05-1135-25UG | Anti-PDGFR-b Antibody, clone CH-3 (mouse monoclonal)

New05-1135-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ELISA, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1135-25UG
      DescriptionAnti-PDGFR-b Antibody, clone CH-3 (mouse monoclonal)
      Alternate Names
      • Platelet Derived Growth Factor Receptor Beta
      • PDGF-R-beta
      • PDGFR-beta
      • Beta platelet-derived growth factor receptor
      • Beta-type platelet-derived growth factor receptor
      • CD140 antigen-like family member B
      • Platelet-derived growth factor receptor 1
      • PDGFR-1
      • CD140b
      Background InformationPDGFR (Platelet-Derived Growth Factor Receptor) is a receptor tyrosine kinase for the PDGF family of growth factors. PDGF homo and hetero- dimmers (dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB)) bind to the two structurally related a and b PDGF Receptors (PDGFRa and PDGFRb). The type A PDGF Receptor binds all three PDGFs with high affinity, whereas the type B PDGF Receptor is selective for PDGF-BB. This binding results in the dimerization of the receptor and the subsequent kinase activation of the receptor tyrosine kinase (RTK) and its autophosphorylation. These phosphorylations allow for various proteins, such as PI3 Kinase, PLCg, and Grb2, to bind through their SH2 domains and transduce signals into the cell. The activation of PDGFFR triggers the intracellular loop and activates Ras, Erk1/2, and ROS. PDGFb has been implicated in embryonic development, angiogenesis, and development of tumors of glial origin.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate
      PresentationPurified mouse monoclonal IgG1 in buffer containing 20 mM sodium phosphate, 250 mM NaCl, 0.1% NaN3, pH. 7.6
      Applications
      ApplicationThis Anti-PDGFR Antibody, clone CH-3 is validated for use in WB, ELISA, IH(P) for the detection of PDGFR.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunohistochemistry (Paraffin)
      Application NotesELISA: A previous lot of this antibody was used in ELISA.

      Immunohistochemistry(paraffin): Representative images from a previous lot.
      Optimal Staining of PDGF-A With Citrate pH 6.0 Epitope Retrieval: Rat Brain
      Biological Information
      ImmunogenRecombinant Platelet-Derived Growth Factor Receptor
      CloneCH-3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman and mouse. Expected to react with rat based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight211 amino acids; 24043 Da
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Jurkat lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected PDGFR on 10 μg of Jurkat lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at + 2-8 ° C in undiluted aliquots for up to 1 year from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications