07-264 | Anti-PKC ζ Antibody

07-264
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-264
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PKC ζ Antibody
      References
      Product Information
      FormatSerum
      Presentationantiserum containing 23% glycerol and 0.05% sodium azide
      Applications
      ApplicationDetect PKC ζ using this Anti-PKC ζ Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide corresponding to the C-terminal amino acids 577-592 (C-GFEYINPLLLSAEESV) of rat PKC-zeta
      HostRabbit
      SpecificityPKC zeta
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryProtein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
      Gene Symbol
      • PRKCZ
      • nPKC-zeta
      • PKC2
      • OTTHUMP00000044160
      Purification MethodAntiserum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q05513 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
      SIZE: 592 amino acids; 67660 Da
      SUBUNIT: Forms a ternary complex with SQSTM1 and KCNAB2. Forms another ternary complex with SQSTM1 and GABRR3. Forms a complex with SQSTM1 and MAP2K5 (By similarity). Interacts with PARD6A, PARD6B, PARD6G and SQSTM1. Part of a complex with PARD3, PARD6A or PARD6B or PARD6G and CDC42 or RAC1. Interacts with CENTA1. Forms a ternary complex composed of SQSTM1 and PAWR. Interacts directly with SQSTM1 (Probable). Interacts with IKBKB.
      SUBCELLULAR LOCATION: Cytoplasm. Endosome. Note=In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes.
      TISSUE SPECIFICITY: Expressed in brain, and to a lesser extent in lung, kidney and testis.
      DOMAIN: SwissProt: Q05513 The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B. & The C1 domain does not bind the diacylglycerol (DAG).
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 OPR domain. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain.
      Molecular Weight72kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from human A431 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PKC zeta - 2383530 2383530
      Anti-PKC zeta - 2025016 2025016
      Anti-PKC zeta - 2073121 2073121
      Anti-PKC zeta - 2225240 2225240
      Anti-PKC zeta - JBC1810822 JBC1810822
      Anti-PKC zeta - JBC1936136 JBC1936136
      Anti-PKC zeta - JBC1945031 JBC1945031
      Anti-PKC zeta -2639382 2639382
      Anti-PKC zeta Polyclonal Antibody 2978134
      Anti-PKC zeta; - 22080 22080

      References

      Reference overviewApplicationPub Med ID
      Phenotypical analysis of atypical PKCs in vivo function display a compensatory system at mouse embryonic day 7.5.
      Seidl, S; Braun, U; Roos, N; Li, S; Lüdtke, TH; Kispert, A; Leitges, M
      PloS one  8  e62756  2013

      Show Abstract
      23690951 23690951
      Laminin 511 partners with laminin 332 to mediate directional migration of Madin-Darby canine kidney epithelial cells.
      Greciano, PG; Moyano, JV; Buschmann, MM; Tang, J; Lu, Y; Rudnicki, J; Manninen, A; Matlin, KS
      Molecular biology of the cell  23  121-36  2012

      Show Abstract
      22031290 22031290
      Inhibition of PKMzeta in nucleus accumbens core abolishes long-term drug reward memory.
      Li, YQ; Xue, YX; He, YY; Li, FQ; Xue, LF; Xu, CM; Sacktor, TC; Shaham, Y; Lu, L
      The Journal of neuroscience : the official journal of the Society for Neuroscience  31  5436-46  2011

      Show Abstract
      Western Blotting21471379 21471379
      The interaction of mPar3 with the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity.
      Schwamborn, JC; Khazaei, MR; Püschel, AW
      The Journal of biological chemistry  282  35259-68  2007

      Show Abstract
      17906294 17906294
      DNA topoisomerase inhibitor, etoposide, enhances GC-box-dependent promoter activity via Sp1 phosphorylation.
      Ichiro Niina, Takeshi Uchiumi, Hiroto Izumi, Takayuki Torigoe, Tetsuro Wakasugi, Tomonori Igarashi, Naoya Miyamoto, Takamitsu Onitsuka, Masaki Shiota, Ryuichi Okayasu, Kazuo Chijiiwa, Kimitoshi Kohno
      Cancer science  98  858-63  2007

      Show Abstract
      17441964 17441964
      Connective tissue growth factor [CTGF]/CCN2 stimulates mesangial cell migration through integrated dissolution of focal adhesion complexes and activation of cell polarization.
      Crean, JK; Furlong, F; Finlay, D; Mitchell, D; Murphy, M; Conway, B; Brady, HR; Godson, C; Martin, F
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology  18  1541-3  2004

      Show Abstract
      15319369 15319369
      Targeted deletion of protein kinase C lambda reveals a distribution of functions between the two atypical protein kinase C isoforms.
      Rachel S Soloff, Carol Katayama, Meei Yun Lin, James R Feramisco, Stephen M Hedrick
      Journal of immunology (Baltimore, Md. : 1950)  173  3250-60  2004

      Show Abstract
      15322187 15322187
      Activation and translocation of PKCdelta is necessary for VEGF-induced ERK activation through KDR in HEK293T cells.
      Masamitsu Kuriyama, Taizo Taniguchi, Yasuhito Shirai, Atsuo Sasaki, Akihiko Yoshimura, Naoaki Saito
      Biochemical and biophysical research communications  325  843-51  2004

      Show Abstract
      15541367 15541367

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies