06-1027 | Anti-NET50 Antibody

06-1027
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, MWB, ICC, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1027
      DescriptionAnti-NET50 Antibody
      Alternate Names
      • dehydrogenase/reductase (SDR family) member 7
      • short chain dehydrogenase/reductase family 34C, member 1
      • Retinal short-chain dehydrogenase/reductase 4, Down-regulated in nephrectomized rat kidney #3
      Background InformationNET50 is a novel member of the short-chain dehydrogenases/reductases (SDR) family, a group of proteins comprised of oxidoreductases involved in the metabolism of prostaglandins, retinoids, and steroids. NET50 was identified in liver proteomics and is considered optimal for nuclear rim staining as RFP or HA fusions. The function of NET50 is as of yet undefined.
      References
      Product Information
      FormatAffinity Purified
      Control
      • COS7 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-NET50 Antibody is a rabbit polyclonal antibody for detection of NET50 also known as dehydrogenase/reductase (SDR family) member 7, Retinal short-chain dehydrogenase/reductase 4 & has been validated in WB, ICC,IHC(P).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blot (SNAP ID) Analysis: 2 µg/mL of this antibody has been shown to detect NET50 on 10 µg of COS7 cell lysate.

      Immunocytochemistry Analysis: 1:500 dilution of this anitbody has been shown to detect NET50 in A431 and HeLa cells.

      Immunohistochemistry Analysis: 1:300 dilution of this anitbody has been shown to detect NET50 in human hyperplastic prostate and colon crypt tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to rat NET50.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes NET50.
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat.
      Other homologies: Bovine (94% sequence homology). Canine (88% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CGI-86
      • retDSR4
      • NET50
      • DHRS7A
      • retSDR4
      • RETSDR4
      • SDR34C1
      • DHRS7
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummarySEQUENCE SIMILARITIES: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
      Molecular Weight~38 kDa observed. Uncharacterized band at ~80 kDa observed in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in COS7 cell lysate.

      Western Blot Analysis: 2 µg/mL of this antibody detected NET50 on 10 µg of COS7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications