06-284-AF647 | Anti-Nitrotyrosine Antibody AlexaFluor® 647 Conjugate

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCRbAlexaFluor®647Polyclonal Antibody
      Catalogue Number06-284-AF647
      DescriptionAnti-Nitrotyrosine Antibody AlexaFluor® 647 Conjugate
      Alternate Names
      • Nitrotyrosine AlexaFluor® 647 Conjugate
      Background InformationNitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species. Proteins are often targets of reactive species and detection of oxidatively modified proteins is often used as an index of oxidative stress. Peroxynitrite is a strong oxidant formed by reaction of nitric oxide with superoxide. The reaction of proteins with peroxynitrite results in the oxidation of tryptophan and cysteine, as well as the nitration of tyrosine, formation of dityrosine, 2,4 dinitrophenylhydrazine-reactive carbonyls, and protein fragmentation. The formation of 3-nitrotyrosine represents a specific peroxynitrite-mediated protein modification that is different from modifications mediated by reactive oxygen species. Studies show that elevated nitrotyrosine levels are associated with inflammatory conditions, such as atherosclerotic plaques and rheumatoid arthritis.
      Product Information
      PresentationPurified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
      ApplicationThis Anti-Nitrotyrosine Antibody AlexaFluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of Nitrotyrosine.
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antibody (Cat. No. 06-284) is shown to be suitable also for Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation.
      Biological Information
      ImmunogenNitratred KLH.
      EpitopeNitrated Tyrosine
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityRecognizes proteins which are nitrated.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with all species.
      Antibody TypePolyclonal Antibody
      Purification MethodAffinity Purfied
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells treated with peroxynitrite (Cat. No. 20-107).

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Nitrotyrosine in HeLa cells treated with peroxynitrite (Cat. No. 20-107).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information