04-236 | Anti-Nibrin/NBS1 Antibody, clone Y112, rabbit monoclonal

04-236
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, IHC, IH(P), IP, WBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-236
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Nibrin/NBS1 Antibody, clone Y112, rabbit monoclonal
      Alternate Names
      • Cell cycle regulatory protein p95
      • Nijmegen breakage syndrome 1 (nibrin)
      • Nijmegen breakage syndrome protein 1
      • p95 protein of the MRE11/RAD50 complex
      Background InformationNibrin (NBS1, NBN) is known to be a key regulator of DNA Damage repair by forming a complex with Rad50 and MRE11. This complex, more commonly referred to as MRN (Mre11/Rad50/Nibrin) is important in double-stranded break repair, DNA recombination, cell cycle checkpoint control, and meiosis. This complex quickly recognizes double stranded break (DSB) sites and quickly relocates to these sites. Nibrin senses DNA damage by recruiting ATM, ATR, and DNA-PK to the damage sites and activation their functions.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • HeLa whole cell lysate.
      PresentationUnpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Nibrin/NBS1 Antibody, clone Y112 detects level of Nibrin/NBS1 & has been published & validated for use in IC, IH, IH(P), IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry: A 1:50 dilution of a previous lot was used in IH.

      Immunoprecipitation: A 1:60 dilution of a previous lot was used in IP.

      Immunocytochemistry: A 1:50 dilution of a previous lot was used in IC.
      Biological Information
      ImmunogenKLH conjugated to Nibrin/NBS1 linear peptide.
      EpitopeC-terminus
      CloneY112
      HostRabbit
      SpecificityThis antibody recognizes Nibrin/NBS1.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteProven to react with human and mouse. Not expected to react with rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq]
      Gene Symbol
      • AT-V1
      • AT-V2
      • ATV
      • FLJ10155
      • MGC87362
      • NBS
      • NBS1
      • P95
      • nibrin
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFunction: Component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Ref.9 Ref.14 Ref.19
      Subunit structure: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50 and MRE11A By similarity. Interacts with histone H2AFX this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with HJURP, KPNA2 and TERF2.
      Subcellular location: NucleusBy similarity. TelomereBy similarity. Note= Localizes to discrete nuclear foci after treatment with genotoxic agents By similarity.
      Tissue specificity: Ubiquitous. Expressed at high levels in testis.
      Domain: The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage. Ref.18
      The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex. Ref.18
      The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.
      Post-translational modification: Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.
      Involvement in disease: Defects in NBN are the cause of Nijmegen breakage syndrome (NBS) [MIM:251260]. NBS is an autosomal recessive syndrome characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, immunodeficiency and predisposition to cancer, particularly to lymphoid malignancies.Defects in NBN are a cause of genetic susceptibility to breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer. Defects in NBN may be associated with aplastic anemia [MIM:609135]. Aplastic anemia is a disease of bone-marrow failure characterized by peripheral pancytopenia and marrow hypoplasia. Most of the cases of aplastic anemia are idiopathic, some are familial and some are due to a viral infection or to exposure to chemicals and radiation. Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).
      Miscellaneous: In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells.
      Sequence similarities: Contains 1 BRCT domain.
      Contains 1 FHA domain.
      Sequence caution: The sequence AAI08651.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence starting in position 550.
      Molecular Weight95 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa whole cell lysate.

      Western Blot Analysis: A 1:250 dilution of this lot was used to detect Nibrin/NBS1 in HeLa whole cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Nibrin/NBS1, clone Y112 - 2123628 2123628
      Anti-Nibrin/NBS1, clone Y112 - 2135060 2135060
      Anti-Nibrin/NBS1, clone Y112 - 2395636 2395636
      Anti-Nibrin/NBS1, clone Y112 - 2468119 2468119
      Anti-Nibrin/NBS1, clone Y112 - 2068537 2068537
      Anti-Nibrin/NBS1, clone Y112 - 2197149 2197149
      Anti-Nibrin/NBS1, clone Y112 - 2276282 2276282
      Anti-Nibrin/NBS1, clone Y112 - NG1572365 NG1572365
      Anti-Nibrin/NBS1, clone Y112 - NG1915289 NG1915289
      Anti-Nibrin/NBS1, clone Y112 -2505158 2505158

      References

      Reference overviewPub Med ID
      Activation induced deaminase C-terminal domain links DNA breaks to end protection and repair during class switch recombination.
      Zahn, A; Eranki, AK; Patenaude, AM; Methot, SP; Fifield, H; Cortizas, EM; Foster, P; Imai, K; Durandy, A; Larijani, M; Verdun, RE; Di Noia, JM
      Proceedings of the National Academy of Sciences of the United States of America  111  E988-97  2014

      Show Abstract
      24591601 24591601

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies