06-520 | Anti-Na+/K+ ATPase α-1 Antibody

06-520
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, R, HICC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-520
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Na+/K+ ATPase α-1 Antibody
      Background InformationNa+/K+ ATPase α-1 is a human gene and functions to maintain Na+ and K+ across the cell membrane which is important for osmoregulation. It is a component of (Na+/K+) ATPase, a transporter that catalyzes the hydrolysis of ATP and transports sodium and potassium ions across the plasma membrane. It also partners with Src and other signaling proteins. Binding of ouabain and other cardiotonic steroids to the (Na+/K+) ATPase activates Src, resulting in the assembly and activation of multiple signaling cascades. Binding of ouabain also induces a positive inotropic effect. Diseases associated with ATPase are Thyrotoxic periodic paralysis and hyperthyroidism. (References: Wikipedia and http://www.bioportfolio.com/gene/476-ATP1A1.html)
      References
      Product Information
      FormatPurified
      Control
      • Rat Brain Microsomal Prep. lysate.
      PresentationPurified antibody in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl and 0.05% NaN3.
      Applications
      ApplicationThis Anti-Na+/K+ ATPase α-1 Antibody is validated for use in IC, IP, WB for the detection of Na+/K+ ATPase α-1.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged ATPase.
      EpitopeCytoplasmic domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes the α-1 subunit isoform of Na+/K+ ATPase.
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Rat
      • Human
      Species Reactivity NoteRat and Human. Expected to cross-react with Rat-100%, Mouse-99%; human, pig, rabbit, monkey-96%.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified.
      Gene Symbol
      • ATP1A1
      • G00-119-711
      • Na
      • +K
      • MGC3285
      • MGC51750
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05023 # This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
      SIZE: 1023 amino acids; 112896 Da
      SUBUNIT: Composed of three subunits: alpha (catalytic), beta and gamma.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      PTM: Phosphorylation on Tyr-10 modulates pumping activity (By similarity).
      SIMILARITY: SwissProt: P05023 ## Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.
      Molecular Weight112 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Immunoprecipitation on rat brain microsomal prep. lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Na+/K+ ATPase #945;-1 - 2120127 2120127
      Anti-Na+/K+ ATPase #945;-1 - 2041020 2041020
      Anti-Na+/K+ ATPase -1 - 2207269 2207269
      Anti-Na+/K+ ATPase -1 - 2299557 2299557
      Anti-Na+/K+ ATPase -1 - JBC1908437 JBC1908437
      Anti-Na+/K+ ATPase -1 - JBC1945030 JBC1945030
      Anti-Na+/K+ ATPase -1 - NG1574207 NG1574207
      Anti-Na+/K+ ATPase a-1 (rabbit polyclonal) Polyclonal Antibody Q2909962
      Anti-Na+/K+ ATPase α-1 2953774
      Anti-Na+/K+ ATPase α-1 - 2459626 2459626

      References

      Reference overviewApplicationPub Med ID
      Functional Characterization of ABCB4 Mutations Found in Low Phospholipid-Associated Cholelithiasis (LPAC).
      Kim, TH; Park, HJ; Choi, JH
      The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology  17  525-30  2013

      Show Abstract
      Western Blotting24381502 24381502
      Significance of aquaporins and sodium potassium ATPase subunits for expansion of the early equine conceptus.
      Budik, S; Walter, I; Tschulenk, W; Helmreich, M; Deichsel, K; Pittner, F; Aurich, C
      Reproduction (Cambridge, England)  135  497-508  2008

      Show Abstract
      18367510 18367510
      R7-binding protein targets the G protein beta 5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain.
      Nini, L; Waheed, AA; Panicker, LM; Czapiga, M; Zhang, JH; Simonds, WF
      BMC biochemistry  8  18  2007

      Show Abstract Full Text Article
      Western Blotting17880698 17880698
      Intrarenal octreotide treatment prevents sodium retention in liver cirrhotic rats: evidence for direct effects within the thick ascending limb of Henle's loop.
      Jonassen, TE; Christensen, S; Marcussen, N; Petersen, JS
      American journal of physiology. Renal physiology  291  F537-45  2006

      Show Abstract
      16418303 16418303
      Na+-K+-ATPase properties in rat heart and skeletal muscle 3 mo after coronary artery ligation.
      Barr, DJ; Green, HJ; Lounsbury, DS; Rush, JW; Ouyang, J
      Journal of applied physiology (Bethesda, Md. : 1985)  99  656-64  2005

      Show Abstract
      15817721 15817721
      Magnesium depletion enhances cisplatin-induced nephrotoxicity.
      H Lajer, M Kristensen, H H Hansen, S Nielsen, J Frøkiaer, L F Ostergaard, S Christensen, G Daugaard, T E N Jonassen
      Cancer chemotherapy and pharmacology  56  535-42  2005

      Show Abstract
      15947931 15947931
      Chloride, not sodium, stimulates expression of the {gamma} subunit of Na/K-ATPase and activates JNK in response to hypertonicity in mouse IMCD3 cells
      Capasso, J. M., et al
      Proc Natl Acad Sci U S A, 100:6428-6433 (2003)  2003

      Immunoblotting (Western)12746499 12746499
      Effects of renal denervation on tubular sodium handling in rats with CBL-induced liver cirrhosis.
      Thomas E N Jonassen, Lone Brønd, Malene Torp, Martin Graebe, Søren Nielsen, Ole Skøtt, Niels Marcussen, Sten Christensen
      American journal of physiology. Renal physiology  284  F555-63  2003

      Show Abstract
      12441306 12441306
      The mechanism by which interleukin-1 beta reduces net fluid absorption from the rat colon.
      Sawsan Ibrahim Kreydiyyeh, Rana Al-Sadi, Sawsan Ibrahim Kreydiyyeh, Rana Al-Sadi
      European cytokine network  13  358-63  2002

      Show Abstract
      12231480 12231480
      Purification of active Na+-K+-ATPase using a new ouabain-affinity column
      Yingst, D. R., et al
      Am J Physiol, 275:C1167-77 (1998)  1998

      Immunoblotting (Western)9755071 9755071

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies