04-406 | Anti-PKCβII Antibody, rabbit monoclonal

04-406
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, IF, IP, WB, IH(P)RbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-406
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PKCβII Antibody, rabbit monoclonal
      Alternate Names
      • Protein Kinase C
      References
      Product Information
      FormatPurified
      Presentation100 µL of rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 0.09 M NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-PKCβII Antibody, rabbit is validated for use in FC, IF, IP, WB, IH(P) for the detection of PKCβII.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to a region in the C-terminus of human PKCβII.
      HostRabbit
      SpecificityRecognizes the C-terminus of PKCβII. It does not cross-react with the closely related PKCβI isoform.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
      Gene Symbol
      • PRKCB1
      • PRKCB
      • PRKCB2
      • PKCB
      • MGC41878
      • PKC-B
      • PKC-beta
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05771 # This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May be considered as a novel component of the NF-kappa-B signaling axis responsible for the survival and activation of B-cells after BCR cross-linking (By similarity).
      COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
      SIZE: 671 amino acids; 76869 Da
      SUBUNIT: Interacts with PDK1 (By similarity). Interacts in vitro with PRKCBP1.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity).
      PTM: Phosphorylation on Thr-500 of isoform beta-I, within the activation loop, renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Similarly, isoform beta-II is autophosphorylated on 'Thr-640' and 'Ser-659', subsequent to phosphorylation on Thr-500. Autophosphorylated on other sites i.e. in the N-terminal and hinge regions have no effect on PKC activity (By similarity).
      SIMILARITY: SwissProt: P05771 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
      Molecular Weight77 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PKC#946;II, rabbit monoclonal - 2036072 2036072
      Anti-PKC#946;II, rabbit monoclonal - NG1843036 NG1843036
      Anti-PKCII, rabbit monoclonal -
      Anti-PKCII, rabbit monoclonal - 2205904 2205904
      Anti-PKCII, rabbit monoclonal - DAM1778047 DAM1778047
      Anti-PKCbeta;II, rabbit monoclonal - 0611045815 0611045815
      Anti-PKCβII, rabbit monoclonal - 2377254 2377254
      Anti-PKCβII, rabbit monoclonal - 2399446 2399446
      Anti-PKCβII, rabbit monoclonal - 2440178 2440178
      Anti-PKCβII, rabbit monoclonal - 2452177 2452177

      References

      Reference overviewPub Med ID
      IL-33 attenuates anoxia/reoxygenation-induced cardiomyocyte apoptosis by inhibition of PKCβ/JNK pathway.
      Rui, T; Tang, Q
      PloS one  8  e56089  2013

      Show Abstract
      23457506 23457506
      Vitamin E isoforms differentially regulate intercellular adhesion molecule-1 activation of PKCα in human microvascular endothelial cells.
      Abdala-Valencia, H; Berdnikovs, S; Cook-Mills, JM
      PloS one  7  e41054  2012

      Show Abstract
      22815910 22815910
      Sodium butyrate-mediated differentiation of colorectal cancer cells: regulation of PKCbetaII by PI 3-kinase.
      Turecková, Jolana, et al.
      Int. J. Mol. Med., 15: 329-35 (2005)  2005

      Show Abstract
      15647851 15647851
      Isoenzyme-specific translocation of protein kinase C (PKC)betaII and not PKCbetaI to a juxtanuclear subset of recycling endosomes: involvement of phospholipase D
      Becker, Kevin P and Hannun, Yusuf A
      J Biol Chem, 279:28251-6 (2004)  2004

      15067001 15067001

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies