiraha,Yutaka Nakanishi,Shin-Ichi Nishina,Minoru Matsubara,Shigeru Horiguchi,Nobuyuki Takaoka,Masaya Iwamuro,Junro Kataoka,Kenji Kuwaki,Hiroaki Hagihara,Junichi Toshimori,Hideki Ohnishi,Akinobu Takaki,Shinichiro Nakamura,Kazuhiro Nouso,Takahito Yagi,Kazuhide Yamamoto
      International journal of cancer. Journal international du cancer  131  2012

      Show Abstract
      22488108 22488108
      Epithelial-mesenchymal transition biomarkers and support vector machine guided model in preoperatively predicting regional lymph node metastasis for rectal cancer.
      Fan, XJ; Wan, XB; Huang, Y; Cai, HM; Fu, XH; Yang, ZL; Chen, DK; Song, SX; Wu, PH; Liu, Q; Wang, L; Wang, JP
      British journal of cancer  106  1735-41  2012

      Show Abstract
      Immunohistochemistry22538975 22538975
      Two novel EGFP insertion alleles reveal unique aspects of Pax2 function in embryonic and adult kidneys.
      Soofi, A; Levitan, I; Dressler, GR
      Developmental biology  365  241-50  2012

      Show Abstract
      Immunofluorescence22410172 22410172
      The cadherin-catenin complex as a focal point of cell adhesion and signalling: new insights from three-dimensional structures.
      Gooding, Jane M, et al.
      Bioessays, 26: 497-511 (2004)  2004

      Show Abstract
      15112230 15112230
      Structure-based models of cadherin-mediated cell adhesion: the evolution continues.
      Koch, A W, et al.
      Cell. Mol. Life Sci., 61: 1884-95 (2004)  2004

      Show Abstract
      15289931 15289931

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