05-421 | Anti-PP2A Antibody, C subunit, clone 1D6

05-421
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, B, Rb, Xn, Yeast (S. cerevisiae)ICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-421
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PP2A Antibody, C subunit, clone 1D6
      Alternate Names
      • Replication protein C
      • protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
      • protein phosphatase 2, catalytic subunit, alpha isoform
      • serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
      Background InformationType 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal downmodulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.
      References
      Product Information
      FormatPurified
      Control
      • HeLa whole cell lysate, mouse NIH3T3 cells, A431 cell lysate.
        Included Positive Antigen Control: Catalog # 12-301, non stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1M Tris-Glycine, pH 7.4, 0.15 M, NaCl, and 0.05% sodium azide. Frozen solution.
      Applications
      ApplicationDetect PP2A using this Anti-PP2A Antibody, C subunit, clone 1D6 validated for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 µg of a previous lot immunoprecipitated PP2A from 500 µg of A431 RIPA lysate. Note: This antibody has been reported not to block phosphatase activity.

      Immunocytochemistry:
      5-10 µg/mL of a previous lot of this antibody showed positive immunostaining for PP2A in A431 cells fixed for 10 minutes with 4% paraformaldehyde in PBS.
      Biological Information
      ImmunogenA 16 residue synthetic peptide (C-RGEPHVTRRTPDYFL) corresponding to amino acids 295-309 of the 36 kDa catalytic subunit of human protein phosphatase 2A (PP2A). Clone 1D6.
      Epitopea.a. 295-309
      Clone1D6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes PP2A catalytic subunit, Mr 36 kDa.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Bovine
      • Rabbit
      • Xenopus
      • Yeast (S. cerevisiae)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
      Gene Symbol
      • PPP2CA
      • PP2CA
      • PP2A-alpha
      • PP2Ac
      • RP-C
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P67775 # PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
      COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).
      SIZE: 309 amino acids; 35594 Da
      SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
      SUBCELLULAR LOCATION: Cytoplasm.
      PTM: Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. & Phosphorylation of either threonine (by autophosphorylation- activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
      SIMILARITY: SwissProt: P67775 ## Belongs to the PPP phosphatase family. PP-1 subfamily.
      Molecular Weight36 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on human A431 or murine 3T3/NIH RIPA cell lysates.

      Western Blot Analysis:
      0.5-2 µg/mL of this lot detected PP2A in human A431 and previously in murine 3T3 RIPA cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PP2A, C subunit, clone 1D6 - 2137086 2137086
      Anti-PP2A, C subunit, clone 1D6 - 2459607 2459607
      Anti-PP2A, C subunit, clone 1D6 - 0608036855 0608036855
      Anti-PP2A, C subunit, clone 1D6 - 0701050857 0701050857
      Anti-PP2A, C subunit, clone 1D6 - 16199 16199
      Anti-PP2A, C subunit, clone 1D6 - 18593 18593
      Anti-PP2A, C subunit, clone 1D6 - 1970366 1970366
      Anti-PP2A, C subunit, clone 1D6 - 2015409 2015409
      Anti-PP2A, C subunit, clone 1D6 - 20961 20961
      Anti-PP2A, C subunit, clone 1D6 - 2144398 2144398

      References

      Reference overviewApplicationSpeciesPub Med ID
      MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C.
      Voets, E; Wolthuis, R
      Biology open  4  484-95  2015

      Show Abstract
      25750436 25750436
      Regulation of autophagy by coordinated action of mTORC1 and protein phosphatase 2A.
      Wong, PM; Feng, Y; Wang, J; Shi, R; Jiang, X
      Nature communications  6  8048  2015

      Show Abstract
      26310906 26310906
      Mutant TDP-43 deregulates AMPK activation by PP2A in ALS models.
      Perera, ND; Sheean, RK; Scott, JW; Kemp, BE; Horne, MK; Turner, BJ
      PloS one  9  e90449  2014

      Show Abstract
      Western Blotting24595038 24595038
      Cell- and virus-mediated regulation of the barrier-to-autointegration factor's phosphorylation state controls its DNA binding, dimerization, subcellular localization, and antipoxviral activity.
      Jamin, A; Wicklund, A; Wiebe, MS
      Journal of virology  88  5342-55  2014

      Show Abstract
      24600006 24600006
      Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers.
      Williams, BC; Filter, JJ; Blake-Hodek, KA; Wadzinski, BE; Fuda, NJ; Shalloway, D; Goldberg, ML
      eLife  3  e01695  2014

      Show Abstract
      24618897 24618897
      Cytoplasmic SET induces tau hyperphosphorylation through a decrease of methylated phosphatase 2A.
      Chasseigneaux, S; Clamagirand, C; Huguet, L; Gorisse-Hussonnois, L; Rose, C; Allinquant, B
      BMC neuroscience  15  82  2014

      Show Abstract
      Western Blotting24981783 24981783
      Evidence toward a dual phosphatase mechanism that restricts Aurora A (Thr-295) phosphorylation during the early embryonic cell cycle.
      Kang, Q; Srividhya, J; Ipe, J; Pomerening, JR
      The Journal of biological chemistry  289  17480-96  2014

      Show Abstract
      24825897 24825897
      Altered protein phosphatase 2A methylation and Tau phosphorylation in the young and aged brain of methylenetetrahydrofolate reductase (MTHFR) deficient mice.
      Sontag, JM; Wasek, B; Taleski, G; Smith, J; Arning, E; Sontag, E; Bottiglieri, T
      Frontiers in aging neuroscience  6  214  2014

      Show Abstract
      Western Blotting25202269 25202269
      F-box protein FBXL16 binds PP2A-B55α and regulates differentiation of embryonic stem cells along the FLK1+ lineage.
      Honarpour, N; Rose, CM; Brumbaugh, J; Anderson, J; Graham, RL; Sweredoski, MJ; Hess, S; Coon, JJ; Deshaies, RJ
      Molecular & cellular proteomics : MCP  13  780-91  2014

      Show Abstract
      Western Blotting24390425 24390425
      Non-motor parkinsonian pathology in aging A53T α-synuclein mice is associated with progressive synucleinopathy and altered enzymatic function.
      Farrell, KF; Krishnamachari, S; Villanueva, E; Lou, H; Alerte, TN; Peet, E; Drolet, RE; Perez, RG
      Journal of neurochemistry  128  536-46  2014

      Show Abstract
      Immunohistochemistry24117685 24117685

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      Included Positive Control

      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies