05-432A4 | Anti-NR1 Antibody, C Terminus, Alexa Fluor®488 Conjugate | 05-432A4

05-432A4
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RICC, IHCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue Number05-432A4
      DescriptionAnti-NR1 Antibody, C Terminus, Alexa Fluor®488 Conjugate | 05-432A4
      Alternate Names
      • Glutamate [NMDA] receptor subunit zeta-1
      • N-methyl-D-aspartate receptor subunit NR1
      • NMD-R1
      Background InformationNR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteriztic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.
      References
      Product Information
      FormatAlexaFluor®488
      Control
      • Mouse adult brain tissue
      PresentationPurified mouse monoclonal IgG conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Applications
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      Biological Information
      ImmunogenRecombinant protein corresponding to rat NR1.
      EpitopeThis antibody maps to a region (a.a. 834-864) common to all NR1 isoforms
      HostMouse
      SpecificityThis antibody maps to a region (834-864) common to all NR1 isoforms and so should cross react with all NR1 isoforms.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Mouse and Rat. Predicted to react with Human based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySubunit of NMDA-preferring ionotropic glutamate receptors; may play a role in long term potentiation [RGD].
      Gene Symbol
      • Grin1
      • Nmdar1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.

      SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B, GRIN3A or GRIN3B and PPP2CB. Interacts with DLG4 and MPDZ

      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein, Cell junction › synapse › postsynaptic cell membrane. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Note: Enriched in post-synaptic plasma membrane and post-synaptic densities.

      POST-TRANSLATIONAL MODIFICATION: NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.

      SIZE: 938 amino acids; 105,509 Da
      Molecular WeightUniprot describes isoforms at 99-108 kDa. The unconjugated parent antibody (Cat. No. 05-432) has an observed molecular weight at ~130 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in mouse adult brain tissue.

      Immunohistochemistry Analysis: 1:100 dilution of this antibody detected NR1 in mouse adult brain tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies