CBL212 | Anti-Neurofilament 200 kDa Antibody, clone RT97

CBL212
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, Ch, H, Ma, Po, R, RpIHC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL212
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurofilament 200 kDa Antibody, clone RT97
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal is presented in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationAnti-Neurofilament 200 kDa Antibody, clone RT97 detects level of Neurofilament 200 kDa & has been published & validated for use in IH, IH(P).
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesStaining of both frozen and paraffin wax embedded tissue sections

      Strong staining of neuronal processes in a variety of tissues

      Optimal working dilutions must be determined by the end user.
      Biological Information
      CloneRT97
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe clone RT97 recognizes the 200 kDa neurofilament polypeptide in a number of mammalian and avian species. Tumours specifically recognized include phaeochromocytoma, paraganglioma, ganglioneuroblastoma and other tumours of neuronal origin.

      KNOWN SPECIES CROSS REACTIVITY: Recognizes rat, chicken, pig and reptile
      IsotypeIgG1
      Species Reactivity
      • Avian
      • Chicken
      • Human
      • Mammals
      • Pig
      • Rat
      • Reptile
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFH
      • NF-H
      • NFH
      • KIAA0845
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
      SIZE: 1026 amino acids; 112480 Da
      PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
      SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN NEUROFILAMENT 200 kDa MONOCLONAL ANTIBODY 2977295
      MOUSE ANTI-HUMAN NEUROFILAMENT 200 kDa MONOCLONAL ANTIBODY - 2140007 2140007
      MOUSE ANTI-HUMAN NEUROFILAMENT 200 kDa MONOCLONAL ANTIBODY - 2143460 2143460
      MOUSE ANTI-HUMAN NEUROFILAMENT 200 kDa MONOCLONAL ANTIBODY - 2450207 2450207
      MOUSE ANTI-HUMAN NEUROFILAMENT 200 kDa MONOCLONAL ANTIBODY - 2032230 2032230
      MOUSE ANTI-HUMAN NEUROFILAMENT 200 kDa MONOCLONAL ANTIBODY - 2320029 2320029
      MOUSE ANTI-HUMAN NEUROFILAMENT 200 kDa MONOCLONAL ANTIBODY - 2844441 2844441
      MOUSE ANTI-HUMAN NEUROFILAMENT 200 kDa MONOCLONAL ANTIBODY - 2888833 2888833
      MOUSE ANTI-HUMAN NEUROFILAMENT 200 kDa MONOCLONAL ANTIBODY -2566941 2566941
      MOUSE ANTI-HUMAN NEUROFILAMENT 200 kDa MONOCLONAL ANTIBODY -2700207 2700207

      References

      Reference overviewApplicationPub Med ID
      Strong cortical and spinal cord transduction after AAV7 and AAV9 delivery into the cerebrospinal fluid of nonhuman primates.
      Samaranch, L; Salegio, EA; San Sebastian, W; Kells, AP; Bringas, JR; Forsayeth, J; Bankiewicz, KS
      Human gene therapy  24  526-32  2013

      Show Abstract
      Immunofluorescence23517473 23517473
      Genotype-selective phenotypic switch in primary afferent neurons contributes to neuropathic pain.
      Nitzan-Luques A, Devor M, Tal M.
      Pain  152  2413-26  2011

      Show Abstract
      21872992 21872992
      2-(Cyclohexylamino)-1-(4-cyclopentylpiperazin-1-yl)-2-methylpropan-1-one, a novel compound with neuroprotective and neurotrophic effects in vitro.
      H Elbrønd-Bek,A Wellejus,N M Kelly,M S Weidner,S H Jørgensen
      Neurochemistry international  59  2011

      Show Abstract
      21854820 21854820
      Oxaliplatin-induced loss of phosphorylated heavy neurofilament subunit neuronal immunoreactivity in rat DRG tissue.
      Jamieson SM, Subramaniam J, Liu JJ, Jong NN, Ip V, Connor B, McKeage MJ
      Molecular pain  5  66  2009

      Show Abstract Full Text Article
      19922644 19922644
      Tissue engineering a complete vaginal replacement from a small biopsy of autologous tissue.
      Roger E De Filippo,Roger E De Philippo,Colin E Bishop,Luiz Freitas Filho,James J Yoo,Anthony Atala
      Transplantation  86  2008

      Show Abstract
      18645481 18645481
      Signals for the initiation and termination of synthesis of the viral strand of bacteriophage f1.
      Dotto, G P, et al.
      Cold Spring Harb. Symp. Quant. Biol., 47 Pt 2: 717-22 (1983)  1983

      6305583 6305583
      Monoclonal antibodies show that neurofibrillary tangles and neurofilaments share antigenic determinants.
      Anderton, B H, et al.
      Nature, 298: 84-6 (1982)  1982

      6178036 6178036

      Data Sheet

      Title
      Anti-Neurofilament 200 kD, clone RT97 - Data Sheet

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies