05-608 | Anti-PLD Antibody, clone SKB2

05-608
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-608
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PLD Antibody, clone SKB2
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, and 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect PLD using this Anti-PLD Antibody, clone SKB2 validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST fusion-protein corresponding to residues 326-504 of human PLD 1
      Cloneclone SKB2
      HostMouse
      SpecificityPLD 1 and PLD 2
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryPhosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis (Hammond et al., 1995 [PubMed 8530346]).[supplied by OMIM]
      Gene Symbol
      • PLD1
      • hPLD1
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13393 # Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).
      SIZE: 1074 amino acids; 124184 Da
      SUBUNIT: Interacts with PIP5K1A.
      SUBCELLULAR LOCATION: Cytoplasm, perinuclear region (By similarity). Endoplasmic reticulum (By similarity). Golgi apparatus (By similarity). Late endosome (By similarity).
      TISSUE SPECIFICITY: Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
      SIMILARITY: SwissProt: Q13393 ## Belongs to the phospholipase D family. & Contains 1 PH domain. & Contains 2 PLD phosphodiesterase domains. & Contains 1 PX (phox homology) domain.
      Molecular Weight116 kDa (PLD1), 100 kDa (PLD2)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PLD, clone SKB2 (mouse monoclonal IgG) - 2424746 2424746
      Anti-PLD, clone SKB2 (mouse monoclonal IgG) - 2428656 2428656
      Anti-PLD, clone SKB2 (mouse monoclonal IgG) - 2197498 2197498
      Anti-PLD, clone SKB2 (mouse monoclonal IgG) - NG1941675 NG1941675
      Anti-PLD, clone SKB2 - 20375 20375
      Anti-PLD, clone SKB2 - 2040086 2040086
      Anti-PLD, clone SKB2 - 25277 25277
      Anti-PLD, clone SKB2 - DAM1588208 DAM1588208
      Anti-PLD, clone SKB2 - JBC1900500 JBC1900500

      References

      Reference overviewApplicationPub Med ID
      Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells.
      Cai, K; Lucki, NC; Sewer, MB
      Biochimica et biophysica acta  1841  552-62  2014

      Show Abstract
      Western Blotting24369117 24369117
      Phospholipase D1 regulates secretagogue-stimulated insulin release in pancreatic beta-cells.
      Hughes, WE; Elgundi, Z; Huang, P; Frohman, MA; Biden, TJ
      The Journal of biological chemistry  279  27534-41  2004

      Show Abstract
      15087463 15087463