06-600 | Anti-NR2B Antibody

06-600
250 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MICC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-600
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-NR2B Antibody
      Alternate Names
      • N-methyl D-aspartate receptor subtype 2B
      • N-methyl-D-aspartate receptor subunit 2B
      • N-methyl-D-aspartate receptor subunit 3
      • glutamate receptor subunit epsilon-2
      • glutamate receptor, ionotropic
      • N-methyl D-aspartate 2B
      Background InformationNMDA receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B or NR2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA). Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease, epilepsy and ischemic neuronal cell death.
      References
      Product Information
      FormatPurified
      Control
      • Mouse brain tissue
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, and 0.05% sodium azide. Frozen solution.
      Applications
      ApplicationDetect NR2B using this Anti-NR2B Antibody validated for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      Immunogena.a. 1437-1456 of mature mouse NR2B or 1463-1482 of NR2B precursor; signal is a.a. residues 1-26 [KFNGSSNG HVYEKLSSIESDV]. This sequence is identical to a.a. 1437-1456 of mature rat NR2B and a.a. 1465-1484 of human NR3, containing a N-terminal lysine.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityNR2B; the immungen sequence is identical in mouse, rat, human, bovine, dog and chimp NR2B. Human NR2B is sometimes referred to as hNR3 in the literature.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse (M). Predicted to react with Bovine (B), Canine (Ca), Human (H), Monkey (Mk), Rat (R), Chimpanzee (Chp) based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryN-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain.
      Gene Symbol
      • MGC142178
      • MGC142180
      • NMDAR2B
      • NR3
      • hNR3
      • GRIN2B
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13224 # NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
      SIZE: 1484 amino acids; 166367 Da
      SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of INADL and DLG4 (By similarity).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
      SIMILARITY: SwissProt: Q13224 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
      Molecular Weight180 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on rat brain microsomal preparation (Catalog # 12-144).

      Western Blot Analysis:
      0.5-2 μg/mL of this lot of antibody detected NR2B (~180 kDa) in rat brain microsomal preparation (Catalog # 12-144).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Aliquot to avoid freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NR2B 2475735
      Anti-NR2B - 2116015 2116015
      Anti-NR2B - 15656 15656
      Anti-NR2B - 1962660 1962660
      Anti-NR2B - 1984840 1984840
      Anti-NR2B - 2019590 2019590
      Anti-NR2B - 2043522 2043522
      Anti-NR2B - 20740 20740
      Anti-NR2B - 2153151 2153151
      Anti-NR2B - 2297226 2297226

      References

      Reference overviewApplicationPub Med ID
      A novel approach identifies the first transcriptome networks in bats: a new genetic model for vocal communication.
      Rodenas-Cuadrado, P; Chen, XS; Wiegrebe, L; Firzlaff, U; Vernes, SC
      BMC genomics  16  836  2015

      Show Abstract
      26490347 26490347
      Simvastatin treatment enhances NMDAR-mediated synaptic transmission by upregulating the surface distribution of the GluN2B subunit.
      Parent, MA; Hottman, DA; Cheng, S; Zhang, W; McMahon, LL; Yuan, LL; Li, L
      Cellular and molecular neurobiology  34  693-705  2014

      Show Abstract
      24687455 24687455
      The nucleosome assembly protein TSPYL2 regulates the expression of NMDA receptor subunits GluN2A and GluN2B.
      Tsang, KH; Lai, SK; Li, Q; Yung, WH; Liu, H; Mak, PH; Ng, CC; McAlonan, G; Chan, YS; Chan, SY
      Scientific reports  4  3654  2014

      Show Abstract
      24413569 24413569
      Effect of D-cycloserine in conjunction with fear extinction training on extracellular signal-regulated kinase activation in the medial prefrontal cortex and amygdala in rat.
      Gupta, SC; Hillman, BG; Prakash, A; Ugale, RR; Stairs, DJ; Dravid, SM
      The European journal of neuroscience  37  1811-22  2013

      Show Abstract
      Western Blotting23551217 23551217
      A polyamine-deficient diet prevents oxaliplatin-induced acute cold and mechanical hypersensitivity in rats.
      Ferrier, J; Bayet-Robert, M; Pereira, B; Daulhac, L; Eschalier, A; Pezet, D; Moulinoux, JP; Balayssac, D
      PloS one  8  e77828  2013

      Show Abstract
      24204988 24204988
      The GluN3A subunit exerts a neuroprotective effect in brain ischemia and the hypoxia process.
      Wang, H; Yan, H; Zhang, S; Wei, X; Zheng, J; Li, J
      ASN neuro  5  231-42  2013

      Show Abstract
      23883441 23883441
      Enhanced expression of NR2B subunits of NMDA receptors in the inherited glaucomatous DBA/2J mouse retina.
      Dong, LD; Chen, J; Li, F; Gao, F; Wu, J; Miao, Y; Wang, Z
      Neural plasticity  2013  670254  2013

      Show Abstract
      24175101 24175101
      Dab1 is required for synaptic plasticity and associative learning.
      Trotter, J; Lee, GH; Kazdoba, TM; Crowell, B; Domogauer, J; Mahoney, HM; Franco, SJ; Müller, U; Weeber, EJ; D'Arcangelo, G
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  15652-68  2013

      Show Abstract
      Immunofluorescence24068831 24068831
      REST-dependent epigenetic remodeling promotes the developmental switch in synaptic NMDA receptors.
      Rodenas-Ruano, A; Chávez, AE; Cossio, MJ; Castillo, PE; Zukin, RS
      Nature neuroscience  15  1382-90  2012

      Show Abstract
      22960932 22960932
      Perturbing PSD-95 interactions with NR2B-subtype receptors attenuates spinal nociceptive plasticity and neuropathic pain.
      D'Mello, R; Marchand, F; Pezet, S; McMahon, SB; Dickenson, AH
      Molecular therapy : the journal of the American Society of Gene Therapy  19  1780-92  2011

      Show Abstract
      21427709 21427709

      Brochure

      Title
      Pathways and Biomarkers of Glutamatergic Synapse Flyer

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      Life Science Research > Antibodies and Assays > Primary Antibodies