04-401 | Anti-PI3 Kinase Antibody, p110δ rabbit monoclonal

04-401
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIF, IP, WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-401
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PI3 Kinase Antibody, p110δ rabbit monoclonal
      Alternate Names
      • PI3-kinase p110 subunit delta
      • PtdIns-3-kinase p110
      • phosphatidylinositol 3-kinase, catalytic, delta polypeptide
      • phosphoinositide-3-kinase C
      • phosphoinositide-3-kinase, catalytic, delta polypeptide
      Background InformationPI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate
      PresentationPurified rabbit monoclonal IgG in buffer containing 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-PI3 Kinase Antibody, p110δ Rabbit is an antibody against PI3 Kinase for use in IF, IP & WB.
      Key Applications
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      A 1:40 dilution of a prevoius lot of this antibody immunoprecipitated PI3 Kinase, p110δ using protein G:agarose beads.

      Immunfluorescence Staining:
      A 1:250 dilution of a previous lot of this antibody was used for Immunfluorescence staining of paraformaldehyde fixed HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to human PI3 Kinase, p110δ
      HostRabbit
      SpecificityThis antibody recognizes human PI3 Kinase, p110δ.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human. Not believed to cross-react with mouse or rat. Reactivity with other species has not been confirmed.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryPhosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. See MIM 602838. The class I PI3Ks display a broad phosphoinositide lipid substrate specificity and include p110-alpha (MIM 171834), p110-beta (MIM 602925), and p110-gamma (MIM 601232). p110-alpha and p110-beta interact with SH2/SH3-domain-containing p85 adaptor proteins (see MIM 171833) and with GTP-bound Ras.[supplied by OMIM]
      Gene Symbol
      • PIK3CD
      • p110d
      • p110delta
      • PI3K
      • p110D
      Purification MethodPurified
      UniProt Number
      UniProt SummarySIZE: 1044 amino acids; 119479 Da
      SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Interacts with ERAS (By similarity).
      TISSUE SPECIFICITY: Expressed predominantly in leukocytes.
      PTM: Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
      SIMILARITY: SwissProt: O00329 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.
      Molecular Weight110 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Jurkat cell lysate.

      Western Blot Analysis:
      A 1:500 dilution of this lot detected endogenous PI3 Kinase, p110δ in lysate from Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PI3 Kinase, p110 Rabbit Monoclonal - 2092608 2092608
      Anti-PI3 Kinase, p110 Rabbit Monoclonal - 2199582 2199582
      Anti-PI3 Kinase, p110 Rabbit Monoclonal - 2309017 2309017
      Anti-PI3 Kinase, p110 Rabbit Monoclonal - 2330487 2330487
      Anti-PI3 Kinase, p110 Rabbit Monoclonal - DAM1413956 DAM1413956
      Anti-PI3 Kinase, p110 Rabbit Monoclonal - JBC1368776 JBC1368776
      Anti-PI3 Kinase, p110#948; Rabbit Monoclonal - 2142145 2142145
      Anti-PI3 Kinase, p110delta;, rabbit monoclonal - JBC1368776 JBC1368776
      Anti-PI3 Kinase, p110δ Rabbit Monoclonal 2483150
      Anti-PI3 Kinase, p110δ Rabbit Monoclonal - 2382091 2382091

      References

      Reference overviewApplicationPub Med ID
      Dominant-activating germline mutations in the gene encoding the PI(3)K catalytic subunit p110δ result in T cell senescence and human immunodeficiency.
      Lucas, CL; Kuehn, HS; Zhao, F; Niemela, JE; Deenick, EK; Palendira, U; Avery, DT; Moens, L; Cannons, JL; Biancalana, M; Stoddard, J; Ouyang, W; Frucht, DM; Rao, VK; Atkinson, TP; Agharahimi, A; Hussey, AA; Folio, LR; Olivier, KN; Fleisher, TA; Pittaluga, S; Holland, SM; Cohen, JI; Oliveira, JB; Tangye, SG; Schwartzberg, PL; Lenardo, MJ; Uzel, G
      Nature immunology  15  88-97  2014

      Show Abstract
      Western Blotting24165795 24165795
      Oncogenic transformation induced by the p110beta, -gamma, and -delta isoforms of class I phosphoinositide 3-kinase
      Kang, Sohye, et al
      Proc Natl Acad Sci USA, 103:1289-94 (2006)  2006

      16432180 16432180
      Essential role of phosphoinositide 3-kinase delta in neutrophil directional movement
      Sadhu, Chanchal, et al
      J Immunol, 170:2647-54 (2003)  2003

      12594293 12594293

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies