05-163 | Anti-PLCγ-1 Antibody

05-163
50 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Rb, BICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-163
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PLCγ-1 Antibody
      OverviewAn equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells1 and propagated as ascites. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.
      Alternate Names
      • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
      • Phosphoinositide phospholipase C
      • Phospholipase C-gamma-1
      • monophosphatidylinositol phosphodiesterase
      • phosphatidylinositol phospholipase C
      • phosphoinositidase C
      • phospholipase C gamma 1
      • phospholipase C, gamma 1
      • phospholipase C, gamma 1 (formerly subtype 148)
      • phospholipase C-148
      • triphosphoinositide phosphodiesterase
      Background InformationPhospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown.
      References
      Product Information
      FormatPurified
      Control
      • A431 cell lysate, HeLa cell lysate
        Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
      PresentationLiquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide.
      An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells (Suh, P.G., 1988) and propagated as ascites. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.
      Applications
      ApplicationAnti-PLCγ-1 Antibody detects level of PLCγ-1 & has been published & validated for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 µg of a previous lot of antibody immunoprecipitated PLCγ-1 from non-stimulated A431 cell lysate, previous lots immunoprecipitated PLCγ-1 from rabbit brain cytosol preparation.

      Immunocytochemistry:
      5-10 µg/mL of previous lots of this antibody detected PLCγ-1 on ethanol-acetic acid [95:5] fixed A431 cells.
      Biological Information
      ImmunogenFive individual clones raised against purified soluble phospholipase C (PLC)γ-1 from bovine brain.
      CloneClonal blend B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.
      HostMouse
      SpecificityThis antibody recognizes PLCγ-1, Mr 135 kDa. Reacts with some non-PLCγ-1 SH-3 containing proteins, including nck, Mr 47kDa (Park, D., 1992).
      Isotypemixed IgGs
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Bovine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • PLCG1
      • PLC1
      • PLCgamma1
      • PLC148
      • PLC-II
      • PLC-148
      • 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
      • PLC-gamma-1
      • inositoltrisphosphohydrolase
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P19174 # PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
      COFACTOR: Calcium.
      SIZE: 1290 amino acids; 148532 Da
      SUBUNIT: Interacts with CENTG1 via its SH3 domain (By similarity). Interacts with phosphorylated LAT upon TCR activation. Interacts with the Pro-rich domain of TNK1 via its SH3 domain. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor- dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts via its SH3 domain with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1 (By similarity).
      PTM: The receptor-mediated activation of PLC-gamma-1 and PLC- gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. & Ubiquitinated by CBLB in activated T-cells (By similarity).
      SIMILARITY: SwissProt: P19174 ## Contains 1 C2 domain. & Contains 1 EF-hand domain. & Contains 2 PH domains. & Contains 1 PI-PLC X-box domain. & Contains 1 PI-PLC Y-box domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain. ...
      Molecular Weight135 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in human A431 carcinoma cells, rabbit brain cytosol, and bovine brain cytosol preparations.

      Western Blot Analysis:
      0.5-2 µg/mL of this lot detected PLCγ-1 in human A431 carcinoma cells; previous lots detected PKCγ-1 in rabbit brain cytosol and bovine brain cytosol preparations.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of shipment.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PLC-1 - 2200856 2200856
      Anti-PLC-1 - 2280872 2280872
      Anti-PLC-1 - DAM1411320 DAM1411320
      Anti-PLC-1 - DAM1692583 DAM1692583
      Anti-PLC-1 - DAM1731455 DAM1731455
      Anti-PLC-1 - DAM1754420 DAM1754420
      Anti-PLC-1 - JBC1831378 JBC1831378
      Anti-PLCgamma;-1 - 15655 15655
      Anti-PLCgamma;-1 - 17594 17594
      Anti-PLCgamma;-1 - 18901 18901

      References

      Reference overviewApplicationPub Med ID
      Inhibition of the kinase Csk in thymocytes reveals a requirement for actin remodeling in the initiation of full TCR signaling.
      Tan, YX; Manz, BN; Freedman, TS; Zhang, C; Shokat, KM; Weiss, A
      Nature immunology  15  186-94  2014

      Show Abstract
      24317039 24317039
      Vitamin D-binding protein controls T cell responses to vitamin D.
      Kongsbak, M; von Essen, MR; Levring, TB; Schjerling, P; Woetmann, A; Ødum, N; Bonefeld, CM; Geisler, C
      BMC immunology  15  35  2014

      Show Abstract
      Western Blotting25230725 25230725
      Glucocorticoid receptors recruit the CaMKIIα-BDNF-CREB pathways to mediate memory consolidation.
      Chen, DY; Bambah-Mukku, D; Pollonini, G; Alberini, CM
      Nature neuroscience  15  1707-14  2012

      Show Abstract
      23160045 23160045
      Lysosomal accumulation of Trk protein in brain of GM₁ - gangliosidosis mouse and its restoration by chemical chaperone.
      Takamura A, Higaki K, Ninomiya H, Takai T, Matsuda J, Iida M, Ohno K, Suzuki Y, Nanba E
      Journal of neurochemistry  118  399-406. doi  2011

      Show Abstract
      21574998 21574998
      Vitamin D controls T cell antigen receptor signaling and activation of human T cells.
      von Essen MR, Kongsbak M, Schjerling P, Olgaard K, Odum N, Geisler C
      Nat Immunol  11  344-9. Epub 2010 Mar 7.  2010

      Show Abstract
      20208539 20208539
      Host range and genetic relatedness of Colletotrichum acutatum isolates from fruit crops and leatherleaf fern in Florida.
      S J MacKenzie,N A Peres,M P Barquero,L F Arauz,L W Timmer
      Phytopathology  99  2009

      Show Abstract
      19351258 19351258
      Tyrosine kinase regulation of nuclear envelope assembly.
      Richard D Byrne, Banafshé Larijani, Dominic L Poccia, Richard D Byrne, Banafshé Larijani, Dominic L Poccia, Richard D Byrne, Banafshé Larijani, Dominic L Poccia
      Advances in enzyme regulation  49  148-56  2009

      19344633 19344633
      Atlastin GTPases are required for Golgi apparatus and ER morphogenesis.
      Rismanchi, N; Soderblom, C; Stadler, J; Zhu, PP; Blackstone, C
      Human molecular genetics  17  1591-604  2008

      Show Abstract Full Text Article
      18270207 18270207
      Persistence of cooperatively stabilized signaling clusters drives T-cell activation.
      Bunnell, SC; Singer, AL; Hong, DI; Jacque, BH; Jordan, MS; Seminario, MC; Barr, VA; Koretzky, GA; Samelson, LE
      Molecular and cellular biology  26  7155-66  2006

      Show Abstract Full Text Article
      16980618 16980618
      Role of the pleckstrin homology domain of PLCgamma1 in its interaction with the insulin receptor.
      Kwon, YK; Jang, HJ; Kole, S; He, HJ; Bernier, M
      The Journal of cell biology  163  375-84  2003

      Show Abstract Full Text Article
      14568990 14568990

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      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies