04-403 | Anti-PI3 Kinase Antibody, p85α rabbit monoclonal

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, ICC, IF, IH(P), IP, WBRbPurifiedMonoclonal Antibody
      Catalogue Number04-403
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PI3 Kinase Antibody, p85α rabbit monoclonal
      Alternate Names
      • PI3-kinase p85 subunit alpha
      • PtdIns-3-kinase p85-alpha
      • phosphatidylinositol 3-kinase, regulatory subunit,polypeptide 1 (p85
      • phosphatidylinositol 3-kinase, regulatory, 1
      • phosphatidylinositol 3-kinase-associated p-85 alpha
      • phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
      • phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha)
      • phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85
      Background InformationPI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
      Product Information
      • Lysates from A431, MCF-7 or Raji cells
      PresentationPurified rabbit monoclonal IgG in buffer containing 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect PI3 Kinase using this Anti-PI3 Kinase Antibody, p85α Rabbit validated for use in FC, IC, IF, IH(P), IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      A 1:50 dilution of a previous lot of this antibody immunoprecipitated PI3K, p85α using protein G:agarose beads.

      Flow Cytometry:
      A 1:50 dilution of a previous lot of this antibody stained cells for intracellular staining for flow cytometry analysis.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to the C-terminal region of human PI3 Kinase, p85α subunit.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the C-terminal domain of PI3 Kinase, p85α subunit.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteProven to react with human, mouse and rat.
      Reactivity with other species has not been confirmed.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryPhosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms.
      Gene Symbol
      • PIK3R1
      • GRB1
      • PI3K
      • p85-ALPHA
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P27986 # Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
      SIZE: 724 amino acids; 83598 Da
      SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR and/or BCR activation. Interacts with SOCS7. Interacts with RUFY3 (By similarity). Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. Interacts with CBLB. Interacts with HIV-1 Nef to activate the Nef associated p21-activated kinase (PAK). This interaction depends on the C- terminus of both proteins and leads to increased production of HIV. Interacts with HCV NS5A. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with IRS1.
      TISSUE SPECIFICITY: Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
      DOMAIN: SwissProt: P27986 The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
      PTM: Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.DISEASE:SwissProt: P27986 # Defects in PIK3R1 are a cause of severe insulin resistance.
      SIMILARITY: Belongs to the PI3K p85 subunit family. & Contains 1 Rho-GAP domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain.
      Molecular Weight85 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on A431 lysate.

      Western Blot Analysis:
      A 1:2,000 dilution of the lot detected endogenous PI3K, p85α in lysates from A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PI3 Kinase, p85 Rabbit Monoclonal - 2000377 2000377
      Anti-PI3 Kinase, p85 Rabbit Monoclonal - 2032697 2032697
      Anti-PI3 Kinase, p85 Rabbit Monoclonal - 2091716 2091716
      Anti-PI3 Kinase, p85 Rabbit Monoclonal - 2203062 2203062
      Anti-PI3 Kinase, p85 Rabbit Monoclonal - DAM1408852 DAM1408852
      Anti-PI3 Kinase, p85 Rabbit Monoclonal - DAM1414987 DAM1414987
      Anti-PI3 Kinase, p85 Rabbit Monoclonal - DAM1644590 DAM1644590
      Anti-PI3 Kinase, p85 Rabbit Monoclonal - DAM1662458 DAM1662458
      Anti-PI3 Kinase, p85 Rabbit Monoclonal - DAM1731527 DAM1731527
      Anti-PI3 Kinase, p85 Rabbit Monoclonal - DAM1767541 DAM1767541


      Reference overviewApplicationPub Med ID
      NSC-640358 acts as RXRα ligand to promote TNFα-mediated apoptosis of cancer cell.
      Chen, F; Chen, J; Lin, J; Cheltsov, AV; Xu, L; Chen, Y; Zeng, Z; Chen, L; Huang, M; Hu, M; Ye, X; Zhou, Y; Wang, G; Su, Y; Zhang, L; Zhou, F; Zhang, XK; Zhou, H
      Protein & cell  6  654-66  2015

      Show Abstract
      26156677 26156677
      Morphological evidences in circumvallate papilla and von Ebners' gland development in mice.
      Sohn, WJ; Gwon, GJ; An, CH; Moon, C; Bae, YC; Yamamoto, H; Lee, S; Kim, JY
      Anatomy & cell biology  44  274-83  2011

      Show Abstract
      22254156 22254156
      Positive modulation of AMPA receptors prevents downregulation of GluR2 expression and activates the Lyn-ERK1/2-CREB signaling in rat brain ischemia.
      Quan-Guang Zhang,Dong Han,Shu-Qun Hu,Chong Li,Chang-Zhou Yu,Ran Wang,Guang-Yi Zhang
      Hippocampus  20  2010

      Show Abstract
      19330848 19330848
      The interferon-gamma-induced murine guanylate-binding protein-2 inhibits rac activation during cell spreading on fibronectin and after platelet-derived growth factor treatment: role for phosphatidylinositol 3-kinase.
      Messmer-Blust, AF; Balasubramanian, S; Gorbacheva, VY; Jeyaratnam, JA; Vestal, DJ
      Molecular biology of the cell  21  2514-28  2010

      Show Abstract Full Text Article
      Immunoprecipitation20505078 20505078
      Recruitment of the tyrosine phosphatase Src homology 2 domain tyrosine phosphatase-2 to the p85 subunit of phosphatidylinositol-3 (PI-3) kinase is required for insulin-like growth factor-I-dependent PI-3 kinase activation in smooth muscle cells
      Kwon, Mijin, et al
      Endocrinology, 147:1458-65 (2006)  2006

      16306077 16306077
      Evidence that phosphatidylinositol 3-kinase- and mitogen-activated protein kinase kinase-4/c-Jun NH2-terminal kinase-dependent Pathways cooperate to maintain lung cancer cell survival
      Lee, Ho-Young, et al
      J Biol Chem, 278:23630-8 (2003)  2003

      12714585 12714585

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      Life Science Research > Antibodies and Assays > Primary Antibodies