07-675 | Anti-PIKE-L/S Antibody

07-675
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RIP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-675
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PIKE-L/S Antibody
      References
      Product Information
      FormatPurified
      Applications
      ApplicationDetect PIKE-L/S using this Anti-PIKE-L/S Antibody validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      Immunogen6His-tagged fusion protein corresponding to residues 268-384 of rat PIKE-L
      HostRabbit
      SpecificityPIKE-L and PIKE-S doublet
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CENTG1
      • GGAP2
      • PIKE
      • KIAA0167
      • FLJ16430
      • AGAP-2
      • AGAP2
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q99490 # GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system.
      SIZE: 1192 amino acids; 124674 Da
      SUBUNIT: Isoform 1 interacts with EPB41L1, PLCG1, NF2, HOMER1 and HOMER2 (By similarity). Isoform 2 interacts with activated AKT1 in the presence of guanine nucleotides, and with the AP-1 complex.
      SUBCELLULAR LOCATION: Isoform 1: Cytoplasm. Nucleus. & Isoform 2: Cytoplasm.TISSUE SPECIFICITY: Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart.
      DOMAIN: SwissProt: Q99490 G domain binds GTP and has GTPase activity. & Arf-GAP domain interacts with G domain and may regulate its GTPase activity. & Although both PH domains of isoforms 1 and 2 bind phospholipids, they differently regulate subcellular location. PH domain of isoform 1 directs the protein to the nucleus, but PH domain of isoform 2 directs it to the cytosol. PH domain of isoform 2 is required for binding to AP-1.
      SIMILARITY: Belongs to the centaurin gamma-like family. & Contains 2 ANK repeats. & Contains 1 Arf-GAP domain. & Contains 1 Miro domain. & Contains 1 PH domain.
      MISCELLANEOUS: Isoform 2 seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
      Molecular WeightPIKE-L, Mr 150kDa, and PIKE-S doublet Mr 98kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from PC12 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditionsstable for 2 years at -20°C from date of shipment
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PIKE-L/S (rabbit polyclonal IgG) -2483898 2483898
      Anti-PIKE-L/S - 30327 30327

      References

      Reference overviewPub Med ID
      PIKE GTPase: a novel mediator of phosphoinositide signaling.
      Ye, Keqiang and Snyder, Solomon H
      J. Cell. Sci., 117: 155-61 (2004)  2004

      Show Abstract
      14676271 14676271
      PIKE-A is amplified in human cancers and prevents apoptosis by up-regulating Akt.
      Ahn, Jee-Yin, et al.
      Proc. Natl. Acad. Sci. U.S.A., 101: 6993-8 (2004)  2004

      Show Abstract
      15118108 15118108
      Phospholipase C-gamma1: a phospholipase and guanine nucleotide exchange factor.
      Wang, Zhixiang and Moran, Michael F
      Mol. Interv., 2: 352-5,338 (2002)  2002

      Show Abstract
      14993410 14993410