07-1437 | Anti-PDGF-BB Antibody

07-1437
100 µL  
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      Overview

      Replacement Information
      Replacement Information07-1437 is a recommended replacement for AB1487P.

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ELISA, NEUTRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1437
      ReplacesAB1487P
      DescriptionAnti-PDGF-BB Antibody
      Alternate Names
      • Platelet-derived growth factor B chain precursor
      • Platelet-derived growth factor beta polypeptide
      • Platelet-derived growth factor, beta polypeptide (oncogene SIS)
      • oncogene SIS
      • Platelet-derived growth factor
      • Platelet-derived growth factor beta
      • Platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
      • Platelet-derived growth factor, B chain
      • V-sis platelet-derived growth factor beta polypeptide (simian sarcoma viral oncogene homolog)
      Background InformationPlatelet-Derived Growth Factor (PDGF) is a dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB). Its receptors are transmembrane receptor tyrosine kinases, PDGF Receptors type A and B. The type A PDGF receptor binds all three PDGFs with high affinity, whereas the type B PDGF receptor is selective for PDGF-BB. PDGF signals as a growth and survival factor, as it is a potent activator of the PI3 Kinase pathway. PDGF signaling appears to play an essential role in the development of tumors of glial origin.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal IgG lyophilized from 0.5X PBS, pH 7.4. Reconstitute with sterile distilled water.
      Applications
      ApplicationAnti-PDGF-BB Antibody detects level of PDGF-BB & has been published & validated for use in WB, ELISA, NEUT.
      Key Applications
      • Western Blotting
      • ELISA
      • Neutralizing
      Application NotesELISA: 0.5 μg/mL dilution of a previous lot will allow detection (in direct ELISA) of
      0.2-0.4 ng/well when human PDGF-BB is used to coat the plate.

      Neutralization: To yield ½ maximal inhibition (ND50) of the biological activity of hPDGF-BB (2.5
      ng/mL), a concentration of 0.03-0.04 μg/mL of a previous lot of this antibody was required.

      Immunohistochemistry(paraffin): Anti-IGF-1R, representative staining-pattern and morphology on ductal breast carcinoma. Tissue was pretreated with EDTA, pH 8.0 for Epitope retrieval. A previous lot of this antibody was diluted to 1:200 (RT incubation), using IHC-Select® Detection with HRP-DAB.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHighly purified (>98%) recombinant human PDGF-BB
      HostRabbit
      SpecificityReacts with human PDGF-BB.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene. [provided by RefSeq].
      Gene Symbol
      • PDGFB
      • FLJ12858
      • PDGF2
      • c-sis
      • PDGF-2
      • SSV
      • SIS
      • Becaplermin
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
      SUBUNIT: Antiparallel disulfide-linked dimer of non-identical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. Interacts with XLKD1 (By similarity).
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
      DISEASE: A chromosomal aberration involving PDGFB is a cause of dermatofibrosarcoma protuberans (DFSP) [MIM:607907]. Translocation t(17;22)(q22;q13) with COLA1. DFSP is an uncommon, locally aggressive, but rarely metastasizing tumor of the deep dermis and subcutaneous tissue. It typically occurs during early or middle adult life and is most frequently located on the trunk and proximal extremities.
      PHARMACEUTICAL: Available under the name Regranex (Ortho-McNeil). Used to promote healing in diabetic neuropathic foot ulcers.
      MISCELLANEOUS: A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor.
      SIMILARITY: Belongs to the PDGF/VEGF growth factor family.

      Molecular Weight28 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Huvec lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected PDGF-BB on 10 μg of Huvec lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material desiccated below 0°C. After reconstitution maintain at -20°C in convenient undiluted aliquots for up to 6 months from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PDGF-BB - 2392211 2392211
      Anti-PDGF-BB - 2398832 2398832
      Anti-PDGF-BB - 1946579 1946579
      Anti-PDGF-BB - 2198974 2198974
      Anti-PDGF-BB - 2211045 2211045
      Anti-PDGF-BB - 2262630 2262630
      Anti-PDGF-BB - 2283807 2283807
      Anti-PDGF-BB - 2318075 2318075
      Anti-PDGF-BB - DAM1583133 DAM1583133
      Anti-PDGF-BB - JBC1787304 JBC1787304

      References

      Reference overviewPub Med ID
      Safety and tolerability of intracerebroventricular PDGF-BB in Parkinson's disease patients.
      Paul, G; Zachrisson, O; Varrone, A; Almqvist, P; Jerling, M; Lind, G; Rehncrona, S; Linderoth, B; Bjartmarz, H; Shafer, LL; Coffey, R; Svensson, M; Mercer, KJ; Forsberg, A; Halldin, C; Svenningsson, P; Widner, H; Frisén, J; Pålhagen, S; Haegerstrand, A
      The Journal of clinical investigation  125  1339-46  2015

      Show Abstract
      25689258 25689258

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies