05-321MG | Anti-Phosphotyrosine Antibody, clone 4G10®

1 mg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AIP, WBMPurifiedMonoclonal Antibody
      Catalogue Number05-321MG
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphotyrosine Antibody, clone 4G10®
      Background InformationSome of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity.
      The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
      Product Information
      • Untreated A-431 (negative control) and EGF treated A-431 (positive control) whole cell lysates.
        Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing PBS, pH 7.4. Liquid at 2-8ºC.
      IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10® (FOX-NY [NS-1 derivative] myeloma x spleen cells).
      ApplicationAnti-Phosphotyrosine Antibody, clone 4G10 detects level of Phosphotyrosine & has been published & validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      2-4 μg of a previous lot immunoprecipitated quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10 cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add: 0.2 mM sodium orthovanadate to the lysis buffer.
      Biological Information
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes tyrosine-phosphorylated proteins from all species.
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      • Phosphorylation
      Purification MethodProtein G-Sepharose™ Chromatography
      Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated on EGF-treated human A431 carcinoma cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; Druker, B. J., 1989; Kanakura, Y., 1991).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.

      For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8ºC. Save the supernatant for application.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Phosphotyrosine, clone 4G10 (mouse monoclonal IgG2bk) - DAM1400107 DAM1400107
      Anti-Phosphotyrosine, clone 4G10#174; - 2040085 2040085
      Anti-Phosphotyrosine, clone 4G10#174; - 704057853 704057853
      Anti-Phosphotyrosine, clone 4G10#174; - DAM1400107 DAM1400107
      Anti-Phosphotyrosine, clone 4G10#174; - DAM1707888 DAM1707888
      Anti-Phosphotyrosine, clone 4G10#174; - DAM1724014 DAM1724014
      Anti-Phosphotyrosine, clone 4G10#174; - DAM1747347 DAM1747347
      Anti-Phosphotyrosine, clone 4G10#174; - DAM1780352 DAM1780352
      Anti-Phosphotyrosine, clone 4G10#174; - JBC1878055 JBC1878055
      Anti-Phosphotyrosine, clone 4G10<sup>reg;</sup> - 29616 29616

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      05-321 Anti-Phosphotyrosine Antibody, clone 4G10® Show Pricing & Availability
      05-321X Anti-Phosphotyrosine Antibody, clone 4G10® Show Pricing & Availability

      Alternative Format

      Catalogue Number Description  
      05-777 Anti-Phosphotyrosine Antibody, recombinant clone 4G10® Show Pricing & Availability
      16-101 Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate Show Pricing & Availability
      16-103 Anti-Phosphotyrosine Antibody, clone 4G10®, Biotin Conjugate Show Pricing & Availability
      16-104 Anti-Phosphotyrosine Antibody, clone 4G10®, FITC conjugate Show Pricing & Availability
      16-105 Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate Show Pricing & Availability
      16-199 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate Show Pricing & Availability
      16-204 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate Show Pricing & Availability
      16-205 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, FITC conjugate Show Pricing & Availability

      Included Positive Control

      Catalogue Number Description  
      12-302 EGF-Stimulated A431 Cell Lysate Show Pricing & Availability

      Product Families


      Life Science Research > Antibodies and Assays > Primary Antibodies