05-369 | Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6

05-369
50 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Rb, M, Mk, Xn, Po, Ca, ShICC, IHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-369
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Na+/K+ ATPase α-1 Antibody, clone C464.6
      Alternate Names
      • ATPase
      • Na+/K+ transporting, alpha 3 polypeptide
      • Na(+)/K(+) ATPase alpha(III) subunit
      • Na(+)/K(+) ATPase alpha-3 subunit
      • Na+/K+ -ATPase alpha 3 subunit
      • Na+/K+ ATPase 3
      • Sodium pump subunit alpha-3
      • dystonia 12
      • sodium pump 3
      • sodium-potassium-ATPase
      • alpha 3 polypeptide
      • sodium/potassium-transporting ATPase alpha-3 chain
      Background InformationNa+/K+-ATPase (also known as the Na+/K+ pump or sodium-potassium pump) is an enzyme located in the plasma membrane (specifically an electrogenic transmembrane ATPase). It is found in the plasma membrane of virtually every human cell and is common to all cellular life. It helps maintain cell potential and regulate cellular volume.
      In order to maintain the cell potential, cells must keep a low concentration of sodium ions and high levels of potassium ions within the cell (intracellular). Outside cells (extracellular), there are high concentrations of sodium and low concentrations of potassium, so diffusion occurs through ion channels in the plasma membrane. In order to keep the appropriate concentrations, the sodium-potassium pump pumps sodium out and potassium in through active transport.
      References
      Product Information
      FormatPurified
      Control
      • Alpha 1 mRNA is present in all cell types examined.
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, 0.1% sodium azide. Frozen at
      -20ºC.
      Produced from a culture supernatant of a hybridoma produced by fusing Ag8 mouse myeloma cells with immunized Balb/c splenocytes. Clone C464.6 also called 6H.
      Applications
      ApplicationDetect Na+/K+ ATPase α-1 (sodium/potassium ATPase subunit alpha 1) using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6 validated for use in IC, IH, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      2 µg of a previous lot immuno-precipitated the α-1 subunit from rat brain microsomal preparations.

      Immunohistochemistry:
      1-10 µg/mL of a previous lot of this antibody was used in immunohistochemistry on ice-cold methanol-fixed tissue.

      1. Do not boil microsomes. Warming to 37ºC for 15 minutes is recommended.
      2. Microsomes from some tissues may contain sodium pump subunits which are below the level of detection by this antibody.
      3. Microsomes derived from tissues which are more difficult to prepare, such as smooth and skeletal muscle, frequently show non-specific cross-reactivity.
      Biological Information
      ImmunogenPurified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.
      CloneC464.6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the α-1 subunit isoform of Na+/K+ ATPase.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      • Rabbit
      • Mouse
      • Monkey
      • Xenopus
      • Pig
      • Canine
      • Sheep
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit.
      Gene Symbol
      • ATP1A3
      • MGC13276
      • RDP
      • EC 3.6.3.9
      • DYT12
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P13637 # This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
      SIZE: 1013 amino acids; 111749 Da
      SUBUNIT: Composed of three subunits: alpha (catalytic), beta and gamma.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      DISEASE: SwissProt: P13637 # Defects in ATP1A3 are the cause of dystonia-12 (DYT12) [MIM:128235]; also known as rapid-onset dystonia parkinsonism (RDP). DYT12 is an autosomal dominant form of dystonia- parkinsonism characterized by an unusually rapid evolution of signs and symptoms. Affected persons develop dystonia and parkinsonism between 14 and 45 years of age. The sudden onset of symptoms over hours to a few weeks, often associated with physical or emotional stress, suggests a trigger initiating a nervous system insult resulting in permanent neurologic disability.
      SIMILARITY: SwissProt: P13637 ## Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.
      Molecular Weight112 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in western blot on rat brain microsomal preparations.

      Western Blot Analysis:
      0.05-0.1 µg/mL of this lot detected the α-1 subunit in 20 µg of rat brain microsomal preparation (Catalog # 12-144). To reduce background, wash the blot with 0.05% Tween-20 in PBS for 15 minutes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Na+/K+ ATPase #945;-1, clone C464.6 - 2137077 2137077
      Anti-Na+/K+ ATPase -1, clone C464.6 - 1974024 1974024
      Anti-Na+/K+ ATPase -1, clone C464.6 - 2106954 2106954
      Anti-Na+/K+ ATPase -1, clone C464.6 - 2201565 2201565
      Anti-Na+/K+ ATPase -1, clone C464.6 - 2302178 2302178
      Anti-Na+/K+ ATPase -1, clone C464.6 - DAM1411276 DAM1411276
      Anti-Na+/K+ ATPase -1, clone C464.6 - DAM1476979 DAM1476979
      Anti-Na+/K+ ATPase -1, clone C464.6 - DAM1754422 DAM1754422
      Anti-Na+/K+ ATPase -1, clone C464.6 - DAM1770266 DAM1770266
      Anti-Na+/K+ ATPase -1, clone C464.6 - DAM1794271 DAM1794271

      References

      Reference overviewApplicationPub Med ID
      Potassium channel Kv1.3 is highly expressed by microglia in human Alzheimer's disease.
      Rangaraju, S; Gearing, M; Jin, LW; Levey, A
      Journal of Alzheimer's disease : JAD  44  797-808  2015

      Show Abstract
      25362031 25362031
      CD36 and Na/K-ATPase-α1 Form a Proinflammatory Signaling Loop in Kidney.
      Kennedy, David J, et al.
      Hypertension, 61: 216-24 (2013)  2013

      Show Abstract
      23172921 23172921
      Basolateral Mg2+ extrusion via CNNM4 mediates transcellular Mg2+ transport across epithelia: a mouse model.
      Yamazaki, D; Funato, Y; Miura, J; Sato, S; Toyosawa, S; Furutani, K; Kurachi, Y; Omori, Y; Furukawa, T; Tsuda, T; Kuwabata, S; Mizukami, S; Kikuchi, K; Miki, H
      PLoS genetics  9  e1003983  2013

      Show Abstract
      24339795 24339795
      Capsaicin induces NKCC1 internalization and inhibits chloride secretion in colonic epithelial cells independently of TRPV1.
      Bouyer, PG; Tang, X; Weber, CR; Shen, L; Turner, JR; Matthews, JB
      American journal of physiology. Gastrointestinal and liver physiology  304  G142-56  2013

      Show Abstract
      23139219 23139219
      The GAP portion of Pseudomonas aeruginosa type III secreted toxin ExoS upregulates total and surface levels of wild type CFTR.
      Tukaye, DN; Kwon, SH; Guggino, WB
      Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology  31  153-65  2013

      Show Abstract
      23428533 23428533
      The liver connexin32 interactome is a novel plasma membrane-mitochondrial signaling nexus.
      Fowler, SL; Akins, M; Zhou, H; Figeys, D; Bennett, SA
      Journal of proteome research  12  2597-610  2013

      Show Abstract
      23590695 23590695
      Attenuation of lithium-induced natriuresis and kaliuresis in P2Y₂ receptor knockout mice.
      Zhang, Y; Li, L; Kohan, DE; Ecelbarger, CM; Kishore, BK
      American journal of physiology. Renal physiology  305  F407-16  2013

      Show Abstract
      23739592 23739592
      Sortilin-related receptor SORCS3 is a postsynaptic modulator of synaptic depression and fear extinction.
      Breiderhoff, T; Christiansen, GB; Pallesen, LT; Vaegter, C; Nykjaer, A; Holm, MM; Glerup, S; Willnow, TE
      PloS one  8  e75006  2013

      Show Abstract
      24069373 24069373
      Na,K-ATPase alpha isoforms at the blood-cerebrospinal fluid-trigeminal nerve and blood-retina interfaces in the rat.
      Arakaki, X; McCleary, P; Techy, M; Chiang, J; Kuo, L; Fonteh, AN; Armstrong, B; Levy, D; Harrington, MG
      Fluids and barriers of the CNS  10  14  2013

      Show Abstract
      23497725 23497725
      SORLA-dependent and -independent functions for PACS1 in control of amyloidogenic processes.
      Burgert, T; Schmidt, V; Caglayan, S; Lin, F; Füchtbauer, A; Füchtbauer, EM; Nykjaer, A; Carlo, AS; Willnow, TE
      Molecular and cellular biology  33  4308-20  2013

      Show Abstract
      24001769 24001769

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies