07-565 | Anti-Necdin Antibody

07-565
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-565
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Necdin Antibody
      Background InformationNecdin is known to be a growth suppressor protein that is a member of the melanoma-associated antigen (MAGE) family and is mainly expressed in postmitotic neurons. Any expression of this protein ectopically halts growth. Necdin has been shown to play a role in modulating gene transcription. Nectin suppresses cell proliferation by interacting with hnRNP U. Necdin seems to be an important protein in the pathogenesis of Prader-Willi syndrome. Recently, Necdin has been linked to fetal and postnatal physiological myogenesis and regeneration of muscle.
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationAnti-Necdin Antibody is an antibody against Necdin for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 1-143 of human necdin.
      HostRabbit
      Specificityhuman necdin
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Broad species cross-reactivity expected based on sequence similarity.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons.
      Gene Symbol
      • NDN
      • necdin
      • HsT16328
      • Necdin.
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q99608 # Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle- promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences (By similarity).
      SIZE: 321 amino acids; 36086 Da
      SUBUNIT: Binds to the transactivation domains of E2F1 and p53. Binds also SV40 large T antigen and adenovirus E1A. Interacts with nucleobindin 1 and 2 (By similarity).
      SUBCELLULAR LOCATION: Perikaryon. Nucleus. Note=Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix.
      TISSUE SPECIFICITY: Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post- mitotic neurons.
      DISEASE: SwissProt: Q99608 # Defects in NDN may be a cause of the Prader-Willi syndrome (PWS) which is a contiguous gene syndrome resulting from inactivity of the paternal copies of a number of genes on 15q11, through deletion or disruption of these genes or maternal uniparental disomy 15. The PWS syndrome is characterized by muscular hypotonia, mental retardation, short stature, obesity, hypogonadotropic hypogonadism, and small hands and feet.
      SIMILARITY: SwissProt: Q99608 ## Contains 1 MAGE domain.
      Molecular Weight~38 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Necdin - 2275601 2275601
      Anti-Necdin - 26873 26873
      Anti-Necdin - DAM1780493 DAM1780493
      Anti-Necdin Polyclonal Antibody 2992396

      References

      Reference overviewApplicationSpeciesPub Med ID
      Putative tumour suppressor gene necdin is hypermethylated and mutated in human cancer.
      De Faveri, LE; Hurst, CD; Platt, FM; Taylor, CF; Roulson, JA; Sanchez-Carbayo, M; Knowles, MA; Chapman, EJ
      British journal of cancer  108  1368-77  2013

      Show Abstract
      Immunohistochemistry23549060 23549060
      Stochastic loss of silencing of the imprinted Ndn/NDN allele, in a mouse model and humans with prader-willi syndrome, has functional consequences.
      Rieusset, A; Schaller, F; Unmehopa, U; Matarazzo, V; Watrin, F; Linke, M; Georges, B; Bischof, J; Dijkstra, F; Bloemsma, M; Corby, S; Michel, FJ; Wevrick, R; Zechner, U; Swaab, D; Dudley, K; Bezin, L; Muscatelli, F
      PLoS genetics  9  e1003752  2013

      Show Abstract
      Immunofluorescence24039599 24039599
      Necdin modulates proliferative cell survival of human cells in response to radiation-induced genotoxic stress.
      Lafontaine, J; Tchakarska, G; Rodier, F; Mes-Masson, AM
      BMC cancer  12  234  2012

      Show Abstract
      Western Blotting22691188 22691188
      Necdin protects embryonic motoneurons from programmed cell death.
      Aebischer, J; Sturny, R; Andrieu, D; Rieusset, A; Schaller, F; Geib, S; Raoul, C; Muscatelli, F
      PloS one  6  e23764  2011

      Show Abstract
      ImmunohistochemistryMouse21912643 21912643
      Disorganized epithelial polarity and excess trophectoderm cell fate in preimplantation embryos lacking E-cadherin.
      Stephenson RO, Yamanaka Y, Rossant J
      Development  137  3383-91. Epub 2010 Sep 8.  2010

      Show Abstract
      20826529 20826529
      A single postnatal injection of oxytocin rescues the lethal feeding behaviour in mouse newborns deficient for the imprinted Magel2 gene.
      Schaller, F; Watrin, F; Sturny, R; Massacrier, A; Szepetowski, P; Muscatelli, F
      Human molecular genetics  19  4895-905  2010

      Show Abstract
      20876615 20876615
      The MAGE proteins: emerging roles in cell cycle progression, apoptosis, and neurogenetic disease.
      Barker, Philip A and Salehi, Amir
      J. Neurosci. Res., 67: 705-12 (2002)  2002

      11891783 11891783
      Necdin acts as a transcriptional repressor that interacts with multiple guanosine clusters.
      Matsumoto, K, et al.
      Gene, 272: 173-9 (2001)  2001

      Show Abstract
      11470523 11470523

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies