06-284 | Anti-Nitrotyrosine Antibody

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AICC, IHC, IP, WBRbPurifiedPolyclonal Antibody
      Catalogue Number06-284
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Nitrotyrosine Antibody
      Background InformationNitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others.
      Product Information
      • Immunohistochemistry: Human Alzheimer disease brain sections; mouse liver
        Immunoblotting: Nitrated-BSA
        Indirect ELISA: Nitrated-BSA
        Indirect immunofluorescence: A549 cells metabolically labeled with 3-nitrotyrosine
      PresentationProtein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      ApplicationThis Anti-Nitrotyrosine Antibody is published in more than 80 citations and validated for use in Immunocytochemistry (IC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB) for the detection of Nitrotyrosine.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenNitrated KLH
      SpecificityRecognizes proteins which are nitrated.
      Species Reactivity
      • All
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoAffinity Purified
      Molecular WeightPredicted MW for nitrated-BSA: 85 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on nitrotyrosine immunoblotting controls (Catalog #12-354)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Nitrotyrosine - 2144405 2144405
      Anti-Nitrotyrosine - 2459610 2459610
      Anti-Nitrotyrosine - 16486 16486
      Anti-Nitrotyrosine - 16941 16941
      Anti-Nitrotyrosine - 17108 17108
      Anti-Nitrotyrosine - 17471 17471
      Anti-Nitrotyrosine - 18272 18272
      Anti-Nitrotyrosine - 18635 18635
      Anti-Nitrotyrosine - 19132 19132
      Anti-Nitrotyrosine - 2019733 2019733


      Reference overviewApplicationSpeciesPub Med ID
      Schisandrae Fructus Supplementation Ameliorates Sciatic Neurectomy-Induced Muscle Atrophy in Mice.
      Kim, JW; Ku, SK; Kim, KY; Kim, SG; Han, MH; Kim, GY; Hwang, HJ; Kim, BW; Kim, CM; Choi, YH
      Oxidative medicine and cellular longevity  2015  872428  2015

      Show Abstract
      26064425 26064425
      Predictive Potential of Twenty-Two Biochemical Biomarkers for Coronary Artery Disease in Type 2 Diabetes Mellitus.
      Pereira, EC; Bertolami, MC; Faludi, AA; Monte, O; Xavier, HT; Pereira, TV; Abdalla, DS
      International journal of endocrinology  2015  146816  2015

      Show Abstract
      26089875 26089875
      Neurodegeneration and Vision Loss after Mild Blunt Trauma in the C57Bl/6 and DBA/2J Mouse.
      Bricker-Anthony, C; Rex, TS
      PloS one  10  e0131921  2015

      Show Abstract
      26148200 26148200
      IL-13 overexpression in mouse lungs triggers systemic genotoxicity in peripheral blood.
      Chapmana, AM; Malkin, DJ; Camacho, J; Schiestl, RH
      Mutation research  769  100-7  2014

      Show Abstract
      25400503 25400503
      Direct exposure of non-equilibrium atmospheric pressure plasma confers simultaneous oxidative and ultraviolet modifications in biomolecules.
      Okazaki, Y; Wang, Y; Tanaka, H; Mizuno, M; Nakamura, K; Kajiyama, H; Kano, H; Uchida, K; Kikkawa, F; Hori, M; Toyokuni, S
      Journal of clinical biochemistry and nutrition  55  207-15  2014

      Show Abstract
      25411528 25411528
      Liver X receptor agonist treatment attenuates cardiac dysfunction in type 2 diabetic db/db mice.
      He, Q; Pu, J; Yuan, A; Yao, T; Ying, X; Zhao, Y; Xu, L; Tong, H; He, B
      Cardiovascular diabetology  13  149  2014

      Show Abstract
      25416469 25416469
      2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is selectively toxic to primary dopaminergic neurons in vitro.
      Griggs, AM; Agim, ZS; Mishra, VR; Tambe, MA; Director-Myska, AE; Turteltaub, KW; McCabe, GP; Rochet, JC; Cannon, JR
      Toxicological sciences : an official journal of the Society of Toxicology  140  179-89  2014

      Show Abstract
      24718704 24718704
      Mycobacteria counteract a TLR-mediated nitrosative defense mechanism in a zebrafish infection model.
      Elks, PM; van der Vaart, M; van Hensbergen, V; Schutz, E; Redd, MJ; Murayama, E; Spaink, HP; Meijer, AH
      PloS one  9  e100928  2014

      Show Abstract
      24967596 24967596
      Molecular changes and vision loss in a mouse model of closed-globe blast trauma.
      Bricker-Anthony, C; Hines-Beard, J; Rex, TS
      Investigative ophthalmology & visual science  55  4853-62  2014

      Show Abstract
      24994864 24994864
      Pomegranate juice exacerbates oxidative stress and nigrostriatal degeneration in Parkinson's disease.
      Tapias, V; Cannon, JR; Greenamyre, JT
      Neurobiology of aging  35  1162-76  2014

      Show Abstract
      24315037 24315037


      Pathways and Biomarkers of Oxidative Stress

      Technical Info

      White Paper- Modern Methods in Oxidative Stress Research