06-427 | Anti-Phosphotyrosine Antibody

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPRbAffinity PurifiedPolyclonal Antibody
      Catalogue Number06-427
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphotyrosine Antibody
      Background InformationThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Phosphotyrosine Antibody detects level of Phosphotyrosine & has been published & validated for use in IP & WB.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected Phosphotyrosine in 10 µg of EGF treated A431 with Lambda Phosphatase tissue lysate.
      Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated Phosphotyrosine in 0.5 mg of EGF treated A431 cell lysate
      Biological Information
      ImmunogenIn order to produce broad spectrum polyclonal phosphotyrosine antibodies, rabbits were immunized with three phosphorylated immunogens: (1) phosphotyrosine covalently linked to KLH; (2) the c-Src carboxyl terminal regulatory phosphopeptide (TS-T-E-P-Q-pY-Q-P-G-E-N-L; Catalog # 12-218) covalently linked to KLH, and; (3) a phosphopeptide associated with high tyrosine kinase activity in human lymphocytes (R-R-L-I-E-D-A-E-pY-A-A-R-G; Catalog # 12-217) covalently linked to KLH. Both of the phosphopeptide haptens serve as strong substrates for tyrosine phosphatases and are part of the two colorimetric tyrosine phosphatase kits provided by Upstate, Inc. (Catalog # 17-125, 17-126).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThe immunoreactivity of the antibody is totally inhibited by the use of 100 mM phenyl phosphate, a phosphotyrosine analog. The phosphotyrosine antibody is purified by immunoaffinity chromatography using either a dual phospho-peptide gel or a BSA-phosphotyrosine gel. All of the phosphotyrosine immunoreactivity present in the antisera is immunoadsorbed whether the antibody is purified by either gel indicating that the antibody is not sequence-specific but specific for phosphotyrosine residues.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with All based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      • Phosphorylation
      Purification MethodAffinity Purfied
      Molecular WeightVaries
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in EGF treated A431 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Phosphotyrosine in 10 µg of EGF treated A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Phosphotyrosine - 15629 15629
      Anti-Phosphotyrosine - 15651 15651
      Anti-Phosphotyrosine - 16206 16206
      Anti-Phosphotyrosine - 17798 17798
      Anti-Phosphotyrosine - 19078 19078
      Anti-Phosphotyrosine - 22726 22726
      Anti-Phosphotyrosine - 25163 25163
      Anti-Phosphotyrosine - 27337 27337
      Anti-Phosphotyrosine - 28136 28136
      Anti-Phosphotyrosine - 31999 31999


      Reference overviewApplicationPub Med ID
      Role of plasma membrane caveolae/lipid rafts in VEGF-induced redox signaling in human leukemia cells.
      Caliceti, C; Zambonin, L; Rizzo, B; Fiorentini, D; Vieceli Dalla Sega, F; Hrelia, S; Prata, C
      BioMed research international  2014  857504  2014

      Show Abstract
      24738074 24738074
      The role of PTEN in chronic growth hormone-induced hepatic insulin resistance.
      Gao, Y; Su, P; Wang, C; Zhu, K; Chen, X; Liu, S; He, J
      PloS one  8  e68105  2013

      Show Abstract
      23840818 23840818
      Effect of plasma membrane cholesterol depletion on glucose transport regulation in leukemia cells.
      Caliceti, C; Zambonin, L; Prata, C; Vieceli Dalla Sega, F; Hakim, G; Hrelia, S; Fiorentini, D
      PloS one  7  e41246  2012

      Show Abstract
      22859971 22859971
      IGF-1 Receptors in Xenopus laevis Ovarian Follicle Cells Support the Oocyte Maturation Response.
      Sadler SE, Angleson JK, Dsouza M
      Biology of reproduction  82  591-8  2010

      Show Abstract
      19923252 19923252
      Physical and functional interaction between polyoma virus middle T antigen and insulin and IGF-I receptors is required for oncogene activation and tumour initiation.
      Novosyadlyy, R; Vijayakumar, A; Lann, D; Fierz, Y; Kurshan, N; LeRoith, D
      Oncogene  28  3477-86  2009

      Show Abstract Full Text Article
      19617901 19617901
      Chronic oxidative stress causes amplification and overexpression of ptprz1 protein tyrosine phosphatase to activate beta-catenin pathway.
      Yu-Ting Liu, Donghao Shang, Shinya Akatsuka, Hiroki Ohara, Khokon Kumar Dutta, Katsura Mizushima, Yuji Naito, Toshikazu Yoshikawa, Masashi Izumiya, Kouichiro Abe, Hitoshi Nakagama, Noriko Noguchi, Shinya Toyokuni
      The American journal of pathology  171  1978-88  2007

      Show Abstract Full Text Article
      18055543 18055543
      Inhibition of epidermal growth factor receptor signalling reduces hypercalcaemia induced by human lung squamous-cell carcinoma in athymic mice.
      Lorch, G; Gilmore, JL; Koltz, PF; Gonterman, RM; Laughner, R; Lewis, DA; Konger, RL; Nadella, KS; Toribio, RE; Rosol, TJ; Foley, J
      British journal of cancer  97  183-93  2007

      Show Abstract
      17533397 17533397
      Wnt5a is required for proper mammary gland development and TGF-beta-mediated inhibition of ductal growth.
      Roarty, K; Serra, R
      Development (Cambridge, England)  134  3929-39  2007

      Show Abstract
      Western Blotting17898001 17898001
      Phosphoinositide 3-kinase, Src, and Akt modulate acute ventilation-induced vascular permeability increases in mouse lungs.
      Miyahara, T; Hamanaka, K; Weber, DS; Drake, DA; Anghelescu, M; Parker, JC
      American journal of physiology. Lung cellular and molecular physiology  293  L11-21  2007

      Show Abstract
      17322282 17322282
      In vivo insulin signaling through PI3-kinase is impaired in skeletal muscle of adult rat offspring exposed to ethanol in utero.
      Chen, L; Yao, XH; Nyomba, BL
      Journal of applied physiology (Bethesda, Md. : 1985)  99  528-34  2005

      Show Abstract
      15790685 15790685

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