04-825 | Anti-PDGFRβ Antibody, clone 4A56, rabbit monoclonal

04-825
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HIP, WBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-825
      Replaces05-825
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PDGFRβ Antibody, clone 4A56, rabbit monoclonal
      References
      Product Information
      FormatCulture Supernatant
      Control
      • RIPA lysates from mouse NIH/3T3, 3T3/A31, or human HFF cells
      PresentationCultured supernantant in 0.05% sodium azide
      Applications
      ApplicationThis Anti-PDGFRβ Antibody, clone 4A56 is validated for use in IP, WB for the detection of PDGFRβ.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesWesterm Blot Analysis:
      A 1:2,000 dilution of this lot detected PDGFRβ in RIPA lysates from mouse NIH/3T3, 3T3/A31, and human HFF cells.

      Immunoprecipitation: 4 μg of a previous lot immunoprecipitated PDGFRβ from 500 μg of 3T3/A31 RIPA lysate.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 710-718 of human PDGF Receptor β. An N-terminus cysteine was added to facilitate conjugation. This sequence corresponds to amino acids 709-717 of mouse PDGF Receptor β.
      Clone4A56
      HostRabbit
      SpecificityPDGFRβ, does not cross-react with EGF receptor
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
      Gene Symbol
      • PDGF-R-beta
      • CD140B
      • JTK12
      • PDGFR
      • CD140b
      • PDGFR1
      • PDGFRB
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09619 # Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
      SIZE: 1106 amino acids; 123968 Da
      SUBUNIT: Homodimer, and heterodimer with PDGFRA. Interacts with APS. The autophosphorylated form interacts directly with SHB and with PIK3C2B, maybe indirectly.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DISEASE: SwissProt: P09619 # A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML). & A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia. & A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1. & A chromosomal aberration involving PDGFRB is a cause in many instances of chronic myeloproliferative disorder with eosinophilia (MPE) [MIM:131440]. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein. & A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP- PDGFRB fusion protein.
      SIMILARITY: SwissProt: P09619 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
      Molecular Weight190kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from mouse NIH/3T3, 3T3/A31, or human HFF cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies