05-205 | Anti-Phospholamban Antibody, clone A1

05-205
50 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, M, Po, R, BELISA, ICC, IHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-205
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phospholamban Antibody, clone A1
      Alternate Names
      • cardiac phospholamban
      Background InformationPhospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.
      References
      Product Information
      FormatPurified
      Control
      • Bovine cardiac preparation.
      PresentationPurified mouse monoclonal IgG1 in buffer containing 20 mM Tris-HCI, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.
      Applications
      ApplicationDetect Phospholamban using this Anti-Phospholamban Antibody, clone A1 validated for use in ELISA, IC, IH, IP & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 μg of a previous lot immunoprecipitated phospholamban from 500 μg of bovine cardiac preparation

      Immunohistochemistry:
      10 μg/mL of previous lots of this antibody detected phospholamban in frozen rat heart tissue sections, fixed for either 5 minutes with ice cold ethanol:acetic acid [95:5] or for 30 minutes with 4% paraformaldehyde, 4% sucrose in PBS, followed by permeabilization with 0.25% Triton X-100 in PBS. Also reported by an independent laboratory (Slack, J. P., 1997).

      Immunocytochemisty:
      Recommended.
      Biological Information
      ImmunogenBovine phospholamban purified from bovine cardiac sarcoplasmic reticulum. Clone A1.
      CloneA1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes and is specific for the four forms of phospholamban: phosphorylated and non-phosphorylated phospholamban and the breakdown protein derivatives from the sarcoplasmic reticulum.
      IsotypeIgG1
      Species Reactivity
      • Canine
      • Mouse
      • Pig
      • Rat
      • Bovine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.
      Gene Symbol
      • PLN
      • CMD1P
      • PLB
      • phospholamban
      Modifications
      • Phosphorylation
      Purification MethodHydroxylapatite chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P26678 # Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum.
      SIZE: 52 amino acids; 6109 Da
      SUBUNIT: Homopentamer.
      SUBCELLULAR LOCATION: Membrane.
      TISSUE SPECIFICITY: Heart.
      PTM: Phosphorylated in response to beta-adrenergic stimulation.
      DISEASE:SwissProt: P26678 # Defects in PLN are the cause of dilated cardiomyopathy 1P (CMD1P) [MIM:609909]. Cardiomyopathy is a disorder characterized by cardiac dilation and reduced systolic function.
      SIMILARITY: SwissProt: P26678 ## Belongs to the phospholamban family.
      Molecular Weight25 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated on bovine cardiac preparation (do not boil cardiac preparations).

      Western Blot Analysis: 0.5-2 μg/mL of this lot detected phospholamban in 20 μg bovine cardiac preparation. DO NOT BOIL CARDIAC PREPARATION. (Most, P., 2003)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Phospholamban, clone A1 2474884
      Anti-Phospholamban, clone A1 - 2137996 2137996
      Anti-Phospholamban, clone A1 - 2387806 2387806
      Anti-Phospholamban, clone A1 - 2436413 2436413
      Anti-Phospholamban, clone A1 - 0606032063 0606032063
      Anti-Phospholamban, clone A1 - 14658 14658
      Anti-Phospholamban, clone A1 - 16717 16717
      Anti-Phospholamban, clone A1 - 17778 17778
      Anti-Phospholamban, clone A1 - 1988739 1988739
      Anti-Phospholamban, clone A1 - 21871 21871

      References

      Reference overviewApplicationPub Med ID
      Long term ablation of protein kinase A (PKA)-mediated cardiac troponin I phosphorylation leads to excitation-contraction uncoupling and diastolic dysfunction in a knock-in mouse model of hypertrophic cardiomyopathy.
      Dweck, D; Sanchez-Gonzalez, MA; Chang, AN; Dulce, RA; Badger, CD; Koutnik, AP; Ruiz, EL; Griffin, B; Liang, J; Kabbaj, M; Fincham, FD; Hare, JM; Overton, JM; Pinto, JR
      The Journal of biological chemistry  289  23097-111  2014

      Show Abstract
      Western Blotting24973218 24973218
      Inhibition of miR-25 improves cardiac contractility in the failing heart.
      Wahlquist, C; Jeong, D; Rojas-Muñoz, A; Kho, C; Lee, A; Mitsuyama, S; van Mil, A; Park, WJ; Sluijter, JP; Doevendans, PA; Hajjar, RJ; Mercola, M
      Nature  508  531-5  2014

      Show Abstract
      24670661 24670661
      Pak1 is required to maintain ventricular Ca²⁺ homeostasis and electrophysiological stability through SERCA2a regulation in mice.
      Wang, Y; Tsui, H; Ke, Y; Shi, Y; Li, Y; Davies, L; Cartwright, EJ; Venetucci, L; Zhang, H; Terrar, DA; Huang, CL; Solaro, RJ; Wang, X; Lei, M
      Circulation. Arrhythmia and electrophysiology  7  938-48  2014

      Show Abstract
      25217043 25217043
      Anthrax lethal toxin induces acute diastolic dysfunction in rats through disruption of the phospholamban signaling network.
      Golden, HB; Watson, LE; Nizamutdinov, D; Feng, H; Gerilechaogetu, F; Lal, H; Verma, SK; Mukhopadhyay, S; Foster, DM; Dillmann, WH; Dostal, DE
      International journal of cardiology  168  3884-95  2013

      Show Abstract
      23907041 23907041
      The effects of neuregulin on cardiac Myosin light chain kinase gene-ablated hearts.
      Chang, AN; Huang, J; Battiprolu, PK; Hill, JA; Kamm, KE; Stull, JT
      PloS one  8  e66720  2013

      Show Abstract
      23776695 23776695
      Impaired contractile function due to decreased cardiac myosin binding protein C content in the sarcomere.
      Cheng, Y; Wan, X; McElfresh, TA; Chen, X; Gresham, KS; Rosenbaum, DS; Chandler, MP; Stelzer, JE
      American journal of physiology. Heart and circulatory physiology  305  H52-65  2013

      Show Abstract
      23666674 23666674
      Fructose diet treatment in mice induces fundamental disturbance of cardiomyocyte Ca2+ handling and myofilament responsiveness.
      Mellor, KM; Wendt, IR; Ritchie, RH; Delbridge, LM
      American journal of physiology. Heart and circulatory physiology  302  H964-72  2012

      Show Abstract
      Western Blotting22198170 22198170
      Cardiac remodeling and myocardial dysfunction in obese spontaneously hypertensive rats.
      Linz, D; Hohl, M; Mahfoud, F; Reil, JC; Linz, W; Hübschle, T; Juretschke, HP; Neumann-Häflin, C; Rütten, H; Böhm, M
      Journal of translational medicine  10  187  2012

      Show Abstract
      Western Blotting22963383 22963383
      Changes in myofilament proteins, but not Ca²⁺ regulation, are associated with a high-fat diet-induced improvement in contractile function in heart failure.
      Cheng, Y; Li, W; McElfresh, TA; Chen, X; Berthiaume, JM; Castel, L; Yu, X; Van Wagoner, DR; Chandler, MP
      American journal of physiology. Heart and circulatory physiology  301  H1438-46  2011

      Show Abstract
      21765056 21765056
      Endotoxin-induced uveitis is primarily dependent on radiation-resistant cells and on MyD88 but not TRIF.
      Kezic J, Taylor S, Gupta S, Planck SR, Rosenzweig HL, Rosenbaum JT
      Journal of leukocyte biology  90  305-11. Epub 2011 May 24.  2011

      Show Abstract
      21610198 21610198

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies