05-1558 | Anti-PI3 Kinase b Antibody, p110b, clone 8C3.2

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, MWB, ICCMPurifiedMonoclonal Antibody
      Catalogue Number05-1558
      DescriptionAnti-PI3 Kinase b Antibody, p110b, clone 8C3.2
      Alternate Names
      • PI3-kinase p110 subunit beta
      • PtdIns-3-kinase p110
      • phosphatidylinositol 3-kinase, catalytic, beta polypeptide
      • phosphoinositide-3-kinase, catalytic, beta polypeptide
      • PI3K beta
      • PI3 K beta
      • PI3 kinase b
      Background InformationPI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
      Product Information
      • PC-12 cell lysate
      PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
      ApplicationThis Anti-PI3 Kinase b Antibody, p110b, clone 8C3.2 is validated for use in WB, IC for the detection of PI3 Kinase b.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution positively stained NIH3T3 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human PI3 Kinase b.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes PI3 Kinase b at the N-terminus.
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryPhosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM].
      Gene Symbol
      • PIK3CB
      • PI3Kbeta
      • PIK3C1
      Purification MethodIon Exchange
      UniProt Number
      UniProt SummaryFUNCTION: Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.

      CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate.

      PATHWAY: Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.

      SUBUNIT STRUCTURE: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit.

      TISSUE SPECIFICITY: Expressed ubiquitously.

      SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family.

      Contains 1 PI3K/PI4K domain.
      Molecular Weight~110 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in PC-12 cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected PI3 Kinase b on 10 µg of PC-12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PI3 Kinase b, p110b, clone 8C3.2 - 2377385 2377385
      Anti-PI3 Kinase b, p110b, clone 8C3.2 - 2441973 2441973
      Anti-PI3 Kinase b, p110b, clone 8C3.2 - 2246803 2246803
      Anti-PI3 Kinase b, p110b, clone 8C3.2 - 2562373 2562373
      Anti-PI3 Kinase b, p110b, clone 8C3.2 - 2999524 2999524
      Anti-PI3 Kinase b, p110b, clone 8C3.2 - NRG1849546 NRG1849546
      Anti-PI3 Kinase b, p110b, clone 8C3.2 -2496218 2496218
      Anti-PI3 Kinase b, p110b, clone 8C3.2 -2547218
      Anti-PI3 Kinase b, p110b, clone 8C3.2 -2547218 2547218
      Anti-PI3 Kinase b, p110b, clone 8C3.2 -2887644 2887644