05-901R | Anti-NR2A Antibody, clone A12W, rabbit monoclonal

05-901R
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, MWBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-901R
      DescriptionAnti-NR2A Antibody, clone A12W, rabbit monoclonal
      Alternate Names
      • N-methyl D-aspartate receptor subtype 2A
      • N-methyl-D-aspartate receptor channel, subunit epsilon-1
      • N-methyl-D-aspartate receptor subunit 2A
      • NMDA receptor subtype 2A
      • glutamate receptor, ionotropic, N-methyl D-aspartate 2A
      Background InformationNR2 or glutamate receptor, ionotropic, N-methyl D-aspartate 2 (NMDA2; NMDAR2) along with the NR1 subunit forms a heterodimer that make up the NMDA receptor. By alternative splicing there are various isoforms of NR2 classified as NR2A through D. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. The NR1 subunit binds glycine while the NR2 subunit binds glutamine for activation. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.
      References
      Product Information
      FormatPurified
      Control
      • Rat brain microsomal tissue lysate
      PresentationPurified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide and 40% Glycerol.
      Applications
      ApplicationThis Anti-NR2A Antibody, clone A12W is validated for use in WB for the detection of NR2A.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to the C-terminus of mouse NR2A.
      EpitopeC-terminus
      Cloneclone A12W
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes NR2A at the C-terminus.
      IsotypeIgG
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with rat. Predicted to react with mouse based on immunogen design. Predicted to react with human based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GRIN2A
      • hNR2A
      • Glutamate
      • NMDAR2A
      • NR2A
      Purification MethodProtein A
      UniProt Number
      UniProt SummaryFUNCTION: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.

      SUBUNIT STRUCTURE: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of AIP1, INADL and DLG4 (By similarity). Interacts with HIP1 and NETO1. Interacts with LRFN2 (By similarity).

      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein

      SEQUENCE SIMILARITIES: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. [View classification]
      Molecular Weight ~ 170 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in rat brain microsomal tissue lysate.

      Western Blot Analysis: 1:3000 dilution of this antibody detected NR2A on 10 µg of rat brain microsomal tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NR2A, clone A12W - 2115667 2115667
      Anti-NR2A, clone A12W - 2424820 2424820
      Anti-NR2A, clone A12W - 2446953 2446953
      Anti-NR2A, clone A12W - 2002738 2002738
      Anti-NR2A, clone A12W - 2036702 2036702
      Anti-NR2A, clone A12W - 2272159 2272159
      Anti-NR2A, clone A12W - NG1900686 NG1900686
      Anti-NR2A, clone A12W - NG1951084 NG1951084
      Anti-NR2A, clone A12W - NRG1815904 NRG1815904
      Anti-NR2A, clone A12W -2585831 2585831

      References

      Reference overviewApplicationPub Med ID
      Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway.
      Murata, Y; Constantine-Paton, M
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  5040-52  2013

      Show Abstract
      Western Blotting23486974 23486974