04-584 | Anti-PAK1 Antibody, rabbit monoclonal, clone EP656Y

04-584
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WB, ICC, IH(P)RbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-584
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PAK1 Antibody, rabbit monoclonal, clone EP656Y
      Alternate Names
      • p21-activated kinase 1
      Background Informationp21-activated kinase 1 (PAK1) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK1 participates in regulating the actin cytoskeleton, focal adhesion contacts, cell motility, apoptosis and transcription. It also promotes the disassembly of stress fibers (4-5). PAK1 also binds PIX (PAK-binding protein), COOL and adapter protein Nck (5).
      References
      Product Information
      FormatPurified
      PresentationBuffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-PAK1 Antibody, Rabbit, clone EP656Y is validated for use in IP, WB, IC, IH(P) for the detection of PAK1.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWB: 1:10,000-50,000
      IHC: 1: 250-500 (Paraffin-embedded human colon cancer)
      ICC: 1:250-500 (Hela cells)
      IP: 1:50
      Biological Information
      ImmunogenA synthetic peptide corresponding to residues in the human PAK1 protein.
      CloneEP656Y
      HostRabbit
      SpecificityRecognizes Human, Mouse, Rat PAK1.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NotePositive control: Hela cell lysate
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryPAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternative transcripts of this gene have been found, but their full-length natures have not yet been determined.
      Gene Symbol
      • PAK1
      • p65-PAK
      • MGC130001
      • Alpha-PAK
      • MGC130000
      • PAK-1
      • PAKalpha
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13153 # The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
      COFACTOR: Magnesium.
      SIZE: 545 amino acids; 60647 Da
      SUBUNIT: Homodimer in its autoinhibited state. Active as monomer. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1.
      SUBCELLULAR LOCATION: Cytoplasm. Cell junction, focal adhesion. Note=Recruited to focal adhesions upon activation.
      PTM: Autophosphorylated when activated by CDC42/p21 and RAC1.
      SIMILARITY: SwissProt: Q13153 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 CRIB domain. & Contains 1 protein kinase domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20 °C. Stable for 12 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PAK1 (Rabbit monoclonal) - 2302135 2302135
      Anti-PAK1 - 2494624 2494624
      Anti-PAK1 - DAM1413969 DAM1413969
      Anti-PAK1 - DAM1498519 DAM1498519
      Anti-PAK1 - DAM1632106 DAM1632106
      Anti-PAK1 - NG1829825 NG1829825
      Anti-PAK1 -2632516 2632516
      Anti-PAK1 -2691383 2691383

      References

      Reference overviewPub Med ID
      p21-activated kinases: three more join the Pak.
      Jaffer, Zahara M and Chernoff, Jonathan
      Int. J. Biochem. Cell Biol., 34: 713-7 (2002)  2002

      Show Abstract
      11950587 11950587
      p21-activated kinase 1 (Pak1) regulates cell motility in mammalian fibroblasts
      Sells, M A, et al
      J Cell Biol, 145:837-49 (1999)  1999

      10330410 10330410
      p21-activated protein kinase: a crucial component of morphological signaling?
      Daniels, R H and Bokoch, G M
      Trends Biochem Sci, 24:350-5 (1999)  1999

      10470034 10470034
      Inhibition of myosin light chain kinase by p21-activated kinase
      Sanders, L C, et al
      Science, 283:2083-5 (1999)  1999

      10092231 10092231
      A brain serine/threonine protein kinase activated by Cdc42 and Rac1
      Manser, E, et al
      Nature, 367:40-6 (1994)  1994

      8107774 8107774

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies