CBL407 | Anti-PCNA Antibody, clone PC10

CBL407
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Inv, VrtELISA, FC, IP, WB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL407
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-PCNA Antibody, clone PC10
      Alternate Names
      • Proliferating Cell Nuclear Antigen
      • DNA Polymerase delta Processivity Factor
      Background InformationProliferating cell nuclear antigen (PCNA) is a 36 kDa molecule that is highly conserved between species. PCNA was first identified as the antigen for a subpopulation of autoantibodies in patients with systemic lupus erythematosus (Miyachi, 1978; Takasaki, 1984; Ogata, 1987). It has since been determined that PCNA serves as a co-factor for DNA polymerase delta in S-phase as well as during DNA synthesis associated with mechanisms involved in DNA damage repair (Tan, 1987; Bravo, 1987). The temporal specificity of PCNA expression makes it an ideal marker for cell proliferation. PCNA begins to accumulate during the G1 phase of the cell cycle, is most abundant during the S phase, and declines during the G2/M phase (Kurki, 1988). Since the half-life of PCNA exceeds 20 hours, it may be possible to detect the protein in non-cycling cells.
      References
      Product Information
      FormatPurified
      Control
      • POSITIVE CONTROL: Tonsil or reactive lymph node.
      PresentationThe monoclonal is presented at a concentration of 100μg/1mL in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationAnti-PCNA Antibody, clone PC10 is a mouse monoclonal antibody for detection of PCNA also known as Proliferating Cell Nuclear Antigen, DNA Polymerase delta Processivity Factor & has been validated in ELISA, FC, IP, WB,IHC(P).
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot: 1:250-1:1000. Nuclear extracts are preferred over whole cell protein extracts.

      Immunohistochemistry: 1:20-1:200. Recommended for paraffin embedded tissue sections only. May be used on material fixed in a wide range of fixatives including formalin (buffered and unbuffered), methacarn and Bouin's reagent. The time of fixation can markedly affect the intensity of PCNA immunoreactivity. Staining is reduced (and may be abolished) if sections are baked onto glass slides. Air-drying overnight on poly-L-lysine coated slides is recommended. 60 minute incubation at 25°C with standard ABC technique is recommended.

      Immunoprecipitation

      Indirect Flow Cytometry

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRat PCNA made in the protein A vector pR1T2T.
      ClonePC10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityClone PC10 recognizes PCNA from all vertebrate and insect species tested and has been used to identify transformed cells (Kurki, 1988), proliferating cells in solid tumors (Smetana, 1983), and blast cells in leukemia patients (Takasaki, 1984). Immunohistochemistry with clone PC10 has been used to study the expression of PCNA in paraffin sections of normal tissues and lymphoid neoplasms (Hall, 1990). In proliferating cells, PCNA staining pattern is predominantly nuclear. By western blot, the antibody detects a polypeptide migrating at 36 kDa with an isoelectric point of 4.8.
      IsotypeIgG2a
      Species Reactivity
      • Invertebrates
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
      Gene Symbol
      • PCNA
      • Cyclin
      • cyclin
      • MGC8367
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P12004 # This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
      SIZE: 261 amino acids; 28769 Da
      SUBUNIT: Homotrimer. Interacts with KCTD10 (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH, POLK, DNMT1, ERCC5/XPG, FEN1, CDC6, APEX2 and POLDIP2. Interacts with EXO1 and SHPRH.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Upon methyl methanesulfonate-induced DNA damage, mono- ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligase SHPRH, whih is required for DNA repair.
      DISEASE:SwissProt: P12004 # Antibodies are present in sera from patients with systemic lupus erythematosus.
      SIMILARITY: SwissProt: P12004 ## Belongs to the PCNA family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C. Avoid repeated freeze/ thawing.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN (PCNA) MONOCLONAL ANTIBODY 2988111
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN (PCNA) MONOCLONAL ANTIBODY 2926063
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN (PCNA) MONOCLONAL ANTIBODY - 2022040 2022040
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN (PCNA) MONOCLONAL ANTIBODY - 2050231 2050231
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN (PCNA) MONOCLONAL ANTIBODY - 2290960 2290960
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN (PCNA) MONOCLONAL ANTIBODY -2488943 2488943

      References

      Reference overviewPub Med ID
      BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions.
      Kretzschmar, K; Cottle, DL; Donati, G; Chiang, MF; Quist, SR; Gollnick, HP; Natsuga, K; Lin, KI; Watt, FM
      Stem cell reports  3  620-33  2014

      Show Abstract
      25358790 25358790
      Genomic organization, transcriptomic analysis, and functional characterization of avian α- and β-keratins in diverse feather forms.
      Ng, CS; Wu, P; Fan, WL; Yan, J; Chen, CK; Lai, YT; Wu, SM; Mao, CT; Chen, JJ; Lu, MY; Ho, MR; Widelitz, RB; Chen, CF; Chuong, CM; Li, WH
      Genome biology and evolution  6  2258-73  2014

      Show Abstract
      25152353 25152353
      Regeneration of reptilian scales after wounding: neogenesis, regional difference, and molecular modules.
      Wu, P; Alibardi, L; Chuong, CM
      Regeneration (Oxford, England)  1  15-26  2014

      Show Abstract
      24665362 24665362
      Efficient parvovirus replication requires CRL4Cdt2-targeted depletion of p21 to prevent its inhibitory interaction with PCNA.
      Adeyemi, RO; Fuller, MS; Pintel, DJ
      PLoS pathogens  10  e1004055  2014

      Show Abstract
      24699724 24699724
      Disrupted ectodermal organ morphogenesis in mice with a conditional histone deacetylase 1, 2 deletion in the epidermis.
      Hughes, MW; Jiang, TX; Lin, SJ; Leung, Y; Kobielak, K; Widelitz, RB; Chuong, CM
      The Journal of investigative dermatology  134  24-32  2014

      Show Abstract
      23792463 23792463
      Disruption of Tacc3 function leads to in vivo tumor regression.
      R Yao,Y Natsume,Y Saiki,H Shioya,K Takeuchi,T Yamori,H Toki,I Aoki,T Saga,T Noda
      Oncogene  31  2012

      Show Abstract
      21685933 21685933
      The chicken frizzle feather is due to an α-keratin (KRT75) mutation that causes a defective rachis.
      Ng, CS; Wu, P; Foley, J; Foley, A; McDonald, ML; Juan, WT; Huang, CJ; Lai, YT; Lo, WS; Chen, CF; Leal, SM; Zhang, H; Widelitz, RB; Patel, PI; Li, WH; Chuong, CM
      PLoS genetics  8  e1002748  2012

      Show Abstract
      22829773 22829773
      Epithelial Notch signaling regulates interstitial fibrosis development in the kidneys of mice and humans.
      Bielesz, B; Sirin, Y; Si, H; Niranjan, T; Gruenwald, A; Ahn, S; Kato, H; Pullman, J; Gessler, M; Haase, VH; Susztak, K
      The Journal of clinical investigation  120  4040-54  2010

      Show Abstract
      20978353 20978353
      PCNA/cyclin expression and BrdU uptake define different subpopulations in different cell lines.
      Coltrera, M D and Gown, A M
      J. Histochem. Cytochem., 39: 23-30 (1991)  1991

      Show Abstract
      1670579 1670579
      Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.
      Waseem, N H and Lane, D P
      J. Cell. Sci., 96 ( Pt 1): 121-9 (1990)  1990

      Show Abstract
      1695635 1695635

      Data Sheet

      Title
      Anti-PCNA, clone PC10 - Data Sheet

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies