07-151 | Anti-PKR Antibody

07-151
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-151
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PKR Antibody
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationThis Anti-PKR Antibody is validated for use in IP, WB for the detection of PKR.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein corresponding to amino acids 1-90 of human PKR
      HostRabbit
      SpecificityPKR
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EIF2AK2
      • MGC126524
      • EIF2AK1
      • PRKR
      • PKR
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P19525 # Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
      SIZE: 551 amino acids; 62094 Da
      SUBUNIT: Homodimer. Interacts with STRBP (By similarity). Interacts with DNAJC3. Inhibited by direct interaction with viral proteins such as HCV E2, HCV NS5A and influenza A NS1. Activated by the interaction with HIV-1 Tat.
      PTM: Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
      SIMILARITY: SwissProt: P19525 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. & Contains 2 DRBM (double-stranded RNA-binding) domains. & Contains 1 protein kinase domain.
      Molecular Weight68kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PKR - 20222 20222
      Anti-PKR - DAM1791476 DAM1791476
      Anti-PKR -2556296 2556296

      References

      Reference overviewApplicationPub Med ID
      Species specificity of protein kinase r antagonism by cytomegalovirus TRS1 genes.
      Child, SJ; Brennan, G; Braggin, JE; Geballe, AP
      Journal of virology  86  3880-9  2012

      Show Abstract
      22278235 22278235
      A diminished activation capacity of the interferon-inducible protein kinase PKR in human T lymphocytes.
      Li, S, et al.
      Eur. J. Biochem., 267: 1598-606 (2000)  2000

      Show Abstract
      10712589 10712589
      The protein kinase PKR is required for p38 MAPK activation and the innate immune response to bacterial endotoxin.
      Goh, K C, et al.
      EMBO J., 19: 4292-7 (2000)  2000

      Show Abstract
      10944112 10944112
      The interferon-induced protein kinase (PKR), triggers apoptosis through FADD-mediated activation of caspase 8 in a manner independent of Fas and TNF-alpha receptors.
      Gil, J and Esteban, M
      Oncogene, 19: 3665-74 (2000)  2000

      Show Abstract
      10951573 10951573
      IRF-1 induced cell growth inhibition and interferon induction requires the activity of the protein kinase PKR.
      Kirchhoff, S, et al.
      Oncogene, 11: 439-45 (1995)  1995

      Show Abstract
      Immunoprecipitation7543195 7543195

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      07-886 Anti-phospho-PKR (Thr451) Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies