07-708 | Anti-Pals1 Antibody

07-708
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, RIP, WB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-708
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Pals1 Antibody
      References
      Product Information
      FormatAffinity Purified
      Applications
      ApplicationDetect Pals1 using this Anti-Pals1 Antibody validated for use in IP, WB & IC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (C-YNANKNDDYDNE) corresponding to amino acids 450-461 of human Pals1 (palmitoylated 5 protein), also known as MAGUK p55 subfamily member 5 and stardust (Drosophila). The immunizing sequence is identical in orangutan, chimpanzee, chicken, mouse, rat, canine and fish.
      HostRabbit
      SpecificityRecognizes Pals1
      IsotypeIgG
      Species Reactivity
      • Canine
      • Rat
      Species Reactivity NotePredicted to cross-react with human, orangutan, chimpanzee, mouse, chicken and fish based on sequence homology
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMembers of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like (see DLG1; MIM 601014), ZO1-like (see TJP1; MIM 601009), p55-like (see MPP1; MIM 305360), and LIN2-like (see CASK; MIM 300172), based on their size and the presence of additional domains (Tseng et al., 2001 [PubMed 11311936]). MPP5 is a member of the p55-like MAGUK subfamily.[supplied by OMIM]
      Gene Symbol
      • MPP5
      • FLJ12615
      • PALS1
      • stardust
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q8N3R9 # May play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of VIL2 to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton (By similarity).
      SIZE: 675 amino acids; 77294 Da
      SUBUNIT: Interacts with the LIN7 proteins, CRB3 and MPDZ. Interacts with INADL, PARD6B, SC6A1 and VIL2 (By similarity). Forms a complex with CRB1 and MPP4. Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3.
      SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. Intracytoplasmic membrane; Peripheral membrane protein. Note=Localized to the tight junctions of epithelial cells and a subset of intracellular vesicles. In the retina, detected at the outer limiting membranes (OLM), apical to the adherens junction (AJ), where it colocalizes with CRB1. Localized to the Purkinje cell body and axon.
      TISSUE SPECIFICITY: Expressed in the retina (at protein level).DOMAIN:SwissProt: Q8N3R9 The L27 domain 1 functions in targeting to the tight junctions by binding to INADL (By similarity). & The PDZ domain binds to the C-terminus of SC6A1 (By similarity).
      SIMILARITY: Belongs to the MAGUK family. & Contains 1 guanylate kinase-like domain. & Contains 2 L27 domains. & Contains 1 PDZ (DHR) domain. & Contains 1 SH3 domain.
      Molecular WeightMr 77kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C from date of shipment
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Pals1 (rabbit immunoaffinity purified IgG) - 2239847 2239847
      Anti-Pals1 (rabbit immunoaffinity purified IgG) 2890847
      Anti-Pals1 (rabbit immunoaffinity purified IgG) 3006772
      Anti-Pals1 - 30445 30445
      Anti-Pals1 -2649135 2649135
      Anti-Pals1 -2694483 2694483
      Anti-Pals1 -2765943 2765943
      Anti-Pals1 -2818716 2818716

      References

      Reference overviewPub Med ID
      Neurite Mistargeting and Inverse Order of Intraretinal Vascular Plexus Formation Precede Subretinal Vascularization in Vldlr Mutant Mice.
      Johnson, V; Xiang, M; Chen, Z; Junge, HJ
      PloS one  10  e0132013  2015

      Show Abstract
      26177550 26177550
      Dual function of Yap in the regulation of lens progenitor cells and cellular polarity.
      Song, JY; Park, R; Kim, JY; Hughes, L; Lu, L; Kim, S; Johnson, RL; Cho, SH
      Developmental biology  386  281-90  2014

      Show Abstract
      24384391 24384391
      Participation of the cell polarity protein PALS1 to T-cell receptor-mediated NF-κB activation.
      Carvalho, Gabrielle, et al.
      PLoS ONE, 6: e18159 (2011)  2011

      Show Abstract
      21479189 21479189
      Cdc42- and IRSp53-dependent contractile filopodia tether presumptive lens and retina to coordinate epithelial invagination.
      Chauhan, BK; Disanza, A; Choi, SY; Faber, SC; Lou, M; Beggs, HE; Scita, G; Zheng, Y; Lang, RA
      Development (Cambridge, England)  136  3657-67  2009

      Show Abstract
      19820184 19820184
      MPP5 recruits MPP4 to the CRB1 complex in photoreceptors.
      Kantardzhieva, Albena, et al.
      Invest. Ophthalmol. Vis. Sci., 46: 2192-201 (2005)  2005

      Show Abstract
      15914641 15914641
      PALS1 specifies the localization of ezrin to the apical membrane of gastric parietal cells.
      Cao, Xinwang, et al.
      J. Biol. Chem., 280: 13584-92 (2005)  2005

      Show Abstract
      15677456 15677456
      Loss of PALS1 expression leads to tight junction and polarity defects.
      Straight, Samuel W, et al.
      Mol. Biol. Cell, 15: 1981-90 (2004)  2004

      Show Abstract
      14718565 14718565
      Structural basis for L27 domain-mediated assembly of signaling and cell polarity complexes.
      Li, Yuanhe, et al.
      EMBO J., 23: 2723-33 (2004)  2004

      Show Abstract
      15241471 15241471
      Direct interaction of two polarity complexes implicated in epithelial tight junction assembly.
      Hurd, Toby W, et al.
      Nat. Cell Biol., 5: 137-42 (2003)  2003

      12545177 12545177
      The Maguk protein, Pals1, functions as an adapter, linking mammalian homologues of Crumbs and Discs Lost.
      Roh, Michael H, et al.
      J. Cell Biol., 157: 161-72 (2002)  2002

      Show Abstract
      11927608 11927608