CBL468P | Anti-PECAM-1 Antibody, clone HC1/6, Phycoerythrin conjugated

CBL468P
100 assays  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMPhycoerythrinMonoclonal Antibody
      Description
      Catalogue NumberCBL468P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-PECAM-1 Antibody, clone HC1/6, Phycoerythrin conjugated
      Alternate Names
      • CD31
      References
      Product Information
      FormatPhycoerythrin
      PresentationThe conjugate is supplied as a 100 test vial in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. For flow cytometry we recommend using 10μL of conjugate per test.
      Applications
      ApplicationAnti-PECAM-1 Antibody, clone HC1/6, Phycoerythrin conjugated detects level of PECAM-1 & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Application NotesStudies of the role of CD31 in leucocyte migration into inflammatory sites and in the process of angiogenesis

      Suitable for direct staining in flow cytometry

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPMA - treated U937 cells
      CloneHC1/6
      HostMouse
      SpecificityThe antibody reacts with a 130-140 kDa transmembranous glycoprotein that is expressed widely amongst cells of the vascular compartment. CD31 is expressed on platelets, cultured endothelial cells, monocytes, normal tissue macrophages in tonsil, lung and kidney as well as in skin biopsies from conditions such as sarcoidosis and lepromatous leprosy.

      FUSION PARTNER: P3-x63Ag8.653 myeloma cell line
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PECAM1
      • EndoCAM
      • GPIIA
      • PECAM-1
      • CD31
      • CD31/EndoCAM
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16284 # This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.
      SIZE: 738 amino acids; 82536 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.
      PTM: Phosphorylated on Ser and Tyr residues after cellular activation.
      SIMILARITY: SwissProt: P16284 ## Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Molecular and functional aspects of PECAM-1/CD31
      DeLisser, H. M. et al.
      Immunol. Today, 15:490-495 (1994)  1994

      7945775 7945775
      Molecular characterization and functional analysis of the leukocyte surface protein CD31
      Stockinger, H. et al.
      J. Immunol., 145:3889-3897 (1990)  1990

      1700999 1700999
      Characterisation of a novel myeloid antigen regulated during differentiation of monocytic cells
      Carbanas, C. et al.
      Eur. J. Immunol., 19:1373-1378 (1989)  1989

      2506060 2506060

      Data Sheet

      Title
      Anti-PECAM-1, Clone HC1/6, Phycoerythrin Conjugated - Data Sheet

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      Alternative Format

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies