06-1013 | Anti-NET5 Antibody

06-1013
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1013
      DescriptionAnti-NET5 Antibody
      Alternate Names
      • transmembrane protein 201
      • Spindle-associated membrane protein 1
      Background InformationTransmembrane protein 201, also known as NET5, is a transmembrane protein of the nuclear envelope that is homologous to spindle-associated membrane protein 1 (SAMP1) in humans. NET5 is thought to be expressed on the inner nuclear membrane during interphase and move to the polar regions of the mitotic spindle during mitosis.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human heart tissue lysate
      PresentationPurified rabbit polyclonal containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-NET5 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect NET5 also known as transmembrane protein 201.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected NET5 on 10 µg of rat heart tissue lysate.

      Immunocytochemistry Analysis: A representative lot was used by an independent laboratory that detected NET5 in U2OS cells expressing various NET-GFP fusions. (Wilkie, G., et al. (2011) Mol. Cell Proteomics).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human NET5.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Human, Mouse, and Rat. Predicted to react with Orangutan based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TMEM201
      • NET5
      • SAMP1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Isoform SAMP1 may define a distinct membrane domain in the vicinity of the mitotic spindle. Ref.3

      SUBCELLULAR LOCATION: Isoform SAMP1: Nucleus inner membrane; Multi-pass membrane protein. Note: The C-terminal of isoform SAMP1 is located on the nucleoplasmic side. During interphase, isoform SAMP1 is distributed in the inner nuclear membrane and during mitosis, it is found in the ER but it also localizes to the polar regions of the mitotic spindle. Ref.3
      Molecular Weight~72 and/or 43 kDa observed depending on lysate used. Isoform1: 72 kDa and Isoform 2: 43 kDa. Uncharacterized band at ~28 kDa may be observed in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human heart tissue lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected NET5 on 10 µg of human heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NET5 - NRG1922441 NRG1922441
      Anti-NET5 -2604832 2604832
      Anti-NET5 -2695270 2695270
      Anti-NET5 Polyclonal Antibody 2911442

      References

      Reference overviewApplicationPub Med ID
      Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery.
      Zuleger, N; Boyle, S; Kelly, DA; de las Heras, JI; Lazou, V; Korfali, N; Batrakou, DG; Randles, KN; Morris, GE; Harrison, DJ; Bickmore, WA; Schirmer, EC
      Genome biology  14  R14  2013

      Show Abstract
      23414781 23414781
      Several novel nuclear envelope transmembrane proteins identified in skeletal muscle have cytoskeletal associations.
      Wilkie, GS; Korfali, N; Swanson, SK; Malik, P; Srsen, V; Batrakou, DG; de las Heras, J; Zuleger, N; Kerr, AR; Florens, L; Schirmer, EC
      Molecular & cellular proteomics : MCP  10  M110.003129  2011

      Show Abstract Full Text Article
      Western Blotting20876400 20876400

      Brochure

      Title
      New Products: Volume 3, 2012