04-1074 | Anti-PECAM-1 [CD31] Antibody, clone EP3095, rabbit monoclonal

04-1074
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P), FCRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1074
      DescriptionAnti-PECAM-1 [CD31] Antibody, clone EP3095, rabbit monoclonal
      Alternate Names
      • CD31 antigen
      • PECAM-1
      • CD31/EndoCAM, adhesion molecule
      Background InformationCD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1) is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction.
      References
      Product Information
      FormatUnpurified
      Control
      • THP-1 and Jurkat cell lysates
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-PECAM-1 [CD31] Antibody, clone EP3095, Rabbit detects level of PECAM-1 [CD31] & has been published & validated for use in WB, IH(P), FC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: 1:250 - 500 dilution from a previous lot detected PECAM1 in human uterus and tonsil tissue.

      Flow Cytometry: 1:70 dilution from a previous lot was used on FC.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues on human PECAM-1.
      CloneEP3095
      HostRabbit
      SpecificityThis antibody recognizes PECAM1.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CD31
      • CD31/EndoCAM
      • EndoCAM
      • GPIIA
      • PECAM-1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.

      PTM: Phosphorylated on Ser and Tyr residues after cellular activation.

      SEQUENCE SIMILARITIES: Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

      Molecular Weight130 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on THP-1 and Jurkat cell lysates.

      Western Blot Analysis: 1:5,000 - 20,000 dilution of this antibody was used to detect PECAM1 in THP-1 and Jurkat cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PECAM-1 [CD31], clone EP3095 2475599
      Anti-PECAM-1 [CD31], clone EP3095 - 2492092 2492092
      Anti-PECAM-1 [CD31], clone EP3095, Rabbit Monoclonal - 2135141 2135141
      Anti-PECAM-1 [CD31], clone EP3095, Rabbit Monoclonal - 2398828 2398828
      Anti-PECAM-1 [CD31], clone EP3095, Rabbit Monoclonal - 2060990 2060990
      Anti-PECAM-1 [CD31], clone EP3095, Rabbit Monoclonal - 2208790 2208790
      Anti-PECAM-1 [CD31], clone EP3095, Rabbit Monoclonal - 2291702 2291702
      Anti-PECAM-1 [CD31], clone EP3095, Rabbit Monoclonal - 2529869 2529869
      Anti-PECAM-1 [CD31], clone EP3095, Rabbit Monoclonal - JBC1771176 JBC1771176
      Anti-PECAM-1 [CD31], clone EP3095, Rabbit Monoclonal - JBC1791514 JBC1791514

      References

      Reference overviewApplicationPub Med ID
      Adenosine triphosphate-binding cassette transporter G2 expression in endometriosis and in endometrium from patients with and without endometriosis.
      Matsuzaki, Sachiko and Darcha, Claude
      Fertil. Steril., 98: 1512-1520.e3 (2012)  2012

      Show Abstract
      22925688 22925688
      Functional redundancy of SWI/SNF catalytic subunits in maintaining vascular endothelial cells in the adult heart.
      Willis, MS; Homeister, JW; Rosson, GB; Annayev, Y; Holley, D; Holly, SP; Madden, VJ; Godfrey, V; Parise, LV; Bultman, SJ
      Circulation research  111  e111-22  2012

      Show Abstract
      22740088 22740088
      In vitro model of vascularized bone: synergizing vascular development and osteogenesis.
      Correia, C; Grayson, WL; Park, M; Hutton, D; Zhou, B; Guo, XE; Niklason, L; Sousa, RA; Reis, RL; Vunjak-Novakovic, G
      PloS one  6  e28352  2011

      Show Abstract Full Text Article
      Immunohistochemistry22164277 22164277

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies