04-583 | Anti-PAK4 (NT) Antibody

04-583
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number04-583
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PAK4 (NT) Antibody
      Alternate Names
      • p21-activated kinase 4
      Background Informationp21-activated kinase 4 (PAK4) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK4 has been shown to regulate cytoskeletal organization and to protect cells from apoptosis induced by multiple stimuli, including serum withdrawal, tumor necrosis factor- (TNF) treatment, or UV irradiation (4). PAK4 expression is elevated in many cancer cell lines, and activated PAK4 is highly transforming, suggesting an important role in tumorigenesis (5).
      References
      Product Information
      FormatPurified
      PresentationBuffer: 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.
      Applications
      ApplicationThis Anti-PAK4 (N-Term) Antibody is validated for use in WB for the detection of PAK4 (N-Term).
      Key Applications
      • Western Blotting
      Application NotesWB: 1:1000
      Biological Information
      ImmunogenA synthetic peptide corresponding to residues in the N-terminus of human PAK4.
      HostRabbit
      SpecificityRecognizes Human, Mouse, Rat PAK4 (N-Term).
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat (positive control: SH-SY-5Y cell lysate)
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryPAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
      Gene Symbol
      • PAK4
      • PAK-4
      • KIAA1142
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O96013 # Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments.
      SIZE: 591 amino acids; 64072 Da
      SUBUNIT: Interacts with FGFR2 and GRB2 (By similarity). Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and weakly with RAC1.
      TISSUE SPECIFICITY: Highest expression in prostate, testis and colon.
      PTM: Autophosphorylated on serine residues when activated by CDC42/p21. & Phosphorylated on tyrosine residues upon stimulation of FGFR2 (By similarity).
      SIMILARITY: SwissProt: O96013 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 CRIB domain. & Contains 1 protein kinase domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20 °C. Stable for 12 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PAK4 (N-Term.) (Rabbit Polyclonal) - 2401304 2401304

      References

      Reference overviewPub Med ID
      p21-activated kinases: three more join the Pak.
      Jaffer, Zahara M and Chernoff, Jonathan
      Int. J. Biochem. Cell Biol., 34: 713-7 (2002)  2002

      Show Abstract
      11950587 11950587
      The serine/threonine kinase PAK4 prevents caspase activation and protects cells from apoptosis
      Gnesutta, N, et al
      J Biol Chem, 276:14414-9 (2001)  2001

      11278822 11278822
      Activated PAK4 regulates cell adhesion and anchorage-independent growth
      Qu, J, et al
      Mol Cell Biol, 21:3523-33 (2001)  2001

      11313478 11313478
      p21-activated protein kinase: a crucial component of morphological signaling?
      Daniels, R H and Bokoch, G M
      Trends Biochem Sci, 24:350-5 (1999)  1999

      10470034 10470034
      A brain serine/threonine protein kinase activated by Cdc42 and Rac1
      Manser, E, et al
      Nature, 367:40-6 (1994)  1994

      8107774 8107774

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies