04-400 | Anti-PI3 Kinase Antibody, p110β, rabbit monoclonal

04-400
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IP, WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-400
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PI3 Kinase Antibody, p110β, rabbit monoclonal
      Alternate Names
      • PI3-kinase p110 subunit beta
      • PtdIns-3-kinase p110
      • phosphatidylinositol 3-kinase, catalytic, beta polypeptide
      • phosphoinositide-3-kinase, catalytic, beta polypeptide
      Background InformationPI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate
      PresentationPurified rabbit monoclonal IgG in buffer containing 0.03% BSA, 30 mM Tris-Glycine (pH 7.4), 0.09 M NaCl, 0.006% sodium azide as a preservative in a solution of 40% glycerol
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-PI3 Kinase Antibody, p110β, Rabbit is validated for use in FC, IP, WB for the detection of PI3 Kinase.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      A 1:50 dilution of a previous lot of this antibody immunoprecipitated PI3 Kinase, p110β using protein G:agarose beads.

      Flow Cytometry:
      A 1:40 of a previous lot of this antibody stained cells for intracellular staining for flow Cytometry analysis.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to the N-terminal region of human PI3 Kinase, p110β
      EpitopeN-terminus
      CloneY384
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes a region in the N-terminus domain of human PI3 Kinase, p110β. Non specific band detected at MW 55 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human. Reactivity with other species has not been confirmed.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryPhosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM]
      Gene Symbol
      • PIK3CB
      • PI3Kbeta
      • MGC133043
      • PIK3C1
      • DKFZp779K1237
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42338 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
      SIZE: 1070 amino acids; 122762 Da
      SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit.
      TISSUE SPECIFICITY: Expressed ubiquitously.
      SIMILARITY: SwissProt: P42338 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.
      Molecular Weight110 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on jurkat cell lysate.

      Western Blot Analysis:
      A 1:500 dilution of this lot detected endogenous PI3 Kinase, p110β in lysate from Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PI3 Kinase, p110 Rabbit Monoclonal - DAM1514039 DAM1514039
      Anti-PI3 Kinase, p110 Rabbit Monoclonal - DAM1524107 DAM1524107
      Anti-PI3 Kinase, p110 Rabbit Monoclonal - NG1899907 NG1899907
      Anti-PI3 Kinase, p110#946; Rabbit Monoclonal - 1985140 1985140
      Anti-PI3 Kinase, p110b, Rabbit Monoclonal - DAM1462584 DAM1462584
      Anti-PI3 Kinase, p110b, Rabbit Monoclonal - DAM1514039 DAM1514039
      Anti-PI3 Kinase, p110beta;, rabbit monoclonal - 0611045806 0611045806
      Anti-PI3 Kinase, p110beta;, rabbit monoclonal - DAM1408854 DAM1408854
      Anti-PI3 Kinase, p110beta;, rabbit monoclonal - DAM1409495 DAM1409495
      Anti-PI3 Kinase, p110beta;, rabbit monoclonal - JBC1369987 JBC1369987

      References

      Reference overviewPub Med ID
      Increased insulin sensitivity in mice lacking p85beta subunit of phosphoinositide 3-kinase
      Ueki, Kohjiro, et al
      Proc Natl Acad Sci USA, 99:419-24 (2002)  2002

      11752399 11752399
      A function for phosphoinositide 3-kinase beta lipid products in coupling beta gamma to Ras activation in response to lysophosphatidic acid
      Yart, Armelle, et al
      J Biol Chem, 277:21167-78 (2002)  2002

      11916960 11916960

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies