04-402 | Anti-PI3 Kinase Antibody, p110γ, rabbit monoclonal

04-402
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IP, WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-402
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PI3 Kinase Antibody, p110γ, rabbit monoclonal
      Alternate Names
      • PIK3CG
      • PI3CG
      • PI3K
      • PI3Kgamma
      • PIK3
      • 1-phosphatidylinositol 3-kinase
      • PI3-kinase
      • PTDINS-3-kinase
      • p110-gamma
      • phosphatidylinositol 3 kinase gamma
      Background InformationPI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate
      PresentationPurified rabbit monoclonal IgG in buffer containing 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect PI3 Kinase using this Anti-PI3 Kinase Antibody, p110γ validated for use in FC, IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      A 1:50 dilution of a previous lot of antibody immunoprecipitated PI3K, p110γ using protein G:agarose beads.

      Flow Cytometry:
      A 1:50 of a previous lot of this antibody stained cells for intracellular staining for flow Cytometry analysis.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to the N-terminus human PI3 Kinase, p110γ
      EpitopeN-terminus
      HostRabbit
      SpecificityThis antibody recognizes the N-terminus human PI3 Kinase, p110γ.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human. Reactivity with other species has not been confirmed.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias.
      Gene Symbol
      • PIK3CG
      • PI3CG
      • p110-gamma
      • PI3-kinase
      • PI3Kgamma
      • PTDINS-3-kinase
      • PIK3
      • PI3K
      • p120-PI3K
      Purification MethodPurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P48736 # 3-phosphorylates the cellular phosphoinositide PtdIns- 4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5- triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
      SIZE: 1102 amino acids; 126454 Da
      SUBUNIT: Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit.
      TISSUE SPECIFICITY: Pancreas, skeletal muscle, liver and heart.
      SIMILARITY: SwissProt: P48736 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.
      Molecular Weight110 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on jurkat cell lysate.

      Western Blot Analysis:
      A 1:1,000 dilution of the lot detected endogenous PI3 Kinase, p110γ in lysates from Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PI3 Kinase, p110 - 2043181 2043181
      Anti-PI3 Kinase, p110 - 2199584 2199584
      Anti-PI3 Kinase, p110 - 2370220 2370220
      Anti-PI3 Kinase, p110 - DAM1415014 DAM1415014
      Anti-PI3 Kinase, p110 - NG1827421 NG1827421
      Anti-PI3 Kinase, p110#947; - 2118922 2118922
      Anti-PI3 Kinase, p110g, rabbit monoclonal - DAM1644589 DAM1644589
      Anti-PI3 Kinase, p110gamma;, rabbit monoclonal - DAM1394903 DAM1394903
      Anti-PI3 Kinase, p110gamma;, rabbit monoclonal - DAM1411347 DAM1411347
      Anti-PI3 Kinase, p110gamma;, rabbit monoclonal - DAM1415014 DAM1415014

      References

      Reference overviewPub Med ID
      Oncogenic transformation induced by the p110beta, -gamma, and -delta isoforms of class I phosphoinositide 3-kinase
      Kang, Sohye, et al
      Proc Natl Acad Sci USA, 103:1289-94 (2006)  2006

      16432180 16432180
      Identification and characterization of the autophosphorylation sites of phosphoinositide 3-kinase isoforms beta and gamma
      Czupalla, Cornelia, et al
      J Biol Chem, 278:11536-45 (2003)  2003

      12502714 12502714

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies