05-1327 | Anti-PHD2 Antibody, clone 76a

05-1327
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1327
      DescriptionAnti-PHD2 Antibody, clone 76a
      Alternate Names
      • EGL nine (C.elegans) homolog 1
      • HIF prolyl hydroxylase 2
      • HIF-prolyl hydroxylase 2
      • Hypoxia-inducible factor prolyl hydroxylase 2
      • Prolyl hydroxylase domain-containing protein 2
      • egl nine homolog 1
      • egl nine homolog 1 (C. elegans)
      • egl nine-like protein 1
      Background InformationPHD2 (Prolyl hydroxylase domain-containing protein 2), also known as Hypoxia-inducible factor prolyl hydroxylase 2 (HIF-PH2 or HPH-2), is one of 4 PHD (PHD1-4) proteins which function as prolyl hydroxylases to effect oxygen homeostasis via hypoxia-inducible factor (HIF). This transcriptional complex is composed of an alpha-beta heterodimer that mediates a broad range of cellular and systemic responses to hypoxia. The PHD proteins hydroxylate HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. The PHD2 isoform is the main down-regulator of HIFs for normal oxygen levels and mild hypoxia. When oxygen levels are normal, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha. This post-translational modification targets this protein for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
      References
      Product Information
      FormatPurified
      Control
      • MCF-7 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-PHD2 Antibody, clone 76a (mouse monoclonal antibody) validated in WB to detect PHD2 also known as EGL nine (C.elegans) homolog 1, HIF prolyl hydroxylase 2, Hypoxia-inducible factor prolyl hydroxylase 2.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenRecombinant protein corresponding to human PHD2.
      EpitopeUnknown
      Clone76a
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes PHD2.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGLN1
      • C1orf12
      • ECYT3
      • HIF-PH2
      • HIFPH2
      • HPH-2
      • HPH2
      • PHD2
      • SM-20
      • SM20
      • ZMYND6
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.

      CATALYTIC ACTIVITY: An HIF alpha chain L-proline + 2-oxoglutarate + O2 = An HIF alpha chain trans-4-hydroxy-L-proline + succinate + CO2.

      COFACTOR: Binds 1 Fe2+ ion per subunit.

      Ascorbate.

      ENZYME REGULATION: Following exposure to hypoxia, activated in HeLa cells but not in cardiovascular cells. Seems to be inhibited by ING4.

      SUBUNIT STRUCTURE: Monomer. Interacts with ING4.

      TISSUE SPECIFICITY: According to Ref.1 widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to Ref.8 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle.

      INVOLVEMENT IN DISEASE: Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3) [MIM:609820]. ECYT3 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels.

      SEQUENCE SIMILARITIES: Contains 1 MYND-type zinc finger.

      Contains 1 PKHD (prolyl/lysyl hydroxylase) domain.

      CAUTION: It was previously reported that this protein was the ortholog of rat SM-20. However, EGLN3 is now considered the true ortholog of rat SM-20 since it shows substantially greater similarity.

      SEQUENCE CAUTION: The sequence AAK07536.1 differs from that shown. Reason: Frameshift at position 239.
      Molecular Weight ~ 46 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in MCF-7 cell lysate.

      Western Blot Analysis: 2 µg/ml of this antibody detected PHD2 on 10 µg of MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PHD2, clone 76a - 2216890 2216890
      Anti-PHD2, clone 76a - NG1915719 NG1915719
      Anti-PHD2, clone 76a - NRG1675268 NRG1675268
      Anti-PHD2, clone 76a -2524055 2524055
      Anti-PHD2, clone 76a -2834696 2834696
      Anti-PHD2, clone 76a -2888836 2888836
      Anti-PHD2, clone 76a Monoclonal Antibody 2895643

      References

      Reference overviewPub Med ID
      Photoactivable peptides for identifying enzyme-substrate and protein-protein interactions.
      Rotili, Dante, et al.
      Chem. Commun. (Camb.), 47: 1488-90 (2011)  2011

      Show Abstract
      21152646 21152646
      Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor.
      Appelhoff, Rebecca J, et al.
      J. Biol. Chem., 279: 38458-65 (2004)  2004

      Show Abstract
      15247232 15247232