06-1379 | Anti-PDX1 (rabbit) Antibody

06-1379
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, IHCRbSerumPolyclonal Antibody
      Description
      Catalogue Number06-1379
      DescriptionAnti-PDX1 (rabbit) Antibody
      Alternate Names
      • pancreatic and duodenal homeobox 1
      • pancreatic-duodenal homeobox factor 1
      • somatostatin transcription factor 1
      • insulin promoter factor 1, homeodomain transcription factor
      • Insulin promoter factor 1
      • Somatostatin-transactivating factor 1
      • Insulin upstream factor 1
      • Glucose-sensitive factor
      • Islet/duodenum homeobox-1
      Background InformationTranscription factor PDX1, also known as pancreatic and duodenal homeobox 1, activates expression of the insulin and somatostatin genes. This protein is a key regulator of islet peptide hormone expression and also plays an essential role in pancreatic development. It has been shown to interact with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). This protein also interacts with the methyltransferase SETD7. Mutations in this gene may be involved in several disorders of the pancreas or in diabetes mellitus.
      References
      Product Information
      FormatSerum
      Control
      • Mouse pancreatic β T-cell lysate
      PresentationUnpurified rabbit polyclonal containing 0.05% sodium azide.
      Applications
      ApplicationAnti-PDX1 (rabbit) Antibody is a Rabbit Polyclonal Antibody for detection of PDX1 also known as pancreatic & duodenal homeobox 1, somatostatin transcription factor 1, Insulin promoter factor 1 & has been validated in WB & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: 1:500 dilution from a representative lot detected PDX1 in normal human pancreas tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the N-terminus of mouse PDX1.
      EpitopeN-terminus
      HostRabbit
      SpecificityThis antibody recognizes the N-terminus of PDX1.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GSF
      • IDX-1
      • STF-1
      • IUF1
      • PDX-1
      • MODY4
      • IPF1
      • IUF-1
      • IPF-1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.

      SUBUNIT STRUCTURE: Interacts with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). Interacts with the methyltransferase SETD7 By similarity. Interacts with SPOP.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).

      DEVELOPMENTAL STAGE: At 8.5 dpc, detected in the gut epithelium from which the pancreatic buds are formed. Transient expression in pancreatic ducts, endocrine and acinar cells. Down-regulated around 10.5 dpc when expression becomes restricted to differentiated beta-cells.

      DOMAIN: The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter (By Similarity).

      The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y) (By Similarity).

      PTM: Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.

      SEQUENCE SIMILARITIES: Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.

      Contains 1 homeobox DNA-binding domain.
      Molecular Weight~ 39 kDa Observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse pancreatic β T-cell lysate.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected PDX1 on 10 µg of mouse pancreatic β T-cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PDX1 (rabbit) 2470936
      Anti-PDX1 (rabbit) - 2128272 2128272
      Anti-PDX1 (rabbit) - 2424763 2424763
      Anti-PDX1 (rabbit) - 2207241 2207241
      Anti-PDX1 (rabbit) - NG1827446 NG1827446
      Anti-PDX1 (rabbit) - NG1866946 NG1866946
      Anti-PDX1 (rabbit) - NG1928927 NG1928927
      Anti-PDX1 (rabbit) - NRG1736369 NRG1736369
      Anti-PDX1 (rabbit) -2521195 2521195
      Anti-PDX1 (rabbit) -2853595 2853595

      References

      Reference overviewApplicationPub Med ID
      Deletion of retinoic acid receptor β (RARβ) impairs pancreatic endocrine differentiation.
      Pérez, RJ; Benoit, YD; Gudas, LJ
      Experimental cell research  319  2196-204  2013

      Show Abstract
      Immunofluorescence23756134 23756134

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies