05-1050X | Anti-Phosphotyrosine Antibody, 4G10® Platinum

05-1050X
30 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AWB, IF, FC, IHC, IP, ICC, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1050X
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphotyrosine Antibody, 4G10® Platinum
      Background InformationThe development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of.
      References
      Product Information
      FormatPurified
      Control
      • Suggested Positive Antigen Control: Cat.# 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at –20ºC. The lysate is stable for 6 months at –20ºC.
      PresentationA proprietary mixture of protein G purified mouse monoclonals 4G10® (IgG2bκ) and PY20 (IgG2b) in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide.
      Applications
      ApplicationAnti-Phosphotyrosine Antibody, 4G10 Platinum is an antibody against Phosphotyrosine for use in WB, IF, FC, IH, IP, IC, ELISA.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Flow Cytometry
      • Immunohistochemistry
      • Immunoprecipitation
      • Immunocytochemistry
      • ELISA
      Biological Information
      Immunogen4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein.
      Clone4G10
      HostMouse
      SpecificityTyrosine-phosphorylated proteins from all species
      IsotypeIgG2b
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodProtein G-Sepharose™ Chromatography
      Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at 2-8°C from date of shipment.
      NOTE: DO NOT FREEZE.
      For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application.
      Packaging Information
      Material Size30 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2003344 2003344
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2243502 2243502
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1601872 DAM1601872
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1821235 DAM1821235
      4G10#174; Platinum, Anti-Phosphotyrosine - 2075953 2075953
      4G10#174; Platinum, Anti-Phosphotyrosine - DAM1462606 DAM1462606
      4G10#174; Platinum, Anti-Phosphotyrosine - JBC1862630 JBC1862630
      4G10#174; Platinum, Anti-Phosphotyrosine - R0709B0208 R0709B0208
      4G10#174; Platinum, Anti-Phosphotyrosine - R0712B0018 R0712B0018
      4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2491203 2491203

      References

      Reference overviewApplicationPub Med ID
      Merlin Isoforms 1 and 2 Both Act as Tumour Suppressors and Are Required for Optimal Sperm Maturation.
      Zoch, A; Mayerl, S; Schulz, A; Greither, T; Frappart, L; Rübsam, J; Heuer, H; Giovannini, M; Morrison, H
      PloS one  10  e0129151  2015

      Show Abstract
      Western Blotting26258444 26258444
      Differential requirement for Src family tyrosine kinases in the initiation, progression, and metastasis of prostate cancer.
      Gelman, IH; Peresie, J; Eng, KH; Foster, BA
      Molecular cancer research : MCR  12  1470-9  2014

      Show Abstract
      25053806 25053806
      Recruitment of Grb2 and SHIP1 by the ITT-like motif of TIGIT suppresses granule polarization and cytotoxicity of NK cells.
      Liu, S; Zhang, H; Li, M; Hu, D; Li, C; Ge, B; Jin, B; Fan, Z
      Cell death and differentiation  20  456-64  2013

      Show Abstract
      23154388 23154388
      Evidence that TMEM67 causes polycystic kidney disease through activation of JNK/ERK-dependent pathways.
      Du, E; Li, H; Jin, S; Hu, X; Qiu, M; Han, R
      Cell biology international  37  694-702  2013

      Show Abstract
      23456819 23456819
      The HPV16 E6 oncoprotein causes prolonged receptor protein tyrosine kinase signaling and enhances internalization of phosphorylated receptor species.
      Spangle, JM; Munger, K
      PLoS pathogens  9  e1003237  2013

      Show Abstract
      Western Blotting23516367 23516367
      Distinct roles of muscle and motoneuron LRP4 in neuromuscular junction formation.
      Wu, H; Lu, Y; Shen, C; Patel, N; Gan, L; Xiong, WC; Mei, L
      Neuron  75  94-107  2012

      Show Abstract
      Western Blotting22794264 22794264
      Protein expression signatures for inhibition of epidermal growth factor receptor-mediated signaling.
      Myers, MV; Manning, HC; Coffey, RJ; Liebler, DC
      Molecular & cellular proteomics : MCP  11  M111.015222  2012

      Show Abstract
      22147731 22147731
      The Kaposi's sarcoma-associated herpesvirus G protein-coupled receptor contains an immunoreceptor tyrosine-based inhibitory motif that activates Shp2.
      Philpott, N; Bakken, T; Pennell, C; Chen, L; Wu, J; Cannon, M
      Journal of virology  85  1140-4  2011

      Show Abstract
      Western Blotting21047965 21047965
      Nifuroxazide inhibits survival of multiple myeloma cells by directly inhibiting STAT3.
      Nelson, EA; Walker, SR; Kepich, A; Gashin, LB; Hideshima, T; Ikeda, H; Chauhan, D; Anderson, KC; Frank, DA
      Blood  112  5095-102  2008

      Show Abstract Full Text Article
      18824601 18824601

      Data Sheet

      Title
      4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b)

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      Catalogue Number Description  
      05-1050 4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b) Show Pricing & Availability

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      Life Science Research > Antibodies and Assays > Primary Antibodies