05-616-C | Anti-Nibrin/Nbs1 Antibody, clone EE15 (Ascites Free)

05-616-C
200 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-616-C
      Replaces05-616
      DescriptionAnti-Nibrin/Nbs1 Antibody, clone EE15 (Ascites Free)
      Alternate Names
      • Nibrin
      • Cell cycle regulatory protein p95
      • Nijmegen breakage syndrome protein 1
      • p95 protein of the MRE11/RAD50 complex
      Background InformationNibrin (UniProt O60934; also known as Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, p95 protein of the MRE11/RAD50 complex) is encoded by the NBN (also known as AT-V1, AT-V2, ATV, NBS, NBS1, P95) gene (Gene ID 4683) in human. Nibrin is a component of the MRN (MRE11/RAD50/NBN) complex involved in early steps of DNA double strand breaks (DSBs). The phosphorylation of H2AX pSer139 (γ-H2AX) found at the DNA-damaged sites, for example, is mediated by a direct interaction between Nibrin and the phosphorylated mediator of the DNA damage checkpoint 1 (MDC1). ATM, CtIP/RBBP8, Tip60, BRCA1, and SMC1 are also known interaction partners of Nibrin. Nibrin contains a fork-head associated (FHA) domain and two BRCA1 C-terminal (BRCT) tandem domains in its N-terminal region, followed by two MRE11-binding motifs and an ATM-binding motif in its C-terminal region. The Ser278 and Ser343 residues are known to be targeted for phosphorylation by ATM in response to ionizing-radiation (IR). NBN gene mutations are responsible for Nijmegen breakage syndrome (NBS), a disorder characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, immunodeficiency and predisposition to cancer.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Nibrin/Nbs1 Antibody, clone EE15 (Ascites Free) is an antibody against Nibrin/Nbs1 for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected Nibrin/Nbs1 in A431 cells.
      Western Blotting Analysis: A representative lot detected a shift of Nbs apparent molecular weight in lysates from gamma-irradiated human cells (U2OS, IMR90, HEK293, BJAB) (Wu, X., et al. (2000). Nature. 405(6785):477-482).
      Immunocytochemistry Analysis: A representative lot detected NBS1-positive nuclear foci by fluorescent immunocytochemistry (Wu, X., et al. (2000). Nature. 289(5476):11).
      Immunoprecipitation Analysis: 4 μg of this antibody immunoprecipitated Nibrin/Nbs1 from 500 μg of Jurkat RIPA lysate.
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 420-774 of human Nibrin/Nbs1.
      EpitopeAmino acids 420-774.
      CloneEE15
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityRecognizes Nibrin/Nbs1, Mr 95kDa. A non-specific protein was also detected at Mr 33kDa in some lysates, but not Jurkat cell lysate.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Not Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NBN
      • NBS
      • NBS1
      • P95
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~95 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Nibrin/Nbs1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 μg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies