04-234 | Anti-NFĸB p50 Antibody, clone E381, rabbit monoclonal

04-234
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, ICC, IHC, IH(P), WBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-234
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-NFĸB p50 Antibody, clone E381, rabbit monoclonal
      Alternate Names
      • Nuclear Factor kappa B
      Background InformationThe transcription factor NFĸB (Nuclear Factor kappa B) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response. NFĸB family represents a group of 5 structurally related and evolutionarily conserved proteins in mammals that include Rel (cRel), RelA (p65), RelB, NFĸB1 (p50 and its precursor p105), and NFĸB2 (p52 and its precursor p100). NFĸB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes, though the p50/p65 heterodimer of NFĸB appears to be the most abundant in cells. A critical component in NFĸB regulation is the IĸB Kinase (IKK) complex. NFĸB transcription factors exist in their inactive form and are retained in the cytoplasm by the bound inhibitory IĸB proteins. Upon stimulation, IĸB is rapidly phosphorylated and degraded, resulting in the release of the NFĸB complex. The p105 subunit is cleaved into its active p50 form. This cleavage exposes the NLS sequence on the p50 subunit. p50/p65 then translocates to the nucleus where it activates gene transcription. The wide variety of genes regulated by NFĸB includes those encoding cytokines, chemokines, adhesion molecules, acute phase proteins and inducible effector enzymes.
      References
      Product Information
      FormatCulture Supernatant
      PresentationUnpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect NFĸB p50 using this Anti-NFĸB p50 Antibody, clone E381 validated for use in FC, IC, IH, IH(P) & WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunocytochemistry: A 1:250-500 dilution of a previous lot was used in IC.

      Immunohistochemistry: A 1:250-500 dilution of a previous lot was used in IH.

      Flow Cytometry: A 1:30 dilution of a previous lot was used in FC.
      Biological Information
      ImmunogenKLH conjugated to NFB p105/50 linear peptide.
      CloneE381
      HostRabbit
      SpecificityThis antibody recognizes NFκB p50 and its p105 uncleaved precursor.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteProven to react with human, mouse and rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. [provided by RefSeq]
      Gene Symbol
      • NFKB
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFunction: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105. Ref.32
      Subunit structure: Component of the NF-kappa-B p65-p50 complex. Component of the NF-kappa-B p65-p50 complex. Homodimer; component of the NF-kappa-B p50-p50 complex. Component of the NF-kappa-B p105-p50 complex. Component of the NF-kappa-B p50-c-Rel complex. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Also interacts with MAP3K8. NF-kappa-B p50 subunit interacts with NCOA3 coactivator, which may coactivate NF-kappa-B dependent expression via its histone acetyltransferase activity. Interacts with DSIPI; this interaction prevents nuclear translocation and DNA-binding. Interacts with SPAG9 and UNC5CL. NFKB1/p105 interacts with CFLAR; the interaction inhibits p105 processing into p50. NFKB1/p105 forms a ternary complex with MAP3K8 and TNIP2. Interacts with GSK3B; the interaction prevents processing of p105 to p50. NFKB1/p50 interacts with NFKBIE. NFKB1/p50 interacts with NFKBIZ. Nuclear factor NF-kappa-B p50 subunit interacts with NFKBID By similarity.
      Subcellular location: Nucleus. Cytoplasm. Note= Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
      Induction: By phorbol ester and TNF-alpha.
      Domain: The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation.
      Glycine-rich region (GRR) appears to be a critical element in the generation of p50. Post-translational modification: While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p50 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.
      Phosphorylation at 'Ser-903' and 'Ser-907' primes p105 for proteolytic processing in response to TNF-alpha stimulation. Phosphorylation at 'Ser-927' and 'Ser-932' are required for BTRC/BTRCP-mediated proteolysis.
      Polyubiquitination seems to allow p105 processing.
      S-nitrosylation of Cys-61 affects DNA binding.
      Sequence similarities: Contains 7 ANK repeats.
      Contains 1 death domain.
      Contains 1 RHD (Rel-like) domain.

      Molecular Weight105/50 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa whole cell lysate.
      Western Blot Analysis: A 1:250-1:1,000 dilution of this lot was used to detect NFĸB p50 in HeLa whole cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NF#954;B p105/p50, clone E381 - 2136578 2136578
      Anti-NFkB p105/p50 clone E381 - DAM1647895 DAM1647895
      Anti-NFkB p105/p50 clone E381 - DAM1718122 DAM1718122
      Anti-NFkB p105/p50, clone E381 - 1997369 1997369
      Anti-NFkB p105/p50, clone E381 - 2090894 2090894
      Anti-NFkB p105/p50, clone E381 - 2204862 2204862
      Anti-NFkB p105/p50, clone E381 - 2210328 2210328
      Anti-NFkB p105/p50, clone E381 - 2283525 2283525
      Anti-NFkB p105/p50, clone E381 - 2328301 2328301
      Anti-NFkB p105/p50, clone E381 - DAM1811102 DAM1811102

      References

      Reference overviewApplicationPub Med ID
      Hyperoxia-induced hypertrophy and ion channel remodeling in left ventricle.
      Panguluri, SK; Tur, J; Fukumoto, J; Deng, W; Sneed, KB; Kolliputi, N; Bennett, ES; Tipparaju, SM
      American journal of physiology. Heart and circulatory physiology  304  H1651-61  2013

      Show Abstract
      Western Blotting23585127 23585127
      Dehydroxymethylepoxyquinomicin inhibits expression and production of inflammatory mediators in interleukin-1beta-induced human chondrocytes.
      Venera Cardile,Giuseppina Frasca,Massimo Libra,Silvia Caggia,Kazuo Umezawa,Annamaria Panico,Grazia Malaponte
      Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology  25  2010

      Show Abstract
      20332635 20332635

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies