06-284-AF647 | Anti-Nitrotyrosine Antibody AlexaFluor® 647 Conjugate

06-284-AF647
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCRbAlexaFluor®647Polyclonal Antibody
      Description
      Catalogue Number06-284-AF647
      DescriptionAnti-Nitrotyrosine Antibody AlexaFluor® 647 Conjugate
      Alternate Names
      • Nitrotyrosine AlexaFluor® 647 Conjugate
      Background InformationNitrotyrosine is produced by the action of reactive nitrogen species, such as peroxinitrite and nitrogen dioxide on tyrosine residues in proteins. Nitrotyrosine is an indicator of cell damage, inflammation, and nitric oxide production. Both free tyrosine and protein bound tyrosine residues are prone to nitration.
      In several pathological conditions, oxidative stress is increased resulting in the formation of super oxide and nitric oxide that react to form peroxynitrite (ONOO-), which is a highly reactive molecule. It causes oxidation of proteins and lipids and DNA damage, which results in loss of protein and lipid function and can increase the probability of gene mutations. Higher incidence of protein nitration has been reported in diabetes, atherosclerotic plaques, and rheumatoid arthritis, among others. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected cells and tissues by immunocytochemical techniques using antibodies developed against the nitrotyrosine.
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified rabbit polyclonal antibody in buffer containing PBS, pH7.1, and 15 mg/mL BSA with 0.1% sodium azide.
      Applications
      ApplicationAnti-Nitrotyrosine AlexaFluor® 647 Conjugate, Cat. No. 06-284-AF647, is a rabbit polyclonal antibody that detects tyrosine nitrated proteins and has been tested for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antibody (Cat. No. 06-284) is shown to be suitable also for Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation.
      Biological Information
      ImmunogenNitrated Keyhole Limpet hemocyanain (KLH)
      EpitopeNitrated Tyrosine
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects tyrosine nitrated proteins.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with all species.
      Antibody TypePolyclonal Antibody
      Purification MethodAffinity Purfied
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 treated with peroxytnitrite.

      Immunocytochemistry Analysis: 1:100 dilution of this antibody detected Nitrotyrosine in A431 cells treated with 5 mM peroxytnitrite.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications