05-1000MG | Anti-Phosphoserine Antibody, clone 4A4

1 mg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtWB, IF, FC, IH(P), ELISAMPurifiedMonoclonal Antibody
      Catalogue Number05-1000MG
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphoserine Antibody, clone 4A4
      Background InformationThe identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (greater than 95%).
      Product Information
      • Suggested Positive Control: Cat.# 12-628, Calyculin A/Okadaic-treated A431 cell lysate is recommended as a positive control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at –20ºC. The lysate is stable for 6 months at –20ºC.
      Presentation1 mL of PBS
      ApplicationThis Anti-Phosphoserine Antibody, clone 4A4 is validated for use in WB, IF, FC, IH(P), ELISA for the detection of Phosphoserine.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      • ELISA
      Biological Information
      ImmunogenPhosphoserine coupled to KLH
      SpecificitySerine-phosphorylated proteins from all species
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      • Phosphorylation
      Purification MethodProtein G-Sepharose™ Chromatography
      Molecular WeightDependent upon the molecular weight of the serine phosphorylated protein being detected.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment.
      For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      05-1000 Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) Show Pricing & Availability
      05-1000X Anti-Phosphoserine Antibody, clone 4A4 Show Pricing & Availability

      Product Families


      Life Science Research > Antibodies and Assays > Primary Antibodies